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http://dx.doi.org/10.14348/molcells.2020.0098

CXCR4-STAT3 Axis Plays a Role in Tumor Cell Infiltration in an Orthotopic Mouse Glioblastoma Model  

Han, Ji-hun (Department of Biomedical Sciences, Ajou Graduate School)
Yoon, Jeong Seon (Department of Anatomy, Ajou University School of Medicine)
Chang, Da-Young (Department of Biomedical Sciences, Ajou Graduate School)
Cho, Kyung Gi (Department of Neurosurgery, Bundang CHA Medical Center, CHA University College of Medicine)
Lim, Jaejoon (Department of Neurosurgery, Bundang CHA Medical Center, CHA University College of Medicine)
Kim, Sung-Soo (Department of Biomedical Sciences, Ajou Graduate School)
Suh-Kim, Haeyoung (Department of Biomedical Sciences, Ajou Graduate School)
Publication Information
Molecules and Cells / v.43, no.6, 2020 , pp. 539-550 More about this Journal
Abstract
Glioblastoma multiforme (GBM) is a fatal malignant tumor that is characterized by diffusive growth of tumor cells into the surrounding brain parenchyma. However, the diffusive nature of GBM and its relationship with the tumor microenvironment (TME) is still unknown. Here, we investigated the interactions of GBM with the surrounding microenvironment in orthotopic xenograft animal models using two human glioma cell lines, U87 and LN229. The GBM cells in our model showed different features on the aspects of cell growth rate during their development, dispersive nature of glioma tumor cells along blood vessels, and invasion into the brain parenchyma. Our results indicated that these differences in the two models are in part due to differences in the expression of CXCR4 and STAT3, both of which play an important role in tumor progression. In addition, the GBM shows considerable accumulation of resident microglia and peripheral macrophages, but polarizes differently into tumor-supporting cells. These results suggest that the intrinsic factors of GBM and their interaction with the TME determine the diffusive nature and probably the responsiveness to non-cancer cells in the TME.
Keywords
glioblastoma; invasion; LN229; tumor microenvironment; U87;
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