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Tra2${\alpha}$ and hnRNP K might be Functional Partners of Rbm for Regulation of RNA Processes during Spermatogenesis  

Lee, Jungmin (School of Biological Sciences, Seoul National University)
Kim, Euisu (School of Biological Sciences, Seoul National University)
Jang, Sung Key (Department of Life Science, Pohang University of Science and Technology)
Rhee, Kunsoo (School of Biological Sciences, Seoul National University)
Publication Information
Animal cells and systems / v.8, no.1, 2004 , pp. 65-70 More about this Journal
Abstract
Rbm is a male infertility gene located in the AZFb region of the Y chromosome. Expression pattern of Rbm indicates that Rbm is critical for early phase of male germ cell development. It shares strong structural homology with hnRNP G, suggesting a function as an RNA processing factor. In order to gain a clue on the molecular mechanisms of Rbm on male germ cell development, we examined interactions of Rbm with selected proteins in yeast. The results revealed specific interactions between Rbm, hnRNP K and Tra2${\alpha}$. These results suggest that hnRNP K and Tra2${\alpha}$ may be functional partners of Rbm in male germ cells. We propose a model in which hnRNP K may playa role as a platform for Rbm and Tra2${\alpha}$.
Keywords
Male infertility; Rbm; hnRNP K; Tra2${\alpha}$; Spermatogenesis;
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1 Matsuo N, Ogawa S, Imai Y, Takagi T, Tohyama M, Stern D, and Wanaka A (1995) Cloning of a novel RNA binding polypeptide (RA301) induced by hypoxia/reoxygenation. J Biol Chem 270: 28216-28222   DOI
2 Mattox W and Baker BS (1991) Autoregulation of the splicing of transcripts from the transformer-2 gene of Drosophila. Genes Dev 5: 786-796   DOI   ScienceOn
3 Mahadevaiah SK, Odorisio T, Elliott DJ, Rattigan A. Szot M, Laval SH, Washburn LL, McCarrey JR, Cattanach BM, Lovell-Badge R, and Burgoyne PS (1998) Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 7: 715-727   DOI
4 Ma K, Inglis JD, Sharkey A, Bickmore WA, Hill RE, Prosser EJ, Speed RM, Thomson EJ, Jobling M, Taylor K, et al. (1993) A Y chromosome gene family with RNA-binding protein homology: candidates for the azoospermia factor AZF controlling human spermatogenesis. Cell 75: 1287-1295   DOI   ScienceOn
5 Lee JM, Hong JP, Kim ES, Kim K, Kim SW, Krishnamurthy H. Chung SSW, Wolgemuth DJ, and Rhee K (2004) Developmental stage-specific expression of Rbm suggests its involvement in early phases of spermatogenesis. Mol Human Reprod: in press   DOI
6 Lynch KW and Maniatis T (1995) Synergistic interactions between two distinct elements of a regulated splicing enhancer. Genes Dev 9: 284-293   DOI   ScienceOn
7 Ito K, Sato K, and Endo H (1994) Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch. Nucleic Acids Res 22: 53-58   DOI   ScienceOn
8 Kim JH, Hahm B, Kim YK, Choi M, and Jang SK (2000) Protein-protein interaction among hnRNPs shuttling between nucleus and cytoplasm. J Mol Biol 298: 395-405   DOI   ScienceOn
9 Hobert O, Jallal B, Schlessinger J, and Ullrich A (1994) Novel signaling pathway suggested by SH3 domain-mediated p95vav/heterogeneous ribonucleoprotein K interaction. J Biol Chem 269: 20225-20228
10 Fields S and Song O (1989) A novel genetic system to detect protein-protein interactions. Nature 340: 245-246   DOI   ScienceOn
