Browse > Article
http://dx.doi.org/10.5713/ajas.2006.1464

Effect of the Mixed Oil and Monensin Supplementation, and Feeding Duration of Supplements on c9,t11-CLA Contents in Plasma and Fat Tissues of Korean Native (Hanwoo) Steers  

Wang, J.H. (College of Bio and Food Technology, Dalian Institute of Light Industry)
Choi, S.H. (Department of Animal Science, Chungbuk National University)
Lim, K.W. (Department of Animal Science, Chungbuk National University)
Kim, K.H. (National Livestock Research Institute, Suwon, RDA)
Song, Man K. (Department of Animal Science, Chungbuk National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.10, 2006 , pp. 1464-1469 More about this Journal
Abstract
The present study was conducted with twenty-four Korean native (Hanwoo) steers to observe the effect of mixed oil and monensin supplementation and duration of feeding on c9,t11-CLA content in plasma and fat tissues. The steers were randomly assigned to three groups of eight animals each according to body weight. Hanwoo steers in the control group were fed the commercial concentrate for the late fattening stage. The other groups of steers were fed the same diet as control steers, but the concentrate was supplemented with high-$C_{18:2}$ oil mixture (soybean oil, sunflower oil, safflower oil) and fish oil at 6% level of concentrate (DM basis), and monensin (20 ppm). The second and third group of steers was fed the oil mixture supplemented diet with monensin for the last 10 weeks and 20 weeks, respectively, prior to being slaughtered. The oil mixture consisted of 45% soybean oil, 20% sunflower oil, 20% safflower oil and 15% fish oil. Average daily gain (p<0.049) and feed efficiency (p<0.018) of the steers fed the diet supplemented with oil mixture and monensin (OM-M) for 20 weeks were higher than those of the other groups of steers. Dressing percent, fat thickness and longissimus muscle area were not affected by the OM-M supplementation and duration of its feeding. The OM-M supplementation increased the content of total-cholesterol (p<0.0001-0.0007) and HDL-cholesterol (p<0.0001) in the plasma of steers compared to the control diet. The steers fed the OM-M diet had a higher proportion of c9,t11-CLA in plasma (p<0.048-0.044) than the control steers. Feeding the OM-M diet for 20 weeks increased the proportion of CLA in intramuscular (p<0.015), intermuscular (p<0.039) and subcutaneous (p<0.001) fat tissues compared with both steers fed the control diet and the OM-M diet for 10 weeks. Increased (p<0.007) proportion of total unsaturated fatty acids in steers fed the OM-M diet for 20 weeks compared to those in control steers was related to the increased (p<0.001) $C_{18:2}$ and decreased (p<0.001) $C_{18:0}$ proportions in subcutaneous tissue.
Keywords
$C_{18:2}$-rich Oils; Monensin; Fish Oil; c9,t11-CLA; Fat Tissue; Hanwoo Steer;
Citations & Related Records

