[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.14348/molcells.2016.0149

DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex

Jang, Soo Hwa (School of Biological Sciences, University of Ulsan)
Kim, Ah-Ram (School of Biological Sciences, University of Ulsan)
Park, Neung-Hwa (Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital)
Park, Jeong Woo (School of Biological Sciences, University of Ulsan)
Han, In-Seob (School of Biological Sciences, University of Ulsan)

Abstract

Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [ $^3H$ ] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but upregulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.

Keywords

cyclin B/Cdk1 complex; DRG2; G2/M check point; p21;

Citations & Related Records

Reference

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