11 Elliott DJ, Bourgeois CF, Klink A, Stevenin J, and Cooke HJ (2000) Mammalian germ cell-specific RNA-binding protein interacts with ubiquitously expressed proteins involved in splice site selection. Proc Natl Acad Sci USA 97: 5717-5722   DOI
12 Foresta C, Moro E, and Ferlin A (2001) Y chromosome microdeletions and alterations of spermatogenesis. Endocr Rev 22: 226-239   DOI   ScienceOn
13 Expert-Bezancon A, Le Caer JP, and Marie J (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem 277: 16614-16623   DOI   ScienceOn
14 Feilotter HE, Hannon GJ, Ruddell CJ, and Beach D (1994) Construction of an improved host strain for two hybrid screening. Nucleic Acids Res 22: 1502-1503   DOI
15 Denisenko O and Bomsztyk K (2002) Yeast hnRNP K-like genes are involved in regulation of the telomeric position effect and telomere length. Mol Cell Biol 22: 286-297   DOI
16 Delbridge ML, Lingenfelter PA, Disteche CM, and Graves JA (1999) The candidate spermatogenesis gene RBMY has a homologue on the human X chromosome. Nat Genet 22: 223-224   DOI   ScienceOn
17 Denisenko ON, O'Neill B, Ostrowski J, Van Seuningen I, and Bomsztyk K (1996) Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein. J Biol Chem 271: 27701-27706   DOI   ScienceOn
18 Du Q, Melnikova IN, and Gardner PD (1998) Differential effects of heterogeneous nuclear ribonucleoprotein K on Sp1- and Sp3-mediated transcriptional activation of a neuronal nicotinic acetylcholine receptor promoter. J Biol Chem 273: 19877-19883   DOI
19 Dauwalder B, Amaya-Manzanares F, and Mattox W (1996) A human homologue of the Drosophila sex determination factor transformer-2 has conserved splicing regulatory functions. Proc Natl Acad Sci 93: 9004-9009   DOI
20 Andersson M, Page DC, Pettay D, Subrt I, Turleau C, de Grouchy J, and de la Chapelle A (1988) Y; autosome translocations and mosaicism in the aetiology of 45, X maleness: assignment of fertility factor to distal Yq11. Hum Mol Genet 79: 2-7   DOI
21 Beil B, Screaton G, and Stamm S (1997) Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. DNA Cell Biol 16: 679-690
22 Bomsztyk K, Van Seuningen I, Suzuki H, Denisenko O, and Ostrowski J (1997) Diverse molecular interactions of the hnRNP K protein. FEBS Lett 403: 113-115   DOI   ScienceOn
23 Tiepolo L and Zuffardi O (1976) Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Hum Mol Genet 34: 119-124   DOI
24 Bustelo XR, Suen KL, Michael WM, Dreyfuss G, and Barbacid M (1995) Association of the vav proto-oncogene product with poly(rC)-specific RNA-binding proteins. Mol Cell Biol 15: 1324-1332   DOI
25 Vergnaud G, Page DC, Simmler MC, Brown L, Rouyer F, Noel B, Botstein D, de la Chapelle A, and Weissenbach J (1986) A deletion map of the human Y chromosome based on DNA hybridization. Am J Hum Genet 38: 109-124
26 Wadd S, Bryant H, Filhol O, Scott JE, Hsieh TY, Everett RD, and Clements JB (1999) The multifunctional herpes simplex virus IE63 protein interacts with heterogeneous ribonucleoprotein K and with casein kinase 2. J Biol Chem 274: 28991-28998   DOI
27 Venables JP, Elliott DJ, Makarova OV, Makarov EM, Cooke HJ, and Eperon IC (2000) RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with $Tra2{\beta}$ and affect splicing. Hum Mol Genet 9: 685-694   DOI   ScienceOn
28 Seong JY, Han J, Park S, Wuttke W, Jarry H, and Kim K (2002) Exonic splicing enhancer-dependent splicing of the gonadotropin-releasing hormone premessenger ribonucleic acid is mediated by $tra2{\alpha}$, a 40-kilodalton serine/arginine-rich protein. Mol Endocrinol 16: 2426-2438   DOI   ScienceOn