Times Cited By Web Of Science : 11  (Related Records In Web of Science)
Times Cited By SCOPUS : 14
연도 인용수 순위
1 Beaulieu, A. D., J. K. Drackey and N. R. Merchen. 2002. Concentrations of conjugated linoleic acid (cis-9, trans-11- octadecadienoic acid) are not increased in tissue lipids of cattle fed a high-concentrate diet supplemented with soybean oil. J. Anim. Sci. 80:847-861
2 Dhiman, T. R., G. R. Anand, L. D. Satter and M. W. Pariza. 1999. Conjugated Linoleic Acid Content of Milk from Cows Fed Different Diets. J. Dairy Sci. 82:2146-2156   DOI   ScienceOn
3 Goering, K. K. and P. J. Van Soest. 1970. Forage fiber analysis (apparatus, reagents, procedures, and some application). Agric. Handbook 379, ARS, USDA, Washington, DC
4 Lawless, F., J. J. Murphy, D. Harrington, R. Devery and C. Stanton. 1998. Elevation of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk because of dietary supplementation. J. Dairy Sci. 81:3259-3267   DOI   ScienceOn
5 Russel, J. B. 1987. Aproposed model of monnensin action in inhibiting rumen bacteria growth: Effects on ion flux and protonmotive force. J. Anim. Sci. 67:1519
6 Spears, J. W. and R. W. Harvey. 1984. Performance, ruminal and serum characteristics of steers fed lasalocid on pasture. J. Anim. Sci. 58:460-464   DOI
7 Tesfa, A. T., M. Tuori and L. Syrjala-Qvist. 1991. High rapeseed oil feeding to lactating dairy cows and its effect on milk yield and composition in ruminants. Finn. J. Dairy Sci. 49:65-81
8 Wang, J. H., S. H. Choi and M. K. Song. 2003. pH Affects the In Vitro Formation of cis-9,trans-11 CLA and trans-11 Octadecenoic acid by Ruminal Bacteria When Incubated with Oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748   DOI
9 Jiang, J., L. Bjoerk, R. Fonden and M. Emanuelson. 1996. Occurrence of conjugated cis-9,trans-11-octadecadienoic acid in bovine milk: effects of feed and dietary regime. J. Dairy Sci. 79:438-445   DOI   ScienceOn
10 Ivan, M., P. S. Mir, K. M. Koenig, L. M. Rode, L. Neill, T. Entz and Z. Mir. 2001. Effects of dietary sunflower seed oil on rumen protozoa population and tissue concentration of conjugated linoleic acid in sheep. Small Rumin. Res. 41:215-227   DOI   ScienceOn
11 Kelly, M. L., J. R. Berry, D. A. Dwyer, J. M. Griinari, P. Y. Chouinard, M. E. V. Amburgh and D. E. Bauman. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J. Nutr. 128:881-885
12 SAS. 1985. SAS User′s Guide: Statistical Analysis Systems Institute, Inc., Cary, NC
13 Wang, J. H, M. K. Song, Y. S. Son and M. B. Chang. 2002a. Effect of concentrate level on the formation of conjugated linoleic acid and trans-octadecenoic acid by ruminal bacteria when incubated with oilseeds in vitro. Asian-Aust. J. Anim. Sci. 15:687-694   DOI
14 Lepage, G. and C. C. Roy. 1986. Direct transesterification of all classes of lipid in a one-step reaction. J. Lipid Res. 27:114-121
15 Madron, M. S., D. G. Peterson, D. A. Dwyer, B. A. Corl, L. H. Baumgard, D. E. Beermann and D. E. Bauman. 2002. Effect of extruded full-fat soybeans on conjugated linoleic acid content of intramuscular, intermuscular, and subcutaneous fat in beef steers. J. Anim. Sci. 80:1135-1143
16 Zegarska, Z., B. Paszczyk and Z. Borejszo. 1996. Trans fatty acids in milk fat. Pol. J. Food Nutr. Sci. 5:89-96
17 Bauman, D. E., J. W. Perfield II, M. J. de Veth and AlL. Lock. 2003. New perspectives on lipid digestion and metabolism in ruminants. Proc. Cornell Nutr. Conf. pp. 175-189
18 Kott, R. W., P. G. Hatfield, J. W. Bergman, C. R. Flynn, H. Van Wagoner and J. A. Boles. 2003. Feedlot performance, carcass composition, and muscle and fat CLA concentrations of lambs fed diets supplemented with safflower seeds. Small Rumin. Res. 2328:1-7
19 Borsting, C. F., T. Hvelplund and M. R. Weisbjerg. 1992. Fatty acid digestibility in lactating cows fed increasing amounts of protected vegetable oil, fish oil or saturated fat. Acta Agric. Scand., Sect. A, Anim. Sci. 42:148-156
20 Rudel, L. L., J. S. Parks, C. C. Hedrick, M. Thomas and K. Williford. 1998. Lipoprotein and cholesterol metabolism in diet-induced coronary artery atherosclerosis in primates. Role of cholesterol and fatty acids. Prog. Lipid Res. 37(6):353-370   DOI   ScienceOn
21 Lee, K. N., D. Kritchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and artherosclerosis in rabbits. Atherosclerosis. 108:19-25   DOI
22 Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics. Mcgraw Hill Book Co., NY
23 Vonghia, G., A. Viceni, F. Pinto, A. Mastrosimone, A. D. Decandia and G. V. Gnoni. 1997. The utilization of safflower cake, olive residues meal and whey meal in lamb feeding. In: Proceedings of the Fourth International Safflower Conference, Bari, Italy
24 Bauman, D. E., L. H. Baumgard, B. A. Corl and J. M. Griinari. 2000. Biosynthesis of conjugated linoleic acid in ruminants. Proceed. Am. Soc. Anim. Sci. pp. 1-15
25 AOAC. 1995. Official Methods of Analysis (15th ed.). Association of Official Analytical Chemists, Washington, DC
26 Beaulieu, A. D., J. K. Drackey, N. R. Merchen and E. L. Falkenstein. 2000. Concentrations of conjugated linoleic acid in beef carcasses are not increased by supplementing a highcorn diet with 5% soybean oil. J. Anim. Sci. 78 (suppl. 1):285 (Abs.)
27 Scollan, N. D., N. J. Choi, M. Enser and J. D. Wood. 1997. Digestion of linseed and fish oil fatty acids by steers. 48th Annual Meeting of EAAP, Vienna, 24-28th August
28 Timmen, H. and S. Patton. 1988. Milk fat globules: fatty acid composition, size and in vivo regulation of fat liquidity. Lipids. 23:685-689   DOI
29 Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221- 225   DOI
30 Fellner, V., F. D. Sauer and J. K. G. Kramer. 1997. Effect of nigericin, monensin, and tetronasin on biohydrogenation in continous flow-through ruminal fermenters. J. Dairy Sci. 80:921-928   DOI   ScienceOn
31 Stanton, C., F. Lawless, G. Kjellmer, D. Harrington, R. Devery, J. F. Connolly and J. Murphy. 1997. Dietary influences on bovine milk cis-9,trans-11-conjugated linoleic acid content. J. Food Sci. 62:1083-1086   DOI   ScienceOn
32 Ha, Y. L., N. K. Grimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 8:1881-1887   DOI   ScienceOn
33 Wang, J. H. and M. K. Song. 2002b. Effect of concentrate to roughage ratio and oil source on the formation of t-11C18:1 and c-9, t-11C18:2 in rumen fluid and plasma of sheep. The 4th Korea-Japan Joint Symposium on Rumen Metabolism and Physiology in Jeju, Korea. p. 113 (Abs.)
34 Folch, J., M. Lee. and G. H. Sloan-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:497-509
35 Michal, J. J., B. P. Chew, T. D. Schultz and T. S. Wong. 1992. Interaction of conjugated dienoic derivatives of linoleic acid with carotene on cellular host defence. FASEB J. 6, A1102
36 Krehbiel, C. R., R. A. McCoy, R. A. Stock, T. J. Klopfenstein, D. H. Shain and R. P. Huffman. 1995. Influence of grain type, tallow level, and tallow feeding system on feedlot cattle performance. J. Anim. Sci. 73:2916-2921
37 Mir, Z., M. L. Rushfeldt, P. S. Mir, L. J. Paterson and R. J. Weselake. 2000. Effect of dietary supplementation with either conjugated linoleic acid (CLA) or linoleic acid rich oil on the CLA content of lamb tissues. Small Rumin. Res. 36:25-31   DOI   ScienceOn