Browse > Article
http://dx.doi.org/10.5713/ajas.2005.1794

Cholesterol Removal from Homogenized Milk with Crosslinked β-cyclodextrin by Adipic Acid  

Han, Eun-Mi (Department of Food Science and Technology, Sejong University)
Kim, Song-Hee (Department of Food Science and Technology, Sejong University)
Ahn, Joungjwa (Department of Food Science and Technology, Sejong University)
Kwak, Hae-Soo (Department of Food Science and Technology, Sejong University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.18, no.12, 2005 , pp. 1794-1799 More about this Journal
Abstract
The present study was carried out to develop crosslinking of $\beta$-cyclodextrin ($\beta$-CD) using adipic acid, and to determine the optimum conditions of different factors ($\beta$-CD concentration, mixing temperature, mixing time and mixing speed) on cholesterol reduction from homogenized milk. Crosslinked $\beta$-CD was prepared with adipic acid. When the milk was treated with different conditions, the cholesterol removal rate was in the range of 92.1 to 93.1% with 1% $\beta$-CD addition, which were not significantly different among treatments. After cholesterol removal from milk, the used crosslinked $\beta$-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal rate in the first trial was 92.5%, which was mostly same as that using new crosslinked $\beta$-CD. With repeated ten time trials using same sample, 81.4% of cholesterol was removed from milk. Therefore, the present study indicated that the optimum conditions for cholesterol removal using crosslinked $\beta$-CD were 1% $\beta$-CD addition and 10 min mixing with 400 rpm speed at 5$^{\circ}C$ with over 90% cholesterol removal. In addition, crosslinked $\beta$-CD made by adipic acid resulted in the effective recycling efficiency.
Keywords
Crosslinked $\beta$-CD; Cholesterol Removal; Homogenized Milk; Recycling;
Citations & Related Records

