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http://dx.doi.org/10.14348/molcells.2020.2231

DNAJB9 Inhibits p53-Dependent Oncogene-Induced Senescence and Induces Cell Transformation  

Lee, Hyeon Ju (Department of Biochemistry and Molecular Biology, Kangwon National University School of Medicine)
Jung, Yu-Jin (Department of Biological Sciences, Kangwon National University)
Lee, Seungkoo (Department of Anatomic Pathology, Kangwon National University School of Medicine, Kangwon National University Hospital)
Kim, Jong-Il (Genomic Medicine Institute, Medical Research Center, Seoul National University)
Han, Jeong A. (Department of Biochemistry and Molecular Biology, Kangwon National University School of Medicine)
Publication Information
Molecules and Cells / v.43, no.4, 2020 , pp. 397-407 More about this Journal
Abstract
DNAJB9 is known to be a member of the molecular chaperone gene family, whose cellular function has not yet been fully characterized. Here, we investigated the cellular function of DNAJB9 under strong mitogenic signals. We found that DNAJB9 inhibits p53-dependent oncogene-induced senescence (OIS) and induces neoplastic transformation under oncogenic RAS activation in mouse primary fibroblasts. In addition, we observed that DNAJB9 interacts physically with p53 under oncogenic RAS activation and that the p53-interacting region of DNAJB9 is critical for the inhibition of p53-dependent OIS and induction of neoplastic transformation by DNAJB9. These results suggest that DNAJB9 induces cell transformation under strong mitogenic signals, which is attributable to the inhibition of p53-dependent OIS by physical interactions with p53. This study might contribute to our understanding of the cellular function of DNAJB9 and the molecular basis of cell transformation.
Keywords
DNAJB9; p53; RAS; senescence; transformation;
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