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http://dx.doi.org/10.1080/19768351003770871

Identification of differentially expressed genes in the developmental stages from olive flounder Paralichthys olivaceus using an annealing control primer system  

Kim, Young-Ok (Biotechnology Research Division, National Fisheries Research and Development Institute)
Park, Eun-Mi (Biotechnology Research Division, National Fisheries Research and Development Institute)
Nam, Bo-Hye (Biotechnology Research Division, National Fisheries Research and Development Institute)
Kong, Hee-Jeong (Biotechnology Research Division, National Fisheries Research and Development Institute)
Kim, Woo-Jin (Biotechnology Research Division, National Fisheries Research and Development Institute)
Noh, Jae-Koo (Fish Genetics and Breeding Research Center, National Fisheries Research and Development Institute)
Lee, Sang-Jun (Biotechnology Research Division, National Fisheries Research and Development Institute)
Kim, Kyung-Kil (Biotechnology Research Division, National Fisheries Research and Development Institute)
Publication Information
Animal cells and systems / v.14, no.1, 2010 , pp. 25-30 More about this Journal
Abstract
We employed a new and improved differential display reverse transcription-polymerase chain reaction (DDRT-PCR) method, which involves annealing control primers (ACPs), to identify the genes that are specifically or prominently expressed in olive flounder (Paralichthys olivaceus) juveniles (35 days post-hatch; dph) compared to larval-stage (dph 21) flounder. Using 60 ACPs, we identified eight differentially expressed genes (DEGs) and basic local alignment search tool (BLAST) searches revealed eight known genes. Gene expression levels were confirmed by RT-PCR. Phosphoglucose isomerase (PGI) was highly expressed at 21 dph, while nephrosin, myosin light chain (MLC), myosin heavy chain (MHC), carboxypeptidase A, chymotrypsin B, fish-egg protein, and matrix protein were expressed at 35 dph. PGI, MLC, and MHC expression was further analyzed by RT-PCR. The differentially expressed genes identified in this study may provide insights into the molecular basis of development in olive flounder.
Keywords
annealing control primer; gene expression; Paralichthys olivaceus; RT-PCR;
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