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http://dx.doi.org/10.5713/ajas.2004.712

Separation of Calcium-binding Protein Derived from Enzymatic Hydrolysates of Cheese Whey Protein  

Kim, S.B. (Dairy Science Major, Division of Animal Science, College of Agriculture and Life Sciences Gyeongsang National University)
Shin, H.S. (Nam Yang Research and Development Center)
Lim, J.W. (Dairy Science Major, Division of Animal Science, College of Agriculture and Life Sciences Gyeongsang National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.17, no.5, 2004 , pp. 712-718 More about this Journal
Abstract
This study was carried out to separate the calcium-binding protein derived from enzymatic hydrolysates of cheese whey protein. CWPs (cheese whey protein) heated for 10 min at $100^{\circ}C$ were hydrolyzed by trypsin, papain W-40, protease S, neutrase 1.5 and pepsin, and then properties of hydrolysates, separation of calcium-binding protein and analysis of calcium-binding ability were investigated. The DH (degree of hydrolysis) and NPN (non protein nitrogen) of heated-CWP hydrolysates by commercial enzymes were higher in trypsin than those of other commercial enzymes. In the result of SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis), $\beta$-LG and $\alpha$-LA in trypsin hydrolysates were almost eliminated and the molecular weight of peptides derived from trypsin hydrolysates were smaller than 7 kDa. In the RP-HPLC (reverse phase HPLC) analysis, $\alpha$-LA was mostly eliminated, but $\beta$-LG was not affected by heat treatment and the RP-HPLC patterns of trypsin hydrolysates were similar to those of SDS-PAGE. In ion exchange chromatography, trypsin hydrolysates were shown to peak from 0.25 M NaCl and 0.5 M NaCl, and calcium-binding ability is associated with the large peak, which was eluted at a 0.25 M NaCl gradient concentration. Based on the results of this experiment, heated-CWP hydrolysates by trypsin were shown to have calcium-binding ability.
Keywords
Cheese Whey Protein; Calcium-binding Protein; Enzymatic Hydrolysates;
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