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http://dx.doi.org/10.14348/molcells.2016.0112

LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium  

Lee, Eun-Ju (Department of Biochemistry, College of Medicine, Chungbuk National University)
Park, Kwan-Sik (Department of Biochemistry, College of Medicine, Chungbuk National University)
Jeon, In-Sook (Department of Biochemistry, College of Medicine, Chungbuk National University)
Cho, Jae-Woon (Department of Surgery, College of Medicine, Chungbuk National University)
Lee, Sang-Jeon (Department of Surgery, College of Medicine, Chungbuk National University)
Choy, Hyun E. (Department of Microbiology, Chonnam National University Medical School)
Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National University)
Lee, Hak-Kyo (Department of Animal Biotechnology, Chonbuk National University)
Choi, Joong-Kook (Department of Biochemistry, College of Medicine, Chungbuk National University)
Publication Information
Molecules and Cells / v.39, no.7, 2016 , pp. 566-572 More about this Journal
Abstract
Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella- induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.
Keywords
LAMP-3; LPS; lysosome; Salmonella typhimurium;
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