29 Swanson MS and Dreyfuss G (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol 8: 2237-2241   DOI
30 Tacke R, Tohyama M, Ogawa S, and Manley JL (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell 93: 139-148   DOI   ScienceOn
31 van Seuningen I, Ostrowski J, Bustelo XR, Sleath PR, and Bomsztyk K (1995) The K protein domain that recruits the interleukin 1-responsive K protein kinase lies adjacent to a cluster of c-Src and Vav SH3-binding sites. Implications that K protein acts as a docking platform. J Biol Chem 270: 26976-26985   DOI   ScienceOn
32 Ostrowski J, Van Seuningen I, Seger R, Rauch CT, Sleath PR, McMullen BA, and Bomsztyk K (1994) Purification, cloning, and expression of a murine phosphoprotein that binds the kappa B motif in vitro identifies it as the homolog of the human heterogeneous nuclear ribonucleoprotein K protein. Description of a novel DNA-dependent phosphorylation process. J Biol Chem 269: 17626-17634
33 Schullery DS, Ostrowski J, Denisenko ON, Stempka L, Shnyreva M, Suzuki H, Gschwendt M, and Bomsztyk K (1999) Regulated interaction of protein kinase Cdelta with the heterogeneous nuclear ribonucleoprotein K protein. J Biol Chem 274: 15101-15109   DOI   ScienceOn
34 Segade F, Hurle B, Claudio E, Ramos S, and Lazo PS (1996) Molecular cloning of a mouse homologue for the Drosophila splicing regulator Tra2. FEBS Lett 387: 152-156   DOI   ScienceOn
35 Ostrowski J, Schullery DS, Denisenko ON, Higaki Y, Watts J, Aebersold R, Stempka L, Gschwendt M, and Bomsztyk K (2000) Role of tyrosine phosphorylation in the regulation of the interaction of heterogenous nuclear ribonucleoprotein K protein with its protein and RNA partners. J Biol Chem 275: 3619-3628   DOI   ScienceOn
36 Ostareck-Lederer A, Ostareck DH, Cans C, Neubauer G, Bomsztyk K, Superti-Furga G, and Hentze MW (2002) c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs. Mol Cell Biol 22: 4535-4543   DOI
37 Ostareck-Lederer A, Ostareck DH, and Hentze MW (1998) Cytoplasmic regulatory functions of the KH-domain proteins hnRNPs K and E1/E2. Trends Biochem Sci 23: 409-411   DOI   ScienceOn
38 Michelotti EF, Michelotti GA, Aronsohn AI, and Levens D (1996) Heterogeneous nuclear ribonucleoprotein K is a transcription factor. Mol Cell Biol 16: 2350-2360   DOI
39 Ostrowski J, Kawata Y, Schullery DS, Denisenko ON, Higaki Y, Abrass CK, and Bomsztyk K (2001) Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA. Proc Natl Acad Sci 98: 9044-9049   DOI   ScienceOn
40 Michael WM, Eder PS, and Dreyfuss G (1997) The K nuclear shuttling domain: a novel signal for nuclear import and nuclear export in the hnRNP K protein. EMBO J 16: 3587-3598   DOI   ScienceOn
41 Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, and Hentze MW (1997) mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end. Cell 89: 597-606   DOI   ScienceOn
42 Mazeyrat S, Saut N, Mattei MG, and Mitchell MJ (1999) RBMY evolved on the Y chromosome from a ubiquitously transcribed X-Y identical gene. Nat Genet 22: 224-226   DOI   ScienceOn
43 Mattox W, Palmer MJ, and Baker BS (1990) Alternative splicing of the sex determination gene transformer-2 is sex-specific in the germ line but not in the soma. Genes Dev 4: 789-805   DOI   ScienceOn
44 Matunis MJ, Michael WM, and Dreyfuss G (1992) Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein. Mol Cell Biol 12: 164-171   DOI