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http://dx.doi.org/10.14348/molcells.2017.2298

Assessment of the Therapeutic Potential of Persimmon Leaf Extract on Prediabetic Subjects  

Khan, Mohd M. (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland)
Tran, Bao Quoc (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland)
Jang, Yoon-Jin (Department of Pharmacology, Chonbuk National University Medical School)
Park, Soo-Hyun (Clinical Trial Center for Functional Foods, Chonbuk National University Hospital)
Fondrie, William E. (University of Maryland School of Medicine)
Chowdhury, Khadiza (University of Maryland School of Medicine)
Yoon, Sung Hwan (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland)
Goodlett, David R. (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland)
Chae, Soo-Wan (Department of Pharmacology, Chonbuk National University Medical School)
Chae, Han-Jung (Department of Pharmacology, Chonbuk National University Medical School)
Seo, Seung-Young (Department of Internal Medicine, Research Institute of Clinical Medicine, Chonbuk National University Medical School and Hospital)
Goo, Young Ah (Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland)
Publication Information
Molecules and Cells / v.40, no.7, 2017 , pp. 466-475 More about this Journal
Abstract
Dietary supplements have exhibited myriads of positive health effects on human health conditions and with the advent of new technological advances, including in the fields of proteomics, genomics, and metabolomics, biological and pharmacological activities of dietary supplements are being evaluated for their ameliorative effects in human ailments. Recent interests in understanding and discovering the molecular targets of phytochemical-gene-protein-metabolite dynamics resulted in discovery of a few protein signature candidates that could potentially be used to assess the effects of dietary supplements on human health. Persimmon (Diospyros kaki) is a folk medicine, commonly used as dietary supplement in China, Japan, and South Korea, owing to its different beneficial health effects including anti-diabetic implications. However, neither mechanism of action nor molecular biomarkers have been discovered that could either validate or be used to evaluate effects of persimmon on human health. In present study, Mass Spectrometry (MS)-based proteomic studies were accomplished to discover proteomic molecular signatures that could be used to understand therapeutic potentials of persimmon leaf extract (PLE) in diabetes amelioration. Saliva, serum, and urine samples were analyzed and we propose that salivary proteins can be used for evaluating treatment effectiveness and in improving patient compliance. The present discovery proteomics study demonstrates that salivary proteomic profile changes were found as a result of PLE treatment in prediabetic subjects that could specifically be used as potential protein signature candidates.
Keywords
biomarker candidates; diabetes; mass spectrometry; persimmon leaf extract; proteomics; saliva; serum; urine;
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