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The Relationship between Body Mass Index(BMI), Adipocyte Size and Leptin and Angiotensin II Secretion in Human Adipose Tissue  

Heo Young-Ran (Department of Food and Nutrition, Chonnam National University)
Moustaid-Moussa Naima (Department of Nutrition, University of Tennessee)
Publication Information
Journal of Community Nutrition / v.8, no.2, 2006 , pp. 69-75 More about this Journal
Abstract
Adipose tissue has now been recognized as a rich source of metabolically active molecules that include leptin and angiotensinogen (AGT), the precursor of angiotensin II (Ang II). Both of which have been implicated in the pathogenesis of metabolic alteration and hypertension associated with obesity. In this study, we examined the relationship between body mass index (BMI), adipocyte size, leptin, Ang II secretion and mRNA expression in human adipose tissue obtained from female subjects. Leptin and Ang II were analyzed using specific radioimmunoassay kits following a 48hour tissue culture. Leptin and Ang II secretion varied from 1.4 - 72.1ng/g and 0.8 - 57.3pg/g of tissue respectively. These large individual variations limit significant correlation between BMI, leptin and Ang II secretion. Ang II secretion was significantly higher in the obese than the non-obese (p < 0.05) and positively correlated with BMI. However, no difference in leptin secretion between the obese and the non-obese was observed and leptin secretion showed negative correlation with BMI. No difference in leptin and AGT mRNA expression in adipose tissue between the obese and the non-obese was observed. Although several limitations of this study, we found increased Ang II secretion in obese patients compared with non-obese patients, and positive correlation between AGT and BMI. Observed difference in AGT expression between the obese and the non-obese in this study might be of importance in relation with obesity related hypertension. (J Community Nutrition 8(2): 69-75, 2006)
Keywords
adipose tissue; angiotensin II; leptin; gene expression;
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1 Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, Ohannesian JP, Marco CC, McKee LJ, Bauer TL, Caro JF (1995): Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med 334: 292-295
2 Crandall DL, Armellino DC, Busler DE, mchendry-Rinde B, Kral JG (1999): Ang II receptors in human preadipocytes: role in cell cycle regulation. Endocrinol 140: 154-158   DOI   ScienceOn
3 Dusserre E, Moulin P, Vidal H (2000): Differences in mRNA expression of the proteins secreted by the adipocytes in human subcutaneous and visceral adipose tissues. Biochem Biophys Acta 1500: 88-96   DOI   ScienceOn
4 Engeli S, Negrel R, Sharma AM (2000): Physioloy and pathophysiology of the adipose tissue renin-angiotensin system. Hypertension 35: 1270-1277   DOI   ScienceOn
5 Faloia E, Gatti C, Camilloni MA, Mariniello B, Sardu C, Garrapa GG, Mantero F, Giacchetti G (2002) : Comparison of circulating' and local adipose tissue renin-angiotensin system in normotensive and hypertensive obese subjects. J Endocrinol Invest 25: 309-314   DOI
6 Faloia E, Giacchetti G, Mantero F (2000): Obesity and hypertension. J Enodocrinol Invest 23: 54-62   DOI
7 Giacchetti G, Faloia E, Mariniello B, Sardu C, Gatti C, Carnilloni MA, Guerrieri M, Mantero F (2002): Overexpression of the renin-angiotensin system in human visceral adipose tissue in normal and overweight subjects. Am J Hypertens 15: 381-388   DOI   ScienceOn
8 Harmelen V, Elizalde M, Ariapart P, Bergstedt-Lindqvist S, Reynis-dottir S, Hoffstedt J, Lundkvist I, Bringman S, Amer P (2000) : The association of human adipose angiotensinogen gene expression with abdominal fat distribution in obesity. Int J Obes Relat Metab Disord 24: 673-678   DOI   ScienceOn
9 Lonnqvist F, Arner P, Nordfores L, Schalling M (1995): Overexpression of obese (ob) gene in human obese subjects. Nat Med 1: 950-953   DOI
10 Montague CT, Prins JB, Sanders I, Digby JE, O'Rahilly S (1997): Depot and sex specific differences in human leptin mRNa expression: implications for the control of regional fat distribution. Diabetes 46: 342-347   DOI   ScienceOn
11 Prolo P, Wong ML, Licinio J (1998): Leptin. Int J Biochem Cell Biol 30: 1285-90   DOI   ScienceOn
12 Schorr D, Blaschke K, Turan S, Distler A, Sharma AM (1998): Relationship between angiotensinogen, leptin and blood pressure levels in young normotensive men. J Hypertens 16: 1475-1480   DOI   ScienceOn
13 Sharma AM, Engeli S, Pischon T (2001): New developments in mechanisms of obesity-induced hypertension: role of adipose tissue. Current Hypertension Reports 3: 152-156   DOI   ScienceOn
14 Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S, Kern PA, Friedman JM (1995): Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects Nat Med 1: 1155-1161   DOI
15 Faloia E, Carnilloni MA, Giacchetti G, Mantero F (2000): Adipose tissue as an endocrine organ? A review of some recent data. Eating & Weight Disorders 5: 116-123   DOI
16 Forrester T, McFarlane-Anderson N, Bennet F, Wilks R, Puras A, Cooper R, Rotimi C, Durazo R, Tewksbury D, Morrison L (1996): Angiotensinogen and blood pressure among blacks: findings from a community survey in Jamaica. J Hypertens 14: 315-321   DOI
17 Frederich RC, Hamann A, Anderson S, Lollmann B, Lowell BB, Flier JS (1995): Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action. Nat Med 1: 1311-1314   DOI
18 Caro JF, Sinha MK, Kolaczynski JW, Zhang PL, Considine RV (1996): Leptin: the tale of an obesity gene. Diabetes 45: 14551462   DOI
19 Fain JN, Cowan GS, Buffington C, Anderson RN, Pouncey L, Bahouth SW (2000): Regulation of leptin release by troglitazone in human adipose tissue. Metabolism 49: 1485-1490   DOI   ScienceOn
20 Bloem LJ, Manatunga AK, Tewksbury DA, Pratt JH (1995): The serum angiotensinogen concentration and various angiotensinogen genes in white and black children. J Clin Invest 95: 948-953   DOI   ScienceOn
21 Jones BH, Standridge MK, Moustaid N (1997): Angiotensin II increase lipogenesis in 3T3-L1 and human adipose tissue cells. Endocrinol 138: 1512-1519   DOI   ScienceOn
22 Giacchetti G, Faloia E, Sardu C, Carnilloni MA, Marinirllo B, Garrapa GGM, Guerrieri M, Mantero F (2000): Gene expression of angiotensinogen in adipose tissue of obese patients. Int J Obest 24: s142-s143   DOI
23 Hamilton BS, Palia D, Kwan AY, Deitel M (1995): Increased obese mRNA expression in omental fat cells from massively obese humans. Nat Med 1: 953-956   DOI
24 Mohamed-Ali V, Pinkney JH, Coppack SW (1998) : Adipose tissue as an endocrine and paracrine organ. Int J Obes Relat Metab Disord 22: 1145-1158   DOI   ScienceOn
25 Moustaid N, Jones B, Taylor JW (1996): Insulin increases lipogenic enzyme activity in human adipocytes in primary culture. J Nutr 126: 887-894   DOI
26 Umemura S, Nyui N, Tamura K, Hibi K, Yamaguchi S, Nakamaru M, Ishgami T, Yabana M, Kihara M, Inoue S, IshIi M (1997): Plasma angiotensinogen concentrations in obese patients. Am J Hypertens 10: 629-633   DOI   ScienceOn
27 Casabiell X, Pineiro V, Peino R, Lage M, Camina J, Gallego R, Vallejo LG, Dieguez C, Casanueva FF (1998): Gender differences in both spontaneous and stimulated leptin secretion by human omental adipose tissue in vitro: dexamethason and es-tradiol stimulate leptin release in women, but not in men. J Clin Endocrinol Metab 83: 2149-2155   DOI
28 Tamura K, Umemura S, Nyui N, Yamakawa T, Yamaguchi S, Ishigami T, Tanaka S, Tanimoto K, Takagi N, Sekihara H, Murakami K, Ishii M (1996): Tissue specific regulation of angiotensinogen gene expression in spontaneously hypertensive rats. Hypertens 27: 1216-1223   DOI   ScienceOn
29 Lonnqvist F, Nordfors L, Jansson M, Thome A, Schalling M, Arner P (1997): Leptin secretion from adipose tissue in women. J Clin Invest 99: 2398-2404   DOI   ScienceOn
30 Rodbell M (1964): Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239: 375-380
31 Menendez C, Baldelli R, Lage M, Casabiell X, Pinero V, Solar J, Dieguez C, Casanueva FF(2000): The in vitro secretion of human leptin is gender-dependent but independent of the body mass index of the donors. Eur J Endocrinol 143; 711-714   DOI
32 Saint-Marc P, Kozak LP, Ailhaud G, Darimont C, Negrel R (2001): Angiotensin II as a trophic factor of white adipose tissue: stimulation of adipose cell formation. Endocrinol 142: 487-492   DOI   ScienceOn
33 Havel PJ, Kasim-Karakas S, Mueller W, Johnson PR, Gingerich RL, Stern JS(1996): Relationship of plasma leptin to plasma insulin and adiposity in normal weight and overweight women: effects of dietary fat content and sustained weight loss. J Clin Endocrinol Metab 81: 4406-4413   DOI
34 Engeli S, Gorzeliniak K, Kreutz R, Runkel N, Distler A, Shrma AM (1999): Coexpression of renin-angiosystem genes in human adipose tissue. J Hypertens 17: 555-560   DOI   ScienceOn
35 Kim S, Whelan J, Claycombe K, Reath DB, Moustaid-Moussa N (2002): Angiotensin II increases leptin secretion by 3T3-Ll and human adipocytes via a prostaglandin-independent mechanism. J Nutr 132: 1135-1140   DOI