Browse > Article

Genetic Approach to Identify Critical Factors for Mouse Early Embryogenesis  

Park, Joon-Hyun (Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University)
Kim, Ji-Soo (Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University)
Sonn, Sung-Keun (Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University)
Rhee, Kun-Soo (Department of Biological Sciences and Research Center for Functional Cellulomics, Seoul National University)
Publication Information
Animal cells and systems / v.10, no.1, 2006 , pp. 41-47 More about this Journal
Abstract
Development of the mammalian pre-implantation embryos has unique features, such as a slow and unsynchronized cell division, compaction, and eventual formation of blastocysts with inner cell mass and trophectoderm. In order to have a clue on molecular mechanisms that reside in mouse early development, we suppressed expression of early embryo-specific genes with RNAi and observed their development in vitro. We observed developmental defects in embryos microinjected with dsRNAs for Oct4 or Nanog among the tested genes. Careful examinations revealed that development of the most of the Oct4- or Nanog-suppressed embryos were arrested at the morula stage. These results suggest that the Oct4 and Nanog activities are also required for embryogenesis earlier than the blastocyst stage.
Keywords
Embryogenesis; Nanog; Oct4; Sox2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim MH, Yuan X, Okumura S, and Ishikawa F (2002) Successful inactivation of endogenous Oct-3/4 and c-mos genes in mouse preimplantation embryos and oocytes using short interfering RNAs. Biochem Biophys Res Commun 296: 1372-1377   DOI   ScienceOn
2 Chatot CL, Ziomek CA, Bavister BD, Lewis JL, and Torres I (1989) An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 86: 679-688   DOI   ScienceOn
3 Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, and Smith A (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113: 643-655   DOI   ScienceOn
4 Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, and Young RA (2005) Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122: 947-956   DOI   ScienceOn
5 Bortvin A, Eggan K, Skaletsky H, Akutsu H, Berry DL, Yanagimachi R, Page DC, and Jaenisch R (2003) Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei. Development 130: 1673-1680   DOI   ScienceOn
6 Aravind L, and Koonin EV (2000) SAP - a putative DNA-binding motif involved in chromosomal organization. Trends Biochem Sei 25: 112-114   DOI   ScienceOn
7 Sonn S, Khang I, Kim K, and Rhee K (2004) Suppression of Nek2A in mouse early embryos confirms its requirement for chromosome segregation. J Cell Sci 117: 5557-5566   DOI   ScienceOn
8 Amano H, Itakura K, Maruyama M, Ichisaka T, Nakagawa M, and Yamanaka S (2006) Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5). BMC Dev Biol 6: 11   DOI
9 van Baren N, Chambost H, Ferrant A, Michaux L, Ikeda H, Millard I, Olive D, Boon T, and Coulie PG (1998) PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells. Br J Haematol 102: 1376-1379   DOI   ScienceOn
10 Wianny F, and Zernicka-Goetz M (2000) Specific interference with gene function by double-stranded RNA in early mouse development. Nat Cell Biol 2: 70-75   DOI   ScienceOn
11 Takahashi K, Mitsui K, and Yamanaka S (2003) Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. Nature 423: 541-545   DOI   ScienceOn
12 Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, and Yamanaka S (2003) The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113: 631-642   DOI   ScienceOn
13 Siomi H, Choi M, Siomi MC, Nussbaum RL, and Dreyfuss G (1994) Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome. Cell 77: 33-39   DOI   ScienceOn
14 Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, and Smith A (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95: 379-391   DOI   ScienceOn
15 Shin MR, Cui XS, Jun JH, Jeong YJ, and Kim NH (2005) Identification of mouse blastocyst genes that are downregulated by double-stranded RNA-mediated knockdown of Oct-4 expression. Mol Reprod Dev 70: 390-396   DOI   ScienceOn
16 Nagy A, Gertsenstein M, Vintersten K, and Behringer R (2002) Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 289-358
17 Korioth F, Gieffers C, Maul GG, and Frey J (1995) Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment. J Cell Biol 130: 1-13   DOI
18 Ichimura T, Bonventre JV, Bailly V, Wei H, Hession CA, Cate RL, and Sanicola M (1998) Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J Biol Chem 273: 4135-4142   DOI   ScienceOn