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http://dx.doi.org/10.5713/ajas.2003.1087

Detection of Imprinted Quantitative Trait Loci (QTL) for Growth Traits in Pigs  

Lee, H.K. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
Lee, S.S. (Department of Animal Science, University of Sydney)
Kim, T.H. (National Livestock Research Institute, Rural DevelopmentAdministration)
Jeon, G.J. (Department of Genomic Engineering, Genomic Informatics Center, Hankyong National University)
Jung, H.W. (Department of Animal Husbandry, Yonam College ofAgriculture)
Shin, Y.S. (Shinku College)
Han, J.Y. (Department of Animal Science and Technology, Seoul NationalUniversity)
Choi, B.H. (National Livestock Research Institute, Rural Development Administration)
Cheong, I.C. (National Livestock Research Institute, Rural Development Administration)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.16, no.8, 2003 , pp. 1087-1092 More about this Journal
Abstract
As an experimental reference population, crosses between Korean native pig and Landraces were established and information on growth traits was recorded. Animals were genotyped for 24 microsatellite markers covering chromosomes 2, 6, and 7 for partial-genome scan to identify chromosomal regions that have effects on growth traits. quantitative trait loci (QTL) effects were estimated using interval mapping by the regression method under the line cross models with a test for imprinting effects. For test of presence of QTL, chromosome-wide and single position significance thresholds were estimated by permutation test and normal significance threshold for the imprinting test were derived. For tests against the Mendelian model, additive and dominance coefficients were permuted within individuals. Thresholds (5% chromosome-wide) against the no-QTL model for the analyzed traits ranged from 4.57 to 4.99 for the Mendelian model and from 4.14 to 4.67 for the imprinting model, respectively. Partial-genome scan revealed significant evidence for 4 QTL affecting growth traits, and 2 out of the 4 QTLs were imprinted. This study demonstrated that testing for imprinting should become a standard procedure to unravel the genetic control of multi-factorial traits. The models and tests developed in this study allowed the detection and evaluation of imprinted QTL.
Keywords
Pig; QTL; Imprinting; Microstellite Markers;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 9
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1 Knott, S. A., L. Marklund, C. S. Haley, K. Andersson, W. Davies, H. Ellegren, M. Fredholm, I. Hansson, B. Hoyheim, K. Lundstrom, M. Moller and L. Andersson. 1998. Multiple marker mapping of quantitative trait loci in a cross between outbred wild boar and large white pigs. Genetics 149:1069-1080.
2 De Vries, A. G., R. Kerr, B. Tier, and T. Long. 1994. Gametic imprinting effects on rate and composition of pig growth. Theor. Appl. Genet. 88:1037-1042.
3 Wang, L., T. P. Yu, C. K. Tuggle, H. C. Liu, and M. F. Rothschild. 1998. A directed search for quantitative trait loci on chromosomes 4 and 7 in the pig. J. Anim. Sci. 76:2560-2567.
4 Joen, J. T., O. Carlborg, A. Tornsten, E. Giuffra, V. Amarger, P. Chardon, L. Andersson, K. Andersson, I. Hansson, K. Lundstrom, and L. Andersson. 1999. A paternally expressed QTL affecting skeletal and cardiac muscle mass in pig mass to the IGF-2 locus. Nature Genet. 21:157-158.   DOI   ScienceOn
5 Nezer, C., L. Moreau, B. Brouwer, W. Coppieters, J. Detilleux, R. Hanset, L. Karim, P. Kvasz, A. Leroy and M. George. 1999. An imprinted QTL with major effect on muscle mass and fat deposition maps to the IGF2 locus in pig. Nat. Genet. 21:155-156.   DOI   ScienceOn
6 Andersson, L., C. S. Haley, H. Ellegren, S. A. Knott and M. Johansson. 1994. Genetic mapping of quantitative trait loci for growth and fatness in pigs. Science 263:1771-1774.   DOI
7 Haley, C. S., S. A. Knott and J. M. Elson. 1994. Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics 136:1195-1207.
8 Lee, H. K. and G. J. Jeon. Detection of significance threshold for detecting QTL. 2002. J. Anim. Sci. & Technol. (Kor) 44:31-38.   DOI
9 Green, P., I. Fall, and S. Crook. 1990. Documentation for CRIMAP version 2.4. Washington University School of Medicine, St. Louis, MO.
10 Churchill, G. A. and R. W. Doerge. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138:963-931.
11 Rattink, A. P., D. J. de Koning, M. Faivare, B. Harlizius, A. M. Johan, J. A. M. van Arendonk, and M. A. M. Groenen. 2000. Fine mapping and imprinting analysis for fatness trait QTLs in pigs. Mamm. Genome 11:656-661.   DOI   ScienceOn
12 Seaton, G., C. S. Haley, S. A. Knott, M. Kearsey and P. M. Visscher. 2002. QTL Express: mapping quantitative trait loci in simple and complex pedigrees. Bioinformatics Applications Note. 18:339-340.   DOI   ScienceOn
13 De Koning, D. J., A. P. Rattink, B. Harlizius, A. M. Johan, J. A. M. Van Arendonk, E. W. Brascamp, and M. A. M. Groenen. 2000. Genome-wide scan for body composition in pig reveals important role of imprinting. Proc. Natl. Acad. Sci. USA. 97:7947-7950.   DOI   ScienceOn