Browse > Article
http://dx.doi.org/10.14348/molcells.2016.2170

Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats  

Wang, Haidong (College of Food and Biological Engineering, Hubei University of Technology)
Li, Deyuan (Nutrition and Food Research Institute, Wuhan Economic College)
Hu, Zhongze (Nutrition and Food Research Institute, Wuhan Economic College)
Zhao, Siming (College of Food Science and Technology, Huazhong Agricultural University)
Zheng, Zhejun (Nutrition and Food Research Institute, Wuhan Economic College)
Li, Wei (College of Food and Biological Engineering, Hubei University of Technology)
Publication Information
Molecules and Cells / v.39, no.6, 2016 , pp. 508-513 More about this Journal
Abstract
To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-1-${\beta}$ (IL-1-${\beta}$) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb induced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols.
Keywords
lead; oxidative stress; polyphenols; ROS; renal inflammation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bohn, T. (2014). Dietary factors affecting polyphenol bioavailability. Nutr. Rev. 72, 429-452.   DOI
2 Cardona, F., Andres-Lacueva, C., Tulipani, S., Tinahones, F.J., and Queipo-Ortuno, M.I. (2013). Benefits of polyphenols on gut microbiota and implications in human health. J. Nutr. Biochem. 24, 1415-1422.   DOI
3 Chandran, L., and Cataldo, R. (2010). Lead poisoning: basics and new developments. P Pediatr Rev. 31, 399-405; quiz 406.   DOI
4 Chu, A.J. (2014). Antagonism by bioactive polyphenols against inflammation: a systematic view. Inflamm. Allergy Drug Targets 13, 34-64.   DOI
5 Copello, G.J., Pesenti, M.P., Raineri, M., Mebert, A.M., Piehl, L.L., de Celis, E.R., and Diaz, L.E. (2013). Polyphenol-SiO2 hybrid biosorbent for heavy metal removal. Yerba mate waste (Ilex paraguariensis) as polyphenol source: kinetics and isotherm studies. Colloids Surf. B. Biointerfaces 102, 218-226.   DOI
6 Dai, W., Du, H., Fu, L., Jin, C., Xu, Z., and Liu, H. (2009). Effects of dietary Pb on accumulation, histopathology, and digestive enzyme activities in the digestive system of tilapia (Oreochromis niloticus). Biol. Trace Elem. Res. 127, 124-131.   DOI
7 Doumouchtsis, K.K., Doumouchtsis, S.K., Doumouchtsis, E.K., and Perrea, D.N. (2009). The effect of lead intoxication on endocrine functions. J. Endocrinol. Invest. 32, 175-183.   DOI
8 Garza, A., Vega, R., and Soto, E. (2006). Cellular mechanisms of lead neurotoxicity. Med. Sci. Monit. 12, RA57-65.
9 Joven, J., Micol, V., Segura-Carretero, A., Alonso-Villaverde, C., Menendez, J.A., and Bioactive Food Components, P. (2014). Polyphenols and the modulation of gene expression pathways: can we eat our way out of the danger of chronic disease? Crit. Rev. Food Sci. Nutr. 54, 985-1001.   DOI
10 Khurana, S., Venkataraman, K., Hollingsworth, A., Piche, M., and Tai, T.C. (2013). Polyphenols: benefits to the cardiovascular system in health and in aging. Nutrients 5, 3779-3827.   DOI
11 Kusumoto, M., Kamobayashi, H., Sato, D., Komori, M., Yoshimura, M., Hamada, A., Kohda, Y., Tomita, K., and Saito, H. (2011). Alleviation of cisplatin-induced acute kidney injury using phytochemical polyphenols is accompanied by reduced accumulation of indoxyl sulfate in rats. Clin. Exp. Nephrol. 15, 820-830.   DOI
12 Liu, C.M., Sun, Y.Z., Sun, J.M., Ma, J.Q., and Cheng, C. (2012). Protective role of quercetin against lead-induced inflammatory response in rat kidney through the ROS-mediated MAPKs and NFkappaB pathway. Biochim. Biophys. Acta 1820, 1693-1703.   DOI
13 Li, C., Xu, M., Wang, S., Yang, X., Zhou, S., Zhang, J., Liu, Q., and Sun, Y. (2011). Lead exposure suppressed ALAD transcription by increasing methylation level of the promoter CpG islands. Toxicol. Lett. 203, 48-53.   DOI
14 Li, Y.W., Zhang, Y., Zhang, L., Li, X., Yu, J.B., Zhang, H.T., Tan, B.B., Jiang, L.H., Wang, Y.X., Liang, Y., et al. (2014). Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-kappaB p65 signal pathway. Gene 542, 46-51.   DOI
