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http://dx.doi.org/10.5713/ajas.2006.297

Effect of IGF-I Rich Fraction from Bovine Colostral Whey on Murine Immunity  

Hwang, Kyung-A (Faculty of Life Science and Technology, Sungkyunkwan University)
Ha, Woel-Kyu (Food Research & Development Lab., Pasteur Milk Co., Ltd.)
Yang, Hee-Jin (Faculty of Life Science and Technology, Sungkyunkwan University)
Lee, Soo-Won (Faculty of Life Science and Technology, Sungkyunkwan University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.2, 2006 , pp. 297-304 More about this Journal
Abstract
Insulin-like growth factor-I (IGF-I) rich fraction, collected components between 1 kDa and 30 kDa, was fractionated from bovine colostral whey using an ultrafiltration membrane. IGF-I was confirmed in the collected IGF-I rich fraction by both SDS-PAGE and Western blotting. The concentration of IGF-I in the IGF-I rich fraction was 10 ng/mg protein. One hundred microliters of the reconstituted IGF-I rich fraction was intraperitoneally injected into ICR male mice for 2 weeks at 24 h intervals. The functions of peritoneal macrophages, including phagocytosis, interleukin (IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ production, and nitric oxide and hydrogen peroxide production, were enhanced significantly by the administration of the IGF-I rich fraction in a dose-dependent manner (p<0.01). The proliferation of Concanavalin (Con) A-stimulated and Lipopolysaccharide (LPS)-stimulated splenocytes was also determined to have been enhanced significantly by the administration of the IGF-I rich fraction in a dose-dependent manner (p<0.01). Our results indicate that the administration of IGF-I rich fraction obtained from bovine colostral whey enhances both innate and acquired immunity for ICR male mice.
Keywords
IGF-I; Colostrum; Macrophage; Splenocyte; Immunity;
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1 Battelli, M. G., A. Abbondanza, S. Musiani, L. Buonamici, P. Strocchi, P. L. Tazzari, L. Gramantieri and F. Stirpe. 1999. Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA). Clin. Chim. Acta. 281:147-158   DOI   ScienceOn
2 Hagiwara, K., S. Kataoka, H. Yamanaka, R. Kirisawa and H. Iwai. 2000. Detection of cytokines in bovine colostrum. Vet. Immunol. Immunopathol. 76:183-190   DOI   ScienceOn
3 Heemskerk, V. H., M. A. R. C. Daemen and W. A. Buurman. 1999. Insulin-like growth factor-1 (IGF-1) and growth hormone (GH) in immunity and inflammation. 10(1):5-14   DOI   ScienceOn
4 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage 4. Nature. 227 (259):680-685   DOI   ScienceOn
5 Nathan, C. F. and R. K. Root. 1977. Hydrogen peroxide release from mouse peritoneal macrophages:dependence on sequential activation and triggering. J. Exp. Med. 146(6):1648-1662   DOI   ScienceOn
6 Stuart, C. A., R. T. Meechan, L. S. Neale, N. M. Cintron and R. W Furlanetto. 1991. Insulin-like growth factor-1 binds selectively to human peripheral blood moncytes and B lymphocytes. J. Clin. Endocrinol. Metab. 72:1117-1122   DOI
7 Warwick-Davies, J., D. B. Lowrie and P. L. Cole. 1995. Growth hormone is a human macrophage activating factor. Priming of human monocytes for enhanced release of $H_2O_2$. J. Immunol. 154:1909-1918
8 Stossel, T. P. 1973. Evaluation of opsonic and leukocyte function with a spectrophotomeric test in patients with infection and with phagocytic disorders. Blood. 42(1):121-130
9 Tapson, V. F., M. Boni-Schnetzler, P. F. Pilch, D. M. Center and J. S. Berman. 1988. Structural and functional characterization of the human T lymphocyte receptor for insulin-like growth factor-1 in vitro. J. Clin. Invest. 82:950-957   DOI
10 Landreth, K. S., R. Narayanan and K. Dorshkind. 1992. Insulinlike growth factor-I regulates pro-B cell differentiation. Blood. 80(5):1207-1212
11 Pakkanen, R. and J. Aalto. 1997. Growth factors and antimicrobial factors of bovine colostrum. Int. Dairy Journal. 7:285-297   DOI   ScienceOn
12 Ha, W. K., D. H. Won, H. J. Yang, K. A. Hwang and S. W. Lee. 2005. Effect of bovine colostrums fractions on the proliferation of mouse splenocytes. Kor. J. Food. Sci. Ani. Resour. 25(2):250-256   과학기술학회마을
