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Isolation of Caenorhabditis elegans Mutants Defective in Chemotaxis toward cAMP  

Jeong, Jin-A (School of Life Sciences, Chungbuk National University)
Cho, Nam-Jeong (School of Life Sciences, Chungbuk National University)
Publication Information
Animal cells and systems / v.10, no.4, 2006 , pp. 237-241 More about this Journal
Abstract
Chemotactic behavior is essential for the survival of animals. However, the mechanism by which animals carry out chemotaxis is poorly understood. To explore the biochemical events underlying chemotaxis, we isolated C. elegans mutants that displayed abnormal chemotactic responses to cAMP, a strong attractant for C. elegans. Based on their responses to other chemoattractants, the mutant animals could be classified into five groups: (1) animals with defective chemotaxis to cAMP only; (2) animals with defective chemotaxis to both cAMP and cGMP; (3) animals with defective chemotaxis to water-soluble attractants; (4) animals with defective chemotaxis to both water-soluble and volatile attractants; and (5) animals with enhanced chemotactic responses. We expect that analyses of these mutants will help understand the molecular mechanisms underlying chemotaxis in C. elegans.
Keywords
Chemotaxis; C. elegans; cAMP; animal behavior; mutant;
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1 Ward S (1973) Chemotaxis by the nematode Caenorhabditis elegans: identification of attractants and analysis of the response by use of mutants. Proc Natl A cad USA 70: 817-821   DOI   ScienceOn
2 White JG; Southgate E, Thomson IN, and Brenner S (1986) The structure of the nervous system of the nematode Caenorhabditis elegans. Phil Trans R Soc Lond B 314: 1-340   DOI   ScienceOn
3 Troemel ER, Chou JH, Dwyer ND, Colbert HA, and Bargmann CI (1995) Divergent seven transmembrane receptors are candidate chemosensory receptors in C. elegans. Cell 83 : 207-218   DOI   ScienceOn
4 Troemel ER, Kimmel BE, and Bargmann CI (1997) Reprogmmming chemotaxis responses: sensory neurons defme olfactory preferences in C. elegans. Cell 91: 161-169   DOI   ScienceOn
5 Sengupta P, Chou JH, and Bargmann CI (1996) odr-10 encodes a seven tmnsmembrane domain olfactory receptor required for responses to the odorant diacetyl. Cell 84: 899-909   DOI   ScienceOn
6 Strmecki L, Greene DM, and Pears CJ (2005) Developmental decisions in Dictyostelium discoideum. Dev Bioi 284: 25-36   DOI   ScienceOn
7 Dusenbery DB, Sheridan RE, and Russell RL (1975) Chemotaxis-defective mutants of the nematode Caenorhabditis elegans. Genetics 80: 297-309
8 Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, and Mello CC (1998) Potent and specific genetic interference by double-stmnded RNA in Caenorhabditis elegans. Nature 391: 806-811   DOI   ScienceOn
9 Jeong J, Hwang JM, and Cho NJ (1996) Chemosensation in Caenorhabditis elegans: identification of attractants and analysis of chemotactic behavior. Genet Eng Res 10: 1-15
10 Matsuki M, Kunitomo H, and lino Y (2006) Goa regulates olfactory adaptation by antagonizing Gqa-DAG signaling in Caenorhabditis elegans. Proc Natl Acad USA 103: 1112-1117   DOI   ScienceOn
11 Chalfie M, Tu Y, Euskirchen G; Ward WW, and Pmsher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805   DOI
12 Mori I and Ohshima Y (1997) Molecular neurogenetics of chemotaxis and thermotaxis in the nematode Caenorhabditis elegans. BioEssays 19: 1055-1064   DOI   ScienceOn
13 Bargmann CI (1998) Neurobiology of the Caenorhabditis elegans genome. Science 282: 2028-2033   DOI   ScienceOn
14 Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71-94
15 Colbert HA and Bargmann CI (1995) Odorant-specific adaptation pathways generate olfactory plasticity in C. elegans. Neuron 14: 803-812   DOI   ScienceOn
16 Bargmann CI (1993) Genetic and cellular analysis of behavior in C. elegans. Annu Rev Neurosci 16: 47-71   DOI   ScienceOn
17 Bargmann CI, Hartwieg E, and Horvitz HR (1993) Odorantselective genes and neurons mediate olfaction in C. elegans. Cell 74: 515-527   DOI   ScienceOn
18 Bargmann CI and Horvitz HR (1991) Chemosensory neurons with overlapping functions direct chemotaxis to multiple chemicals in C. elegans. Neuron 7: 729-742   DOI   ScienceOn