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Caspase-11 Promoter-GFP Construct as a Dual Reporter of Cytotoxicity and Inflammation  

Shin, Ki-Soon (Department of Biology, College of Sciences, Kyung Hee University)
Kang, Shin-Jung (Department of Molecular Biology, College of Natural Sciences, Sejong University)
Publication Information
Animal cells and systems / v.10, no.2, 2006 , pp. 73-77 More about this Journal
Abstract
Caspase-11 has been known as a dual regulator of apoptosis and inflammatory response. An unusual feature of caspase-11 is that its expression is induced by apoptotic or proinflammatory stimuli. Utilizing these unusual features of caspase-11, we have developed a simple and sensitive assay method to screen pro- or anti-apoptotic/inflammatory molecules. To develop this assay method, we generated a reporter construct where GFP expression is regulated by caspase-11 promoter. When several types of cultured cells were transfected with this reporter construct and subsequently treated with various apoptotic or proinflammatory molecules, expression of GFP by the activation of caspase-11 promoter was easily detected by fluorescence microscopy or spectrofluorometry. In addition, a reduction of the GFP fluorescence was detected when an agent reported to suppress caspase-11 induction was applied. These results suggest that our reporter system can be used to screen pro- or anti-apoptotic/inflammatory molecules.
Keywords
Caspase-11 promoter-GFP; cytotoxicity; inflammation;
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