Times Cited By Web Of Science : 16  (Related Records In Web of Science)
Times Cited By SCOPUS : 12
연도 인용수 순위
1 Hosney, C. 1986. Principle of cereal science and technology, AACC. St. Paul, MN, USA.
2 Kim, S. H., J. Ahn and H. S. Kwak. 2004. Crosslinking of $\beta$-cyclodexrin on cholesterol removal from milk. Arch. Pharm. Res. 27:1183-1187.
3 Kwak, H. S., S. H. Kim, J. H. Kim, H. S. Choi and J. M. Kang. 2004. Immobilized $\beta$-cyclodextrin as a simple and recyclable method for cholesterol removal in milk. Arch. Pharm. Res. 27:873-877.
4 Kwak, H. S., H. M. Suh, J. Ahn and H. J. Kwon. 2001. Optimization of $\beta$-cyclodextrin recycling process for cholesterol removal in cream. Asian-Aust. J. Anim. Sci. 14:548-552.
5 Nagamoto, S. 1985. Cyclodextrin-expanding the development of their functions and applications. Chem. Econ. Eng. Rev. 17:28-34.
6 Shim, S. Y., J. Ahn and H. S. Kwak. 2003. Functional properties of cholesterol-removed whipping cream treated by $\beta$-cyclodextrin. J. Dairy Sci. 86:2767-2772.
7 Yen, G. C. and L. T. Tsui. 1995. Cholesterol removal from lardmixture with $\beta$-cyclodextrin. J. Food Sci. 60:561-564.
8 Makoto, K., O. Akio and S. Reijiro. 1992. Cholesterol removal from animal fat with cyclodextrin in inclusion. Honnen LTD., assignee, Japan Pat. No. 4:168,198.
9 Adams, M. L., D. M. Sullivan, R. L. Smith and E. F. Richer. 1986. Evaluation of direct saponification method in determination of cholesterol in meats. J. Assoc. Anal. Chem. 69:844-846.
10 Carleton, R. A., L. Finberg, J. Flora, D. S. Goodman, S. M. Grundy, S. Haven, G. T. Hunter, D. Kritchevsky, R. M. Lauer, R. V. Luepker, A. G. Ramirez, L. Van Horn, W. B. Stason and J. Stokes. 1991. Report of the expert panel on population strategies for blood cholesterol reduction. Circulation 83:2154-2232.
11 Lee, J. S., Z. Ustunol and D. M. Smith. 1993. Cholesteorl removal from cream using $\beta$-cyclodextrin and derivatives. J. Dairy Sci. 76(Suppl.1):143 (Abstr.).
12 Oh, H. I., E. J. Jang and H. S. Kwak. 1998. Conditions of the removal of cholesterol from milk by treatment with saponin. Korean J. Dairy Sci. 20:253-260.
13 Sieber, R. 1993. Cholesterol removal from animal food - Can it be justified'? CH-3097 Fed. Dairy Res. Inst. Liebefeld, Switzerland. pp. 375-387.
14 Lee, S. J., J. S. Hwang, S. Lee, J. Ahn and H. S. Kwak. 2005. Property changes and cholesterol lowering effect in evening primrose oil-added and cholesterol-reduced yogurt. J. Dairy Sci. (in press).
15 Hwang, J. H., S. J. Lee and H. S. Kwak. 2005. Change of properties and cholesterol lowering effect in evening primrose oil-added and cholesterol-reduced milk. Asian-Aust. J. Anim. Sci. 18:1041-1047.
16 Micich, T. J. 1990. Behaviors of polymer supported digitonin with cholesterol in the absence and presence of butter oil. J. Agric. Food Chem. 38:1839-1843.   DOI
17 Fleche, G. 1985. Chemical modification and degradation of starch. In: (Ed. G. M. Van Beynum and J. A. Roel) Starch conversion technology. Dekker, New York, NY, USA, pp. 73-99.
18 Schroder, B. G. and R. J. Baer. 1990. Consumer evaluation of reduced-cholesterol butter. Food Technol. Oct, 104-107.
19 Grundy, S. M., D. Brheimer, H. Blackburn, W. V. Brown, P. O. Kwiterovich, F. Mattson, G. Schonfeld and W. H. Weidman. 1982. Rational of the diet-heart statement of the American Heart Association Report of the Nutrition Committee. Circulation 65:839A-854A.
20 Pyorala, K. 1987. Dietary cholesterol in relation to plasma cholesterol and coronary heart disease. Am. J. Clin. Nutr. 45:1176-1184.
21 Shim, S. Y., J. Ahn and H. S. Kwak. 2004. Functional properties of cholesterol-removed compound whipping cream by palm oil. Asian-Aust. J. Anim. Sci. 17:857-862.
22 Gurr, M. 1992. Dietary lipids and coronary disease: old evidence, new perspective and progress. Lipid Res. 31:195-243.
23 Kwak, H. S., C. S. Jung, S. Y. Shim and J. Ahn. 2002. Removal of cholesterol from Cheddar cheese by $\beta$-cyclodextrin. Agric. Food Chem. 50:7293-7298.
24 Oakenfull, D. G. and G. S. Sihdu. 1991. Cholesterol reduction. Commonwealth scientific and industrial research organization. Int. Pat. No. 91/11114.
25 Kwak, H. S., H. J. Ahn and J. Ahn. 2005. Development of phytosterol ester-added Cheddar cheese for lowering blood cholesterol. Asian-Aust. J. Anim. Sci. 18:267-276.
26 Luallen, E. T. 1985. Starch as a functional ingredient, Food Technol. 39:59-63.
27 Szejtli, J. 1982. Chemistry and separation of cyclodextrins. In: (Ed. J. Szjetli) Cyclodextrins and their inclusion complexes. Akademiai Kiado, Budapest, Hungry. pp. 17-43.
28 Hamerstrand, G. E., B. T. Hofreiter and C. L. Mehltretter. 1959. Determination of the extent of reaction between epichlorohydrin and starch. 37:519-524
29 Kim, J. J., S. H. Yoo, J. Ahn, W. M. Jeon and H. S. Kwak. 2005. The effect of evening primrose oil on chemical properties and lowering blood cholesterol in nutraceutical Cheddar cheese. Arch. Pharm. Res. (in press).
30 SAS$^{\circledR}$. 1985. Users Guide: Statistics, Version 5 Edition. SAS Inst., Inc., Cary, NC, USA.
31 Whistler, R. and R. Daniel. 1990. Function of polysaccharide. In: Food additives, Dekker, New York, NY, USA. pp. 399-406.