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http://dx.doi.org/10.1080/19768354.2010.525824

Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression  

Jung, Woo-Sang (Department of Cardiovascular and Neurologic Diseases, Hospital of Oriental Medicine, Kyung Hee Medical Center, Kyung Hee University)
Cho, Jin-Gu (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
In, Kyung-Min (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
Kim, Jong-Min (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
Cho, Ki-Ho (Department of Cardiovascular and Neurologic Diseases, Hospital of Oriental Medicine, Kyung Hee Medical Center, Kyung Hee University)
Park, Jung-Mi (Department of Stroke and Neurological Disorders Center, East-West Neo Medical Center, Kyung Hee University)
Moon, Sang-Kwan (Department of Cardiovascular and Neurologic Diseases, Hospital of Oriental Medicine, Kyung Hee Medical Center, Kyung Hee University)
Kim, Kyung-Wook (Department of Cardiovascular and Neurologic Diseases, Hospital of Oriental Medicine, Kyung Hee Medical Center, Kyung Hee University)
Park, Seong-Uk (Department of Stroke and Neurological Disorders Center, East-West Neo Medical Center, Kyung Hee University)
Pyee, Jae-Ho (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
Park, Sang-Gyu (Department of Biomedical Science, CHA University)
Jeong, Yoon-Hwa (Department of Food Science and Nutrition, Dankook University)
Park, Heon-Yong (Department of Molecular Biology & Institute of Nanosensor and Biotechnology, BK21 Graduate Program for RNA Biology, Dankook University)
Ko, Chang-Nam (Department of Stroke and Neurological Disorders Center, East-West Neo Medical Center, Kyung Hee University)
Publication Information
Animal cells and systems / v.14, no.4, 2010 , pp. 275-282 More about this Journal
Abstract
Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1 (VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1 leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from downregulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1 and VCAM-1 gene expression.
Keywords
chunghyul-dan; chemotaxis; cell adhesion; endothelial cells; transmigration;
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1 Navab M, Imes SS, Hama SY, Hough GP, Ross LA, Bork RW, Valente AJ, Berliner JA, Drinkwater DC, Laks. 1991. Monocyte transmigration induced by modification of low density lipoprotein in cocultures of human aortic wall cells is due to induction of monocyte chemotactic protein 1 synthesis and is abolished by high density lipoprotein. J Clin Invest. 88:2039-2046.   DOI
2 Park H, Go YM, St John PL, Maland MC, Lisanti MP, Abrahamson DR, Jo H. 1998. Plasma membrane cholesterol is a key molecule in shear stress-dependent activation of extracellular signal-regulated kinase. J Biol Chem. 273:32304-32311.   DOI   ScienceOn
3 Park SG, Kang YS, Ahn YH, Lee SH, Kim KR, Kim KW, Koh GY, Ko YG, Kim SH. 2002. Dose-dependent biphasic activity of tRNA synthetase-associating factor, p43, in angiogenesis. J Biol Chem. 277:45243-45248.   DOI   ScienceOn
4 Park SU, Jung WS, Moon SK, Ko CN, Cho KH, Kim YS, Bae HS. 2006. Chunghyul-dan (Qingxie-dan) improves arterial stiffness in patients with increased baPWV. Am J Chin Med. 34:553-563.   DOI   ScienceOn
5 Ardigo D, Assimes TL, Fortmann SP, Go AS, Hlatky M, Hytopoulos E, Iribarren C, Tsao PS, Tabibiazar TR. 2007. Quertermous; ADVANCE Investigators. Circulating chemokines accurately identify individuals with clinically significant atherosclerotic heart disease. Physiol Genomics. 31:402-409.   DOI   ScienceOn
6 Charo IF, Taubman MB. 2004. Chemokines in the pathogenesis of vascular disease. Circ Res. 95:858-866.   DOI   ScienceOn
7 Dattner AM. 2003. From medical herbalism to phytotherapy in dermatology: back to the future. Dermatol Ther. 16:106-113.   DOI   ScienceOn
8 Davies PF. 1995. Flow-mediated endothelial mechanotransduction. Physiol Rev. 75:519-560.   DOI
9 Ramos CL, Huo Y, Jung U, Ghosh S, Manka DR, Sarembock IJ, Ley K. 1999. Direct demonstration of P-selectin- and VCAM-1-dependent mononuclear cell rolling in early atherosclerotic lesions of apolipoprotein E-deficient mice. Circ Res. 84:1237-1244.   DOI   ScienceOn
10 Schepers A, Eefting D, Bonta PI, Grimbergen JM, de Vries MR, vanWeel V, de vries CJ, Egashira K, van Bockel JH, Quax PH. 2006. Anti-MCP-1 gene therapy inhibits vascular smooth muscle cells proliferation and attenuates vein graft thickening both in vitro and in vivo. Arterioscler Thromb Vasc Biol. 26:2063-2069.   DOI   ScienceOn