13 Holsapple, M. P., P. J. McNerney, A. N. Tucker and T. K. L. White. 1984. Effects of N-nitroso-dimetylamine on humoral immunity. J. Pharmacol. Exp. Ther. 229(2):493-500
14 Klimetzek, V. and H. G. Remold. 1980. The murine bone marrow macrophage, a sensitive indicator cell for murine migration inhibitory factor and a new method for their harvest. Cell Immunol. 53(2):257-266   DOI   ScienceOn
15 Renier, G., I. Clement, A. C. Desfaits and A. Lambert. 1996. Direct stimulatory effects of insulin-like growth factor-1 on monocyte and macrophage tumor necrosis factor-alpha production. Endocrinol. 137:4611-4618   DOI   ScienceOn
16 Hossner, K. L. and R. S. Yemm. 2000. Improved recovery of insulin-like growth factors (IGFs) from bovine colostrum using alkaline diafiltration. Biotechnol. Appl. Biochem. 32(3):161-166   DOI   ScienceOn
17 Ding, A. H., C. F. Nathan and D. J. Stuher. 1988. Release of reactive oxygen intermediates from mouse peritoneal macrophage. Composition of activating cytokines and evidences for independent production. J. Immunol. 141(7):2407-2412
18 Wang, Y. S. and A. Koyama. 2004. Stimulatory effect of IGF-1 and VEGF on eNOS message, protein expression, eNOS phosphorylation and nitric oxide production in rat glomeruli, and involvement of P13-K signaling pathway. Nitric Oxide, 10(1):25-35   DOI   ScienceOn
19 Fu, Y. K., S. Arkins, B. S. Wang and K. W. Kelley. 1991. A novel role of growth hormone and insulin-like growth factor-1. Priming neutrophils for superoxide anion secretion. J. Immunol. 146:1602-1608
20 Green, L. C., D. A. Wagner, J. Glogowski, P. L. Skipper and J. S. Tannenbaum. 1982. Analysis of nitrate, nitrite and [15N] nitrate. Anal. Biochem. 126(1):131-138   DOI   ScienceOn
21 Ortaldo, J. R. and R. B. Hernerman. 1984. Heterogeneity of natural killer cells. Annu. Rev. Immunol. 2:359-394   DOI   ScienceOn
22 Playford, R. J., C. E. Macdonald and W. S. Johnson. 2000. Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders. Am J. Clin Nutr. 72:5-14   DOI
23 Clemmons, D. R. 1998. Role of insulin-like growth factor binding proteins in controlling IGF actions. Molecular and Cellular Endocrinol. 140:19-24   DOI   ScienceOn
24 Frankie, B. S. and A. E. Kitabchi. 2004. De novo emergence of growth factor receptors in activated human $CD4^+$ and $CD8^+$ T lymphocytes. Metabolism. 53:117-122   DOI   ScienceOn
25 Uruakpa, F. O., M. A. H. Ismond and E. A. T. Akobundu. 2002. Colostrum and benefits. Nutrition Research. 22:755-767   DOI   ScienceOn
26 Wenjun, W., O. Kehui, O. Jianhua, L. Haihua, L. Shumao and S. Han. 2004. Polymorphism of Insulin-like Growth Factor I gene in six chicken breeds and its relationship with growth traits. Asian-Aust. J. Anim. Sci. 17(3):301-304   DOI
27 Hossenlopp, P., D. Seurin, B. Segovia-Quinson, S. Hardouin and M. Binoux. 1986. Analysis of serum insulin-like growth factor binding protein using western blotting: use of the method for titration of the binding proteins and competitive binding site. Anal. Biochem. 154(1):138-143   DOI   ScienceOn
28 Flick, D. A. and G. E. Grifford. 1984. Comparison of in vitro cell cytotoxic assay for tumor necrosis factor. J. Immunol. 68:167-175   DOI   ScienceOn
29 Kooijman, R., M. Willems, C. J. De Haas, G. T. Rijkers, A. L. Schuurmans, S. C. Van Buul-Offers, C. J. Heijnen and B. J. Zegers. 1992. Expression of type 1 insulin-like growth factors on human peripheral blood mononuclear cells. Endocrinol. 131:2244-2250   DOI   ScienceOn
30 Tu, W., P. T. Cheung and Y. L. Lau. 1999. IGF-1 increases interferon-gamma and IL-6 mRNA expression and protein production in neonatal mononuclear cells. Pediatr. Res. 46:748-754   DOI   ScienceOn
31 Simmen, F. A., R. C. M. Simmen and G. Reihnart. 1988. Maternal and neonatal somatomedin-C/insulin-like growth factor-I (IGF-I) and IGF binding proteins during early lactation in the pig. Dev. Biol. 130(1):16-27   DOI   ScienceOn
32 Okimura, T., M. Ogawa and T. Yamauchi. 1986. Stress and immune responses III. Effect of restraintstress on delayed type hypersensitivity (DTH) response, natural killer (NK) activity and phagocytosis in mice. Jpn. J. Phamacol. 41(2):229-235