[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5713/ajas.2005.1513

Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species

Chan, S.C. (Department of Biology, Faculty of Science, Universiti Putra Malaysia)
Tan, S.G. (Department of Biology, Faculty of Science, Universiti Putra Malaysia)
Siraj, S.S. (Department of Biology, Faculty of Science, Universiti Putra Malaysia)
Yusoff, K. (Department of Microbiology, Faculty of Biotechnology and Biomolecular)

Publication Information

Asian-Australasian Journal of Animal Sciences / v.18, no.11, 2005 , pp. 1513-1518 More about this Journal

Abstract

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

Keywords

Microsatellites; Cross-species Amplification; Mystus nemurus; Pangasius micronemus; Clarias batrachus;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)

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