11 Sheikine Y, Hansson GK. 2004. Chemokines and atherosclerosis. Ann Med. 36:98-118.
12 Shin J, Kim J, Ryu B, Chi S, Park H. 2006. Caveolin-1 is associated with VCAM-1 dependent adhesion of gastric cancer cells to endothelial cells. Cell Physiol Biochem. 17:211-220.   DOI   ScienceOn
13 Stary HC, Chandler AB, Dinsmore RE, Fuster V, Glagov S, Insull W Jr, Rosenfeld ME, Schwartz CJ, Wagner WD, Wissler RW. 1995. A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. Circulation. 92:1355-1374.   DOI   ScienceOn
14 Gupta H, Dai L, Datta G, Garber DW, Grenett H, Li Y, Mishra V, Palqunachari MN, Handattu S, Gianturco SH, Bradley WA, Anantharamaiah GM, White CR. 2005. Inhibition of lipopolysaccharide-induced inflammatory responses by an apolipoprotein AI mimetic peptide. Circ Res. 97:236-243.   DOI   ScienceOn
15 Ikeda U. 2003. Inflammation and coronary artery disease. Curr Vasc Pharmacol. 1:65-70.   DOI   ScienceOn
16 Eriksson EE, Werr J, Guo Y, Thoren P, Lindbom L. 2000. Direct observations in vivo on the role of endothelial selectins and alpha(4) integrin in cytokine-induced leukocyte-endothelium interactions in the mouse aorta. Circ Res. 86:526-533.   DOI   ScienceOn
17 Eriksson EE, Xie X, Werr J, Thoren P, Lindbom L. 2001. Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins. FASEB J. 15:1149-1157.   DOI
18 Jo H, Sipos K, Go YM, Law R, Rong J, McDonald JM. 1997. Differential effect of shear stress on extracellular signal-regulated kinase and N-terminal Jun kinase in endothelial cells. Gi2- and Gbeta/gamma-dependent signaling pathways. J Biol Chem. 272:1395-1401.   DOI   ScienceOn
19 Weiss JM, Nath A, Major EO, Berman JW. 1999. HIV-1 Tat induces monocyte chemoattractant protein-1-mediated monocyte transmigration across a model of the human blood-brain barrier and up-regulates CCR5 expression on human monocytes. J Immunol. 163:2953-2959.
20 Suenaga T, Shirakawa T, Harada, Tao C, Yamasaki M, Jomatsu H, Matsumoto N, Kida M, Kawamoto Y, Murakami Y. 1991. Mechanism of protective effects of ouren-gedoku-to and san'ou-syashin-to on the gastric mucosa. Nippon Yakurigaku Zasshi. 98:319-325.   DOI   ScienceOn
21 Karin M, Lawrence T, Nizet V. 2006. Innate immunity gone awry: linking microbial infections to chronic inflammation and cancer. Cell. 124:823-835.   DOI   ScienceOn
22 Kim YS, Jung EA, Shin JI, Chan JC, Yang HK, Kim NJ, Cho KH, Bae HS, Moon SK, Kim DH. 2002. Daio-Orengedokuto inhibits HMG-CoA reductase and pancreatic lipase. Biol Pharm Bull. 25:1442-1445.   DOI   ScienceOn
23 Masedunskas A, King JA, Tan F, Cochran R, Stevens T, Sviriov D, Ofori-Acquah SF. 2006. Activated leukocyte cell adhesion molecule is a component of the endothelial junction involved in transendothelial monocyte migration. FEBS Lett. 580:2637-2645.   DOI   ScienceOn
24 Mi H, Guo N, Kejariwal A, Thomas PD. 2007. PANTHER version 6: protein sequence and function evolution data with expanded representation of biological pathways. Nucleic Acids Res. 35:D247-D252.   DOI   ScienceOn
25 Cho KH, Jung WS, Park SU, Moon SK, Ko CN, Ku S, Chi SG, Park H. 2004. Daio-Orengedokuto works as a cellproliferating compound in endothelial cells. Can J Physiol Pharmacol. 82:380-386.   DOI   ScienceOn
26 Mahalinagam S, Karupiah G. 1999. Chemokines and chemokine recpetors in infectious diseases. Immunol Cell Biol. 77:469-475.   DOI
27 Sertic J, V XJ, Bozina N, Malencia B, Kes P, Reiner Z. 2007. Cytokines and growth factors in mostly atherosclerotic patients on hemodialysis determined by biochip array technology. Clin Chem Lab Med. 45:1347-1352.   DOI   ScienceOn
28 Thomas PD, Campbell MJ, Kejariwal A, Mi H, Karlak B, Daverman R, Diemer, Muruganujan A, Narechania A. 2003. PANTHER: a library of protein families and subfamilies indexed by function. Genome Res. 13:2129-2141.   DOI   ScienceOn