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http://dx.doi.org/10.1080/19768354.2010.528204

Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins  

Heo, Jee-In (Department of Biochemistry, College of Medicine, Hallym University)
Oh, Soo-Jin (Department of Biochemistry, College of Medicine, Hallym University)
Kho, Yoon-Jung (Institute of Natural Medicine, College of Medicine, Hallym University)
Kim, Jeong-Hyeon (Department of Biochemistry, College of Medicine, Hallym University)
Kang, Hong-Joon (Department of Biochemistry, College of Medicine, Hallym University)
Park, Seong-Hoon (Molecular Radiation Oncology, Radiation Oncology Branch, Center for Cancer Research, NCI, NIH)
Kim, Hyun-Seok (Molecular Radiation Oncology, Radiation Oncology Branch, Center for Cancer Research, NCI, NIH)
Shin, Jong-Yeon (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Lee, Sung-Young (Department of Biochemistry, College of Medicine, Hallym University)
Kim, Min-Ju (Department of Anatomy and Neurobiology, College of Medicine, Hallym University)
Min, Bon-Hong (Department of Pharmacology, College of Medicine, Korea University)
Kim, Sung-Chan (Department of Biochemistry, College of Medicine, Hallym University)
Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University)
Kim, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University)
Lee, Jae-Yong (Department of Biochemistry, College of Medicine, Hallym University)
Publication Information
Animal cells and systems / v.14, no.4, 2010 , pp. 261-266 More about this Journal
Abstract
PC12 cells were differentiated into the cells of chromaffin phenotype by butyrate treatment. Cells were aggregated and formed tight cell adhesion. To investigate the molecular change in this differentiation, we examined expression levels of cell adhesion proteins and extracellular proteins during butyrate induced-differentiation of PC12 cells. Integrin ${\beta}1$, integrin ${\alpha}7$, E cadherin, VCAM, collagen-I, fibronectin, desmoglein and connexin were increased during differentiation. The levels of clusterin and secreted clusterin were also increased. These increased levels of cell adhesion proteins and extracellular proteins appear to induce cell aggregation and tight cell adhesion. The levels of p21, p27 and p16 were increased probably because of differentiation-related growth arrest during differentiation. Prolonged incubation of butyrate up to 1 day was required for differentiation. Signal transduction pathways for this differentiatiom could not be identified since various inhibitors had no effect. The results showed that butyrateinduced differentiation of PC12 cells to chromaffin cells involves tight cell adhesion and induction of extracellular proteins and cell adhesion proteins.
Keywords
butyrate; PC12; differentiation; chromaffin cells; cell adhesion proteins;
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1 Greene LA, Tischler AS. 1976. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA. 73:2424-2428.   DOI   ScienceOn
2 Gumbiner BM. 1996. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell. 84:345-357.   DOI   ScienceOn
3 Jones SE, Jomary C. 2002. Clusterin. Int J Biochem Cell Biol. 34:427-431.   DOI   ScienceOn
4 Kruh J. 1982. Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem. 42:65-82.
5 Lee DY, Hayes JJ, Pruss D, Wolffe AP. 1993. A positive role for histone acetylation in transcription factor access to nucleosomal DNA. Cell. 72:73-84.   DOI   ScienceOn
6 Makrilia N, Kollias A, Manolopoulos L, Syrigos K. 2009. Cell adhesion molecules: role and clinical significance in cancer. Cancer Invest. 27:1023-1037.   DOI   ScienceOn
7 Meighan CM, Schwarzbauer JE. 2008. Temporal and spatial regulation of integrins during development. Curr Opin Cell Biol. 20:520-524.   DOI   ScienceOn
8 Nuutinen T, Suuronen T, Kyrylenko S, Huuskonen J, Salminen A. 2005. Induction of clusterin/apoJ expression by histone deacetylase inhibitors in neural cells. Neurochem Int. 47:528-538.   DOI   ScienceOn
9 Nuutinen T, Suuronen T, Kauppinen A, Salminen A. 2009. Clusterin: a forgotten player in Alzheimer's disease. Brain Res Rev. 61:89-104.   DOI   ScienceOn
10 Richon VM, Sandhoff TW, Rifkind RA, Marks PA. 2000. Histone deacetylase inhibitor selectively induces p21WAF1 expression and gene-associated histone acetylation. Proc Natl Acad Sci USA. 97:10014-10019.   DOI   ScienceOn
11 Vettese-Dadey M, Grant PA, Hebbes TR, Crane-Robinson C, Allis CD, Workman JL. 1996. Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro. Embo J. 15:2508-2518.
12 Byrd JC, Naranjo JR, Lindberg I. 1987. Proenkephalin gene expression in the PC12 pheochromocytoma cell line: stimulation by sodium butyrate. Endocrinology. 121:1299-1305.   DOI   ScienceOn
13 Allfrey VG, Faulkner R, Mirsky AE. 1964. Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc Natl Acad Sci U S A. 51:786-794.   DOI   ScienceOn
14 Byrd JC, Alho H. 1987. Differentiation of PC12 pheochromocytoma cells by sodium butyrate. Brain Res. 428:151-155.
15 Byrd JC, Lichti U. 1987. Two types of transglutaminase in the PC12 pheochromocytoma cell line. Stimulation by sodium butyrate. J Biol Chem. 262:11699-11705.
16 Davie JR. 2003. Inhibition of histone deacetylase activity by butyrate. J Nutr. 133:2485S-2493S.   DOI
17 Fujita K, Lazarovici P, Guroff G. 1989. Regulation of the differentiation of PC12 pheochromocytoma cells. Environ Health Perspect. 80:127-142.   DOI   ScienceOn
18 Paschos KA, Canovas D, Bird NC. 2009. The role of cell adhesion molecules in the progression of colorectal cancer and the development of liver metastasis. Cell Signal. 21:665-674.   DOI   ScienceOn