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http://dx.doi.org/10.14348/molcells.2018.0056

Tectorigenin Promotes Osteoblast Differentiation and in vivo Bone Healing, but Suppresses Osteoclast Differentiation and in vivo Bone Resorption  

Lee, So-Youn (Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University)
Kim, Gyu-Tae (Department of Oral and Maxillofacial Radiology, School of Dentistry, Kyung Hee University)
Yun, Hyung-Mun (Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University)
Kim, Youn-Chul (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Kwon, Il- Keun (Department of Dental Materials, School of Dentistry, Kyung Hee University)
Kim, Eun-Cheol (Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University)
Publication Information
Molecules and Cells / v.41, no.5, 2018 , pp. 476-485 More about this Journal
Abstract
Although tectorigenin (TG), a major compound in the rhizome of Belamcanda chinensis, is conventionally used for the treatment of inflammatory diseases, its effects on osteogenesis and osteoclastogenesis have not been reported. The objective of this study was to investigate the effects and possible underlying mechanism of TG on in vitro osteoblastic differentiation and in vivo bone formation, as well as in vitro osteoclast differentiation and in vivo bone resorption. TG promoted the osteogenic differentiation of primary osteoblasts and periodontal ligament cells. Moreover, TG upregulated the expression of the BMP2, BMP4, and Smad-4 genes, and enhanced the expression of Runx2 and Osterix. In vivo studies involving mouse calvarial bone defects with ${\mu}CT$ and histologic analysis revealed that TG significantly increased new bone formation. Furthermore, TG treatment inhibited osteoclast differentiation and the mRNA levels of osteoclast markers. In vivo studies of mice demonstrated that TG caused the marked attenuation of bone resorption. These results collectively demonstrated that TG stimulated osteogenic differentiation in vitro, increased in vivo bone regeneration, inhibited osteoclast differentiation in vitro, and suppressed inflammatory bone loss in vivo. These novel findings suggest that TG may be useful for bone regeneration and treatment of bone diseases.
Keywords
bone remodeling; differentiation; osteoblast; osteoclast; tectorigenin;
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