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http://dx.doi.org/10.14348/molcells.2017.0081

Modulation of Mitochondrial Membrane Potential and ROS Generation by Nicotinamide in a Manner Independent of SIRT1 and Mitophagy  

Song, Seon Beom (Department of Life Science, University of Seoul)
Jang, So-Young (Department of Life Science, University of Seoul)
Kang, Hyun Tae (Department of Life Science, University of Seoul)
Wei, Bie (Department of Life Science, University of Seoul)
Jeoun, Un-woo (Department of Biomedical Science and Department of Biochemistry, Ajou University School of Medicine)
Yoon, Gye Soon (Department of Biomedical Science and Department of Biochemistry, Ajou University School of Medicine)
Hwang, Eun Seong (Department of Life Science, University of Seoul)
Publication Information
Molecules and Cells / v.40, no.7, 2017 , pp. 503-514 More about this Journal
Abstract
Nicotinamide (NAM) plays essential roles in physiology through facilitating $NAD^+$ redox homeostasis. Importantly, at high doses, it protects cells under oxidative stresses, and has shown therapeutic effectiveness in a variety of disease conditions. In our previous studies, NAM lowered reactive oxygen species (ROS) levels and extended cellular life span in primary human cells. In the treated cells, levels of $NAD^+/NADH$ and SIRT1 activity increased, while mitochondrial content decreased through autophagy activation. The remaining mitochondria were marked with low superoxide levels and high membrane potentials (${\Delta}_{{\Psi}m}$); we posited that the treatment of NAM induced an activation of mitophagy that is selective for depolarized mitochondria, which produce high levels of ROS. However, evidence for the selective mitophagy that is mediated by SIRT1 has never been provided. This study sought to explain the mechanisms by which NAM lowers ROS levels and increases ${\Delta}_{{\Psi}m}$. Our results showed that NAM and SIRT1 activation exert quite different effects on mitochondrial physiology. Furthermore, the changes in ROS and ${\Delta}_{{\Psi}m}$ were not found to be mediated through autophagy or SIRT activation. Rather, NAM suppressed superoxide generation via a direct reduction of electron transport, and increased ${\Delta}_{{\Psi}m}$ via suppression of mitochondrial permeability transition pore formation. Our results dissected the effects of cellular $NAD^+$ redox modulation, and emphasized the importance of the $NAD^+/NADH$ ratio in the mitochondria as well as the cytosol in maintaining mitochondrial quality.
Keywords
membrane potential; mitochondria; mitophagy; mPTP; nicotinamide; SIRT1; superoxide;
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