15 Liu, M.Y., Hsieh, W.C., and Yang, B.C. (2000). In vitro aberrant gene expression as the indicator of lead-induced neurotoxicity in U-373MG cells. Toxicology 147, 59-64.   DOI
16 Luo, W., Ruan, D., Yan, C., Yin, S., and Chen, J. (2012). Effects of chronic lead exposure on functions of nervous system in Chinese children and developmental rats. Neurotoxicology 33, 862-871.   DOI
17 Luo, M., Xu, Y., Cai, R., Tang, Y., Ge, M.M., Liu, Z.H., Xu, L., Hu, F., Ruan, D.Y., and Wang, H.L. (2014). Epigenetic histone modification regulates developmental lead exposure induced hyperactivity in rats. Toxicol. Lett. 225, 78-85.   DOI
18 Mandal, S., Mukherjee, S., Chowdhury, K.D., Sarkar, A., Basu, K., Paul, S., Karmakar, D., Chatterjee, M., Biswas, T., Sadhukhan, G.C., et al. (2012). S-allyl cysteine in combination with clotrimazole downregulates Fas induced apoptotic events in erythrocytes of mice exposed to lead. Biochim. Biophys. Acta 1820, 9-23.   DOI
19 Mishra, K.P., Singh, V.K., Rani, R., Yadav, V.S., Chandran, V., Srivastava, S.P., and Seth, P.K. (2003). Effect of lead exposure on the immune response of some occupationally exposed individuals. Toxicology 188, 251-259.   DOI
20 Mason, L.H., Harp, J.P., and Han, D.Y. (2014). Pb neurotoxicity: neuropsychological effects of lead toxicity. BioMed Res. Int. 2014, 840547.
21 Murakami, A. (2014). Dose-dependent functionality and toxicity of green tea polyphenols in experimental rodents. Arch. Biochem. Biophys. 557, 3-10.   DOI
22 Navarro-Moreno, L.G., Quintanar-Escorza, M.A., Gonzalez, S., Mondragon, R., Cerbon-Solorzano, J., Valdes, J., and Calderon-Salinas, J.V. (2009). Effects of lead intoxication on intercellular junctions and biochemical alterations of the renal proximal tubule cells. Toxicol. In Vitro 23, 1298-1304.   DOI
23 Nemsadze, K., Sanikidze, T., Ratiani, L., Gabunia, L., and Sharashenidze, T. (2009). Mechanisms of lead-induced poisoning. Georgian Med. News 92-96.
24 Ohara, M., and Ohyama, Y. (2014). Delivery and application of dietary polyphenols to target organs, tissues and intracellular organelles. Curr. Drug Metabol. 15, 37-47.   DOI
25 Poreba, R., Gac, P., Poreba, M., and Andrzejak, R. (2012). Assessment of cardiovascular risk in workers occupationally exposed to lead without clinical presentation of cardiac involvement. Envir. Toxicol. Pharmacol. 34, 351-357.   DOI
26 Qiao, J., Kong, X., Kong, A., and Han, M. (2014). Pharmacokinetics and biotransformation of tea polyphenols. Curr. Drug Metabol. 15, 30-36.   DOI
27 Shinkai, Y., and Kaji, T. (2012). Cellular defense mechanisms against lead toxicity in the vascular system. Biol. Pharm. Bull. 35, 1885-1891.   DOI
28 Warniment, C., Tsang, K., and Galazka, S.S. (2010). Lead poisoning in children. Am. Fam. Physician 81, 751-757.
29 Struzynska, L., Dabrowska-Bouta, B., Koza, K., and Sulkowski, G. (2007). Inflammation-like glial response in lead-exposed immature rat brain. Toxicol. Sci. 95, 156-162.   DOI
30 van der Kuijp, T.J., Huang, L., and Cherry, C.R. (2013). Health hazards of China's lead-acid battery industry: a review of its market drivers, production processes, and health impacts. Environ. Health 12, 61.   DOI
31 Yang, Y.S., Wang, C.J., Huang, C.N., Chen, M.L., Chen, M.J., and Peng, C.H. (2013). Polyphenols of Hibiscus sabdariffa improved diabetic nephropathy via attenuating renal epithelial mesenchymal transition. J. Agric. Food Chem. 61, 7545-7551.   DOI
32 Yang, P., He, X., and Malhotra, A. (2014). Epigenetic targets of polyphenols in cancer. J Environ Pathol Toxicol Oncol. 33, 159-165.   DOI
33 Ye, X., and Wong, O. (2006). Lead exposure, lead poisoning, and lead regulatory standards in China, 1990-2005. Regul. Toxicol. Pharmacol. 46, 157-162.   DOI
34 Zou, D., and Xie, A. (2013). Influence of polyphenol-plasma protein interaction on the antioxidant properties of polyphenols. Curr. Drug Metabol. 14, 451-455.   DOI