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http://dx.doi.org/10.5713/ajas.2004.1088

Effect of α-Tocopherol Supplementation on Plasma Levels of Antioxidant Vitamins in Anestrus Buffalo Heifers (Bubalus bubalis)  

Kahlon, R.S. (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
Singh, Rajvir (Department of Veterinary Physiology, College of Veterinary Science, Punjab Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.17, no.8, 2004 , pp. 1088-1092 More about this Journal
Abstract
The present investigation was undertaken to study the status of plasma antioxidant vitamins in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. The pre-supplementation plasma levels ($\mu$mol/L) of vitamin E and $\beta$-carotene were significantly (p<0.05) lower and of vitamin C was significantly (p<0.05) higher in anestrus heifers (4.06$\pm$0.07; 4.56$\pm$0.17; 21.04$\pm$0.21) when compared to normal cycling ones (4.92$\pm$0.05; 6.76$\pm$0.12; 14.24$\pm$0.16). The oral supplementation of$\alpha$-tocopherol at 3,000 mg per week per animal in anestrus heifers resulted in a significant (p<0.01) increase in vitamin E and $\beta$-carotene levels and a significant (p<0.01) decrease in vitamin C concentration. Results indicated that supplementation of $\alpha$-tocopherol to anestrus buffalo heifers improved the antioxidant status by mitigating the harmful effects of free radical induced oxidative stress.
Keywords
Antioxidant Vitamins; $\alpha$-Tocopherol; Anestrus; Buffalo Heifers;
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1 Burton, G. W. and K. U. Ingold. 1984. $\beta$-Carotene: an unusual type antioxidant. Science 224:569-573.
2 Goto, K., O. Kajisa, K. Ezoe, Y. Nakanishi, A. K. Ogaw, M. Tasaki, H. Ohta, S. Inohae, S. Tateyama and T. Kawabata. 1989. Relationship between plasma $\beta$-carotene concentration and embryo quality in superovulated Japanese Black cattle. Memoirs Fac. Agril Kagoshima Univ.25:113-117.
3 Grandhi, R. P., M. W. Smith, M. Frigg and P. A. Thacker. 1993. Effect of supplemental vitamin E during prepubertal development and early gestation on reproductive performance and nutrient metabolism in gilts. Can. J. Anim. Sci. 73:593-603.
4 Jukola, E., J. Hakkarainen, H. Saloniemi and S. Sankari. 1996. Blood selenium, vitamin E, Vitamin A and $\beta$-carotene concentrations and udder development, fertility treatments and fertility. J. Dairy Sci. 79:838-845.
5 Liebler, D. C. and J. A. Burr. 1995. Antioxidant stoichiometry and the oxidative fate of vitamin E in peroxy radical scavenging reactions. Lipids 30:789-793.
6 Krinsky, N. I. 1993. Actions of carotenoids in biological systems. Ann. Rev. Nutr. 13:561-587.   DOI   PUBMED   ScienceOn
7 Wang, J. Y., C. B. Hafi and L. L. Larson. 1988. Effect of supplemental $\beta$-carotene on luteinizing hormone released in response to gonadotropin-releasing hormone challenge in ovariectomized Holstein cows. J. Dairy Sci. 71:498-504.
8 Machlin, L. J. and A. Bendich. 1987. Free radical tissue damage: protective role of antioxidant nutrients. Fed. Am. Soc. Exp. Biol. J. 1: 441-445.
9 Chan, A. C. 1993. Partners in defense, Vitamin E and Vitamin C. J. Physiol. Pharmacol. 71:725-731.   DOI   ScienceOn
10 Machlin, L. J. 1991. Vitamin E. In: (Ed. L. J. Machlin) Handbook of Vitamins. 2nd edn, pp. 99-144. Marcel Dekker, New York.
11 Kahlon, R. S. 1998. Studies on antioxidant status of normal cycling, delayed pubertal and anoestrus buffaloes (Bubalus bubalis). Ph. D. dissertation, Punjab Agricultural University, Ludhiana, India.
12 Kahlon,R. S., S. P. S. Sodhi, Singh Rajvir and Singh Narinder 2002. Osmotic fragility of erythrocytes in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers. SARAS J. Livestock & Poultry Production 18 (3-4):30-35.
13 Weiss, W. P., J. S. Hogan, K. L. Smith and S. N. Williams. 1994. Effect of dietary fat and vitamin E on $\alpha$-tocopherol and $\beta$-carotene in blood of peripartum cows. J. Dairy Sci. 77:1422-1429.
14 Stahl, W. and H. Sies. 1993. Physical quenching of singlet oxygen and cis – trans isomerization of carotenoids. Ann. New York Acad. Sci. 691: 10-19.   DOI
15 Mert, N., R. Vural, U. Gunsen and M. Akandir. 1992. Relationship of anoestrus with plasma $\beta$-carotene, vitamin A and vitamin E levels in Thoroughbred crosses. Veteriner Fakultesi Dergisi Uludag Universitesi 11:19-23.
16 Larson, R. A. 1997. Carotenoids and related polyenes. In: (Ed. R. A. Larson) Naturally Occurring Antioxidants. pp. 179-189. Lewis Publishers, New York.
17 Papas, A. M. 1996. Determinants of antioxidant status in humans. Lipids 31:S77-S82.
18 Garnsworthy, P. C. 1988. Nutrition and Lactation in the Dairy cow. pp. 140-145. Butterworths, London.
19 Kayden, H. J., C. K. Chow and L. K. Bjornson. 1973. Spectrophotometric method for determination of tocopherol in red blood cells. J. Lipid Res. 14:533-540.
20 Draper, H. H. 1990. Nutritional modulation of oxygen radical pathology. Adv. Nutr. Res. 8:119-145.   PUBMED
21 Kahlon, R. S. and R. Singh. 2003. Status of antioxidant enzymes in normal cycling and $\alpha$-tocopherol supplemented anestrus buffalo heifers (Bubalus bubalis). Asian-Aust. J. Anim. Sci. 16(2):217-221.
22 Lotthammer, K. H. 1975. Eierstocks-und Gebarmuttererkrankungen bei subklinischen stoffwechselstorungen der Milchkuhe. Praktische Tierarzt Sonderheft collegium Veternarium 56:24-29.
23 Lotthammer, K. H., B. C. Cook and H. Friesecke. 1978. Importance of $\beta$-carotene for bovine fertility. In: Roche Symposium. London, Roche, Switzerland.
24 Baker, H. and O. Frank. 1968. Clinical Vitaminology: Methods and Interpretation. pp. 153-168. Interscience Publishers, London.
25 Barnes, M. M. C. and A. J. Smith. 1975. The effect of vitamin E deficiency on some enzymes of steroid hormone biosynthesis. Int. J. Vit. Nutr. Res. 45:396-402.
26 Ezzo, O. H. 1995. The effect of vitamins and selenium supplementation on serum vitamin levels and some reproductive patterns in Egyptian buffaloes during pre and postpartum periods. Buffalo J. 11:103-107.
27 Rock, C. L., R. A. Jacob and P. E. Bowen.. 1996. Update on the biological characteristics of the antioxidant micronutrients: vitamin C, vitamin E and the carotenoids. J. Am. Diet Assoc. 96:693-702.