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http://dx.doi.org/10.5713/ajas.2003.1701

Association between PCR-RFLP of Melatonin Receptor 1a Gene and High Prolificacy in Small Tail Han Sheep  

Chu, M.X. (Institute of Animal Science, Chinese Academy of Agricultural Sciences)
Ji, C.L. (College of Animal Science and Veterinary Medicine, Yangzhou University)
Chen, G.H. (College of Animal Science and Veterinary Medicine, Yangzhou University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.16, no.12, 2003 , pp. 1701-1704 More about this Journal
Abstract
Melatonin regulates circadian rhythms and reproduction changes in seasonally reproductive mammals through binding to high-affinity, G-protein-coupled receptors. Small Tail Han sheep that has significant characteristics of high prolificacy and nonseasonal ovulatory activity is an excellent local sheep breed in P. R. China. The exon 2 of the ovine melatonin receptor 1a (MTNR1A) gene was amplified and a uniform fragment of 824 bp was obtained in 150 ewes of Small Tail Han sheep. The 824 bp PCR product was digested with restriction endonucleases Mnl I and Rsa I, and genetic polymorphism was detected by PCR-RFLP. Polymorphic Mnl I site was detected at base position 605 of the exon 2 of the MTNR1A gene. There were two kinds of genotypes in Small Tail Han sheep, AB (303 bp, 236 bp/67 bp) and BB (236 bp/67 bp, 236 bp/67 bp). The results indicated that genotype AA (303 bp, 303 bp) at Mnl I-RFLP site did not exist in non-seasonal estrous Small Tail Han sheep, which suggested that there was an association between genotype AA (303 bp, 303 bp) and reproductive seasonality in sheep. Polymorphic Rsa I site was detected at base position 604 of the exon 2 of the MTNR1A gene. Three kinds of genotypes were found in Small Tail Han sheep, AA (290 bp, 290 bp), AB (290 bp, 267 bp/23 bp) and BB (267 bp/23 bp, 267 bp/23 bp). Least squares means of litter size in the first parity and the second parity for genotype AA (290 bp, 290 bp) at Rsa I-RFLP site were 0.43 and 1.06 more than those for genotype AB (290 bp, 267 bp/23 bp) in Small Tail Han sheep.
Keywords
Sheep; Prolificacy; Reproductive Seasonality; Melatonin Receptor 1a Gene; PCR-RFLP;
Citations & Related Records

Times Cited By Web Of Science : 13  (Related Records In Web of Science)
Times Cited By SCOPUS : 16
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1 Reppert, S. M., D. R. Weaver, S. A. Rivkees and E. G. Stopa. 1988. Putative melatonin receptors in a human biological clock. Science 242:78-81.
2 Reppert, S. M., D. R. Weaver and T. Ebisawa. 1994. Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron. 13:1177-1185.
3 SAS Institute Inc. 2000. SAS/STAT User’s Guide: Version 8. 1st edn. SAS Institute Inc., Cary, North Carolina, USA.
4 Wang, J. Y., J. X. Li and J. C. Wei. 1990. Selection and improvement on Small Tail Han sheep. China Sheep and Goat Farming (1):1-3.
5 Messer, L. A., L. Wang, C. K. Tuggle, M. Yerle, P. Chardon, D. Pomp, J. E. Womack, W. Barendse, A. M. Crawford, D. R. Notter and M. F. Rothschild. 1997. Mapping of the melatonin receptor 1a (MTNR1A) gene in pigs, sheep, and cattle. Mammalian Genome 8:368-370.
6 Klein, D. C., R. Y. Moore and S. M. Reppert. 1991. ‘Suprachiasmatic Nucleus: The Mind’s Clock’. Oxford Press, New York.
7 Slaugenhaupt, S. A., A. L. Roca, C. B. Liebert, M. R. Altherr, J. F. Gusella and S. M. Reppert. 1995. Mapping of the gene for the $Mel_{1a}$-melatonin receptor to human chromosome 4 (MTNR1A) and mouse chromosome 8 (MTNR1A). Genomics 27:355-357.
8 Barrett, P., S. Conway, R. Jockers, A. D. Strosberg, B. Guardiola-Lemaitre, P. Delagrange and P. J. Morgan. 1997. Cloning and functional analysis of a polymorphic variant of the ovine $Mel_{1a}$ melatonin receptor. Biochem. Biophys. Acta. 1356:299-307.
9 Zheng, P. L. 1989. Breed Records of Chinese Sheep and Goats. Shanghai Science and Technology Press, Shanghai, P. R. China.
10 Kokkola, T., M. A. Watson, J. White, S. Dowell, S. M. Foord and J. T. Laitinen. 1998. Mutagenesis of human $Mel_{1a}$ melatonin receptor expressed in yeast reveals domains important for receptor function. Biochem. Biophys. Res. Commun. 249:531-536.
11 Vanecek, J. 1988. Melatonin binding sites. J. Neurochem. 51:1436-1440.
12 Pelletier, J., L. Bodin, E. Hanocq, B. Malpaux, J. Teyssier, J. Thimonier and P. Chemineau. 2000. Association between expression of reproductive seasonality and alleles of the gene for $Mel_{1a}$ receptor in the ewe. Biol. Reprod. 62:1096-1101.
13 Ebisawa, T., N. Kajimura, M. Uchiyama, M. Katoh, M. Sekimoto, T. Watanabe, Y. Ozeki, M. Ikeda, T. Jodoi, M. Sugishita, T. Iwase, Y. Kamei, K. Kim, K. Shibui, Y. Kudo, N. Yamada, R. Toyoshima, M. Okawa, K. Takahashi and T. Yamauchi. 1999. Allelic variants of human melatonin 1a receptor: function and prevalence in subjects with circadian rhythm sleep disorders. Biochem. Biophys. Res. Commun. 262:832-837.
14 Migaud, M., S. Gavet and J. Pelletier. 2002. Partial cloning and polymorphism of the $melatonin_{1a}$ ($Mel_{1a}$）receptor gene in two breeds of goat with different reproductive seasonality. Reproduction 124:59-64.
15 Weaver, D. R. and S. M. Reppert. 1996. The $Mel_{1a}$ melatonin receptor gene is expressed in human suprachiasmatic nuclei. Neuroreport 8:109-112.
16 Chu, M. X., J. Z. Wang, A. G. Wang, N. Li and j. l. Fu. 2003. Association analysis between five microsatellite loci and litter size in Small Fail Han sheep. Asian-Aust. J. Anim. Sci. 16(11):1555-1559.
17 Dubocovich, M. L. and J. S. Takahashi. 1987. Use of 2-[$^{125}I$] iodomelatonin to characterize melatonin binding sites in chicken retina. Proc. Natl. Acad. Sci. USA 84:3916-3920.
18 Roca, A. L., C. Godson, D. R. Weaver and S. M. Reppert. 1996. Structure, characterization, and expression of the gene encoding the mouse $Mel_{1a}$ melatonin receptor. Endocrinology 137:3469-3477.