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http://dx.doi.org/10.14348/molcells.2017.2320

Neuroprotective Effects of Protein Tyrosine Phosphatase 1B Inhibition against ER Stress-Induced Toxicity  

Jeon, Yu-Mi (Department of Neural Development and Disease, Korea Brain Research Institute (KBRI))
Lee, Shinrye (Department of Neural Development and Disease, Korea Brain Research Institute (KBRI))
Kim, Seyeon (Department of Neural Development and Disease, Korea Brain Research Institute (KBRI))
Kwon, Younghwi (Department of Neural Development and Disease, Korea Brain Research Institute (KBRI))
Kim, Kiyoung (Department of Medical Biotechnology, Soonchunhyang University)
Chung, Chang Geon (Department of Brain & Cognitive Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Lee, Seongsoo (Gwangju Center, Korea Basic Science Institute (KBSI))
Lee, Sung Bae (Department of Brain & Cognitive Sciences, Daegu Gyeongbuk Institute of Science and Technology (DGIST))
Kim, Hyung-Jun (Department of Neural Development and Disease, Korea Brain Research Institute (KBRI))
Publication Information
Molecules and Cells / v.40, no.4, 2017 , pp. 280-290 More about this Journal
Abstract
Several lines of evidence suggest that endoplasmic reticulum (ER) stress plays a critical role in the pathogenesis of many neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. Protein tyrosine phosphatase 1B (PTP1B) is known to regulate the ER stress signaling pathway, but its role in neuronal systems in terms of ER stress remains largely unknown. Here, we showed that rotenone-induced toxicity in human neuroblastoma cell lines and mouse primary cortical neurons was ameliorated by PTP1B inhibition. Moreover, the increase in the level of ER stress markers ($eIF2{\alpha}$ phosphorylation and PERK phosphorylation) induced by rotenone treatment was obviously suppressed by concomitant PTP1B inhibition. However, the rotenone-induced production of reactive oxygen species (ROS) was not affected by PTP1B inhibition, suggesting that the neuroprotective effect of the PTP1B inhibitor is not associated with ROS production. Moreover, we found that MG132-induced toxicity involving proteasome inhibition was also ameliorated by PTP1B inhibition in a human neuroblastoma cell line and mouse primary cortical neurons. Consistently, downregulation of the PTP1B homologue gene in Drosophila mitigated rotenone- and MG132-induced toxicity. Taken together, these findings indicate that PTP1B inhibition may represent a novel therapeutic approach for ER stress-mediated neurodegenerative diseases.
Keywords
endoplasmic reticulum stress (ER Stress); MG132; reactive oxygen species (ROS); rotenone; ubiquitin proteasome system;
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