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http://dx.doi.org/10.5713/ajas.2004.1491

Association of a Single Codon Deletion in Bone Morphogenetic Protein 15 Gene with Prolificacy in Small Tail Han Sheep  

Guo, W. (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Chu, M.X. (Institute of Animal Science, Chinese Academy of Agricultural Sciences)
Deng, X.M. (College of Animal Science and Technology, China Agricultural University)
Feng, J.D. (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Li, Ning (State Key Laboratory for Agrobiotechnology, China Agricultural University)
Wu, Changxin (College of Animal Science and Technology, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.17, no.11, 2004 , pp. 1491-1495 More about this Journal
Abstract
Small Tail Han Sheep has significant characteristics of high prolificacy and non-seasonal ovulatory activity and is an excellent local sheep breed in P. R. China. Recently a novel member of the transforming growth factor $\beta$ (TGF$\beta$) superfamily termed bone morphogenetic protein 15 (BMP15) was shown to be specifically expressed in oocytes and to be essential for female fertility. Therefore, BMP15 is a candidate gene for reproductive performance of Small Tail Han Sheep. The whole genomic nucleotide sequence of BMP15 gene in Small Tail Han Sheep was searched for polymorphisms by PCR-SSCP and direct sequencing, and only one polymorphism was found. The polymorphism was a result of a 3 base pair deletion, which eliminated a single Leu codon (CTT). The allelic frequencies for A (without deletion) and B (with a codon deletion) are 0.73 and 0.27 respectively. The effects of BMP15 genotype on litter size were evaluated using the least squares model. This indicated that there was a significant association between litter size of Small Tail Han Sheep and a deletion in BMP15 gene (p=0.02<0.05). Small Tail Han Sheep ewes with AA and AB genotype produce on average 0.5 and 0.3 more lambs per litter than those ewes with BB genotype.
Keywords
Sheep; Prolificacy; Bone Morphogenetic Protein 15 Gene; PCR-SSCP;
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