Browse > Article
http://dx.doi.org/10.1080/19768354.2011.577807

Regulatory expression and cellular localization of doublecortin in the rat retina following ischemia-reperfusion injury  

Gwon, Jae-Sung (Department of Anatomy, College of Medicine, The Catholic University of Korea)
Chun, Myung-Hoon (Department of Anatomy, College of Medicine, The Catholic University of Korea)
Kang, Wha-Sun (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
Publication Information
Animal cells and systems / v.15, no.2, 2011 , pp. 155-159 More about this Journal
Abstract
Doublecortin (DCX) is microtubule-associated protein and is required for neuronal migration, differentiation and plasticity. In the retina, it is highly expressed between embryonic day 18 (E18) and E20, and is poorly expressed postnatally. In this study, we investigated the expression and cellular localization of DCX in the rat retina following ischemia induced by transiently increasing the intraocular pressure. While DCX immunoreactivity in control retinas was restricted to the outer border of the inner nuclear layer, it appeared in horizontal cell somata and processes in affected retinas. Quantitative evaluation by immunoblotting confirmed that DCX expression continuously increased after ischemia-reperfusion and showed 370% of control protein levels at 4 weeks after ischemic insult. These results suggest that the DCX in horizontal cells might play a role in neurite remodeling or modulating other neurons in ischemic rat retinas.
Keywords
doublecortin; calbindin; horizontal cell; ischemia; rat; retina;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Sobel A. 1991. Stathmin: a relay phosphoprotein for multiple signal transductions. Trends Biochem Sci. 16:301-305.   DOI
2 Sossey-Alaoui K, Hartung AJ, Manchester DK, Posar A, Puche-Mira A, Andermann E, Dobyns WB, Srivastava AK. 1998. Human doublecortin (DCX) and the homologous gene in mouse encode a putative $Ca^{2+}$- dependent signaling protein which is mutated in human X-linked neuronal migration defect. Human Mol Genet. 8:1327-1333.
3 Tonchev AB, Yamashima T, Zhao L, Okano HJ, Okano H. 2003. Proliferation of neural and neuronal progenitors after global brain ischemia in young adult macaque monkeys. Mol Cell Neurosci. 23:292-301.   DOI   ScienceOn
4 Wada K, Sugimori H, Bhide PG, Moskowitz MA, Finklestein SP. 2003. Effect of basic fibroblast growth factor treatment on brain progenitor cells after permanent focal ischemia in rats. Stroke. 34:2722-2728.   DOI   ScienceOn
5 Ware ML, Fox JW, Gonzalez JL, Davis NM, Lambert de Rouvroit C, Russo CJ, Chua SC, Goffinet AM, Walsh CA. 1997. Aberrant splicing of a mouse disabled homolog, mdab1, in the scrambler mouse. Neuron. 19:1-20.   DOI   ScienceOn
6 Weiler R, Schultz K, Janssen-bienhold U. 1996. $Ca^{2+}$- dependency of spinule plasticity at dendrites of retinal horizontal cells and its possible implication for the functional role of spinules. Vision Res. 36:3891-3900.   DOI   ScienceOn
7 Williamson T, Gordon-Weeks PR, Schachner M, Taylor J. 1996. Microtubule reorganization is obligatory for growth cone turning. Proc Natl Acad Sci USA. 93:15221-15226.   DOI   ScienceOn
8 Brandstatter JH, Koulen P, Wassle H. 1998. Diversity of glutamate receptors in the mammalian retina. Vision Res. 38:1385-1397.   DOI   ScienceOn
9 Choi DW. 1994. Calcium and excitotoxic neuronal injury. Ann N Y Acad Sci. 747:162-171.
10 Choi DW. 1995. Calcium: still center-stage in hypoxic ischemic neuronal death. Trends Neurosci. 18:58-60.   DOI   ScienceOn
11 Chun MH, Kim IB, Ju WK, Kim KY, Joo CK, Chung JW. 1999. Horizontal cells of the rat retina are resistant to degenerative processes induced by ischemiareperfusion. Neurosci Lett. 260:125-128.   DOI   ScienceOn
12 Francis F, Koulakoff A, Boucher D, Chafey P, Schaar B, Vinet M, Friocourt G, McDonnell N, Reiner O, Kahn A, McConnell SK, Berwald-Netter Y, Denoulet P, Chelly J. 1999. Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons. Neuron. 23:247-256.   DOI   ScienceOn
13 Gleeson JG, Lin PT, Flanagan LA, Walsh CA. 1999. Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons. Neuron. 23:257-271.   DOI   ScienceOn
14 Gordon-Weeks PR. 1991. Evidence for microtubule capture by filopodial actin filaments in growth cones. Neuroreport. 2:573-576.   DOI   ScienceOn
15 Ju WK, Kim KY, Hofmann HD, Kim IB, Lee MY, Oh SJ, Chun MH. 2000. Selective neuronal survival and upregulation of PCNA in the rat inner retina following transient ischemia. J Neuropathol Exp Neurol. 59:241-250.   DOI
16 Hannan AJ, Henke RC, Seeto GS, Capes-Davis A, Dunn J, Jeffrey PL. 1999. Expression of doublecortin correlates with neuronal migration and pattern formation in diverse regions of the developing chick brain. J Neurosci Res. 55:650-657.   DOI   ScienceOn
17 Jin K, Minami M, Lan JQ, Mao XO, Batterur S, Simon RP, Greenberg DA. 2001. Neurogenesis in dentate subgranular zone and rostal subventricular zone after focal cerebral ischemia in the rat. Proc Natl Acad Sci USA. 98:4710-4715.   DOI   ScienceOn
18 Jin K, Sun Y, Xie L, Peel A, Mao XO, Batteur S, Greenberg DA. 2003. Directed migration of neuronal precursors into the ischemic cerebral cortex and striatum. Mol Cell Neurosci. 24:171-189.   DOI   ScienceOn
19 Kim IB, Kim KY, Joo CK, Lee MY, Oh SJ, Chung JW, Chun MH. 1998. Reaction of Muller cells after increased intraocular pressure in the rat retina. Exp Brain Res. 121:419-424.   DOI   ScienceOn
20 Lee EJ, Kim IB, Lee E, Kwon SO, Oh SJ, Chun MH. 2003. Differential expression and cellular localization of doublecortin in the developing rat retina. Eur J Neurosci. 17:1542-1548.   DOI   ScienceOn
21 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem. 193:265-275.
22 Nacher J, Crespo C, McEwen BS. 2001. Doublecortin expression in the adult rat telencephalon. Eur J Neurosci. 14:629-644.   DOI   ScienceOn
23 Ochiishi T, Terashima T, Sugiura H, Yamauchi T. 1994. Immunohistochemical localization of $Ca^{2+}$/calmodulindependent protein kinase II in the rat retina. Brain Res. 634:257-265.   DOI   ScienceOn
24 Peterson GL. 1979. Review of the folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall. Anal Biochem. 100:201-220.   DOI   ScienceOn
25 Rohrenbeck J, Wassle H, Heizmann CW. 1987. Immunocytochemical labelling of horizontal cells in mammalian retina using antibodies against calcium-binding proteins. Neurosci Lett. 77:255-260.   DOI   ScienceOn
26 Siesjo BK, Smith ML. 1991. The biochemical basis of ischemic brain lesions. Arzneimittelforschung. 41:288-292.