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http://dx.doi.org/10.14348/molcells.2016.0088

Clearing and Labeling Techniques for Large-Scale Biological Tissues  

Seo, Jinyoung (Department of Chemistry, Seoul National University)
Choe, Minjin (Department of Biophysics and Chemical Biology, Seoul National University)
Kim, Sung-Yon (