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Diesel Exhaust Particles and Airway Inflammation: Effect of Nitric Oxide Synthase Inhibitors  

Lim, Heung-Bin (Department of Tobacco Science Chungbuk National University)
Lee, Dong-Wook (Laboratory of Biochemistry Korea Ginseng and Tobacco Research Institute Sshinsung-Dong)
Publication Information
Journal of Korean Society for Atmospheric Environment / v.18, no.E2, 2002 , pp. 121-128 More about this Journal
Abstract
This study was carried out to investigate if nitric oxide synthase (NOS) inhibitors modulate airway inflammation induced by diesel exhaust particles (DEP). N$\^$G/-nitro-L-arginine methyl ester (L-NAME), a potent constitutive NOS (cNOS) inhibitor, and aminoguanidine (AG), a selective inducible NOS (iNOS) inhibitor, were administered to mice in their drinking water for 7 weeks. Airway inflammation was elicited by the repeated intratracheal administration of DEP. The results showed that macrophages, inflammatory eosinophils and neutrophils in bronchoalveolar lavage (BAL) fluids by intratracheal DEP instillation were significantly suppressed in the mice treated with two NOS inhibitors toghther with DEP. The suppression of these cells was more effective in AG treated groups than in L -NAME treated groups. NOS inhibitor treatment also reduced interleukin -5 (IL-5 in the BAL fluids and lung homogenates. Additionally, it was found that eosinophil peroxidase (EPO) activity in the BAL fluids was also decreased by NOS inhibitor treatment. These results suggest that nitric oxide (NO) is produced in airway inflammation by repeated DEP instillation, and that iNOS inhibition as well as cNOS inhibition can play a modulating role in this airway inflammation by DEP.
Keywords
diesel exhaust particles (DEP); airway inflammation; nitric oxide synthase (NOS) inhibitor;
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1 Connor, J.R., P.T. Manning, S.L. Settle, W.M. Moore, G.M. Jerome, R.K. Webber, F.S. Tjoeng. and M.G. Cuuie(1995) Suppression of adjuvant-induced arthritis by selective inhibition of inducible nitric oxide synthase. Eur. J. Phannacol. 273, 15-24
2 Folkerts, G., J. Kloek, R.B. Muijsers, and F.P. Nijkamp(2001) Reactive nitrogen and oxygen species inairway in airway inflammation. Eur. J. Phannacol. 429, 251-262
3 Joly, G.A., M. Ayres, F. Chelly, and R.G. Kilboum (1994) Effects of N-methyl-L-arginine. N-nitro-L-arginine and aminoguanidine on constitutive and inducible nitric oxide synthase in rat aorta. Biochem. Biophys. Res. Comm. 199, 147-154
4 Khantonov, S.A., D.H. Yates, and P.J. Bames (1996) Inhaled glucocorticoids decrease nitric oxide inhaled air of asthmatic patients. Am. J. Respir. Crit. Care & Med. 153. 454-457
5 Kita, H., S. Sur, L.W. Hunt, E.S. Edell, D.A. Weiler, M.C. Swanson, R.W. Samsel, J.S. Abrams, and G.J. Gleich(1996) Cytokine production at the site of disease in chronic eosinophillic pneumonitis. Am. J. Respir. Crit. Care Med. 153, 1437-1441
6 Kumagai, Y., T. Arimoto, M. Shinyashiki, N. Shimojo, Y. Nakai, T. Yoshikawa. and M. Sagai(1997) Generation of reactive oxygen species during interaction of diesel exhaust particle components with NADPH-cytochrome P450 reductase and involvement of the bioactivation un the DNA damage. Free Rad. Biol. Med. 22(3), 479-487
7 Pederson, B., M. Lversen. L.B. Bundgaard, and R. Dahl(1996) Pig fanner have signs of bronchial inflammation and increased numbers of lymphocytes and neutrophils in BAL fluid. Eur. Respir. J. 9, 524-530
8 Takano, H., T. Ichinose, Y. Miyabara, T. Shibuya, H.B. Lim, T. Yoshikawa, and M. Sagai(1998a) Inhallation of diesel exhaust enhances allergen-related eosino-phil recruitment and airway hyperresponsiveness in mice. Toxicol. Appl. Pharmacol. 150(2), 328-338
9 Van Ooslerhout, A.J.M., I. Van Ark, G. Hofman, H.J. Van Der Linde, D. Fattah, and F.P. Nijkamp (1996) Role of interleukin-5 and substance P in development of airway hyperreactivity to histamine in guinea-pigs. Eur. Respir. J. 9, 493-499
10 Akaike, T., Y. Noguchi, S. Ijiri, K. Setoguchi, M. Suga, Y.M. Zheng, B. Dietzschold, and H. Maeda(1996) Pathogenesis of influenza virus-induced pneumonia: Involvement of both nitric oxide and oxy-gen radicals. Proc. Natl. Acad. Sci. 93, 2448-2453
11 Alving, K., E. Weitzberg, and J.M. Lundberg (1994) Increased amount of nitric oxide in exhaled air of asthma-tics. Eur. Respir. J. 6, 1368-1370
12 Johnston, C.J., J.N. FinkeIstein, R. Baggs, and G. Oberdorster(1996) Characterization of the early pulmonary inflammatory response associated with PTFE fume exposure. Toxicol. Appl. PharmacoI. 109, 235-240
13 Miyabara. Y., H. Takano, T. Ichinose, H.B. Lim, and M. Saeai (1998b) Diesel exhaust enhances allergic airway inflammation and hyperresponsiveness in mice. Am. J. Respir. Crit. Care Med. 157(4 Pt 1), 1138-1144
14 Grisham, M.B., R.D. Specian, and T.E. Zinimemian (1994) Effecs of nitric oxide synthase inhibition on the pathophysiology observed in a model of chronicgranulomatous colitic. J. Pharmacol. Exp. Ther. 271. 1114-1121
15 Takano, H., H.B. Lim, Y. Miyabara, T. Ichinose, T. Yoshikawa, and M. Sagai(1998b) Oral administration of L arginine potentiates allergen-induced inflammation and expression of interleukin-5 in mice. J. Phannacol. Exp, Ther. 286(2), 767-771
16 Bames, P.J. and F.Y. Liew (1995) Nitric oxide and athmalic inaammation. Immunol. today, 16(3), 128-30
17 Misko, T.P., W.M. Moore, T.P. Kasten, G.A. Nockols, J.A. Corbett, R.G. Tilton, M.L. McDaniel, J.R. Williamson, and M.G. Currie(1993) Selective inhibitionof the inducible nitric oxide synthase by amino-guanidine, Eur. J. Phannacol. 233, 119-125
18 Ichinose, T., Y. Yajima, M. Nagashima, S. Takenoshita, Y. Nagamachi, and Sagai, M. (1997) Lung carcinogenesis and formation of S-hydroxydeoxyguanosine in mice by diesel exhaust particles. Carcinogenesis 18, 185-192, 1997
19 Kharitonov, S.A., D.H. Yates. R.A. Robbins, R. Logan-Sinclair, E. Shineboume, and P.J. Barnes (1994) Increased nitric oxide in exhaled air of asthmatic patients. Lancet, 343, 133-135
20 Guo, H., J. Wei, and P.C. Kuo (2001) Nitric oxide inhibits expression of cytochrome B in endotoxin-stimulated murin macrophage. Biochem. Biophys. Res. Conunun. 289, 993-997
21 Wright, D.T.. L.A. Cohn, H. Li. B, Fischer, C.M. Li, and K.B. Adler(1994) Interactions of oxygen radicals with airway epithelium. Environ Health Perspect. 102(Suppl 10), 85-90
22 Hogman, M., C.G. Frostell, H. Hederiitrom, and G. Hedenstiema(1993) Inhalation of nitric oxide mo-dulates adult human bronchial tone. Am. Rev. Respir. Dis. 148, 1474-1478
23 Oshiba, A., E. Hamelmann, K. Takeda, K.L. Bradley. J.E. Loader, G.L. Larsen, and E.W. Gelfand (1996) Passive transfer of immediate hypersensitivity and airway hyperresponsiveness by allergen-specific immunoglobulin (IG) E and IgGl in mice. J. Clin. Invest. 5, 1398-1408
24 Persson, M.G., O. Zetterstrom, V. Argenius, E. Ihre, and L.E. Gustafsson(1993) Single-breath nitric oxide measurements in asthmatic patients and smokers. Lan-cet, 343, 146-147
25 Sagai, M., A. Furuyama, and T. Ichinose(1996) Biological effects of diesel exhauste particles (DEP). Ill. Pathogenesis of asthma like symptoms in mice. FreeRad. Biol. Med. 21(2), 199-209
26 Tomassini, M.A.. P.C. Tsicopoulos, V. Tai, A.B. Gruart, L. Tonnel, A. Prin, and M. Capron (1991) Release of granule proteins by eosinophils from allergic and nonallergic patients with eosinophilia on immuno-globulin-dependent activation. J. Allergy Clin. Immunol. 88, 365-375
27 Wu, C.C., S.J. Chen. C. Szabo, C. Thiemermann, and J.R. Vane (1995) Aminoguanidine attenuates the delayed circulatory failure and improves survival in ro-dent models of endotoxic shock. Br. J. Phannacol. 114, 1666-1672
28 Lim, H.B., T. Ichinose, Y. Miyabara, H. Takano, N. Shinoiyo,J. Devalia, and M. Sagai(1998) An involvement of superoxide and nitric oxide on airway inflammation and hyperresponsiveness induced by diesel exhaste particles in mice. Free. Radic. Biol. Med. 25(6), 635-644
29 Carroll, N., C. Cooke, and A. James (1997) The distribution of eosinophils and lymphocytes in the large and small airways of asthmatics. Eur. Respir. J. 10, 292-300
30 Miyabara, Y., R. Yanagisawa, N. Shimojo, H. Takano. H.B. Lim, T. Ichinose, and M. Sagai(1998a) Murinestrain differences in airway inflammation caused by diesel exhaust particles. Eur. Respir. J. 11. 291-298
31 Beckman, J.S., T.W. Beckman, J. Chen, P.A. Marshall. and B.A. Freeman(1990) Apparent hydroxyl radical production by peroxynitrite : Implications for endothelial injury from nitric oxide and superoxide. Proc. Natl. Acad. Sci. 87, 1620-1624
32 Sagai, M., H.B. Lim, and T. lchinose (2000) Lung carcinogenesis by diesel exhaust particles and the carcino-genie mechanism via active oxygens. Inhal. Toxicol. 12, 215-223
33 Sagai, M., H. Saito, T. Ichinose. M. Komada, and Y. Moh(1993): Biological effect of diesel exhaust particles. I. In vitro production production of superoxide and in vivo toxicity in mouse. Free Radic. Biol. Med. 14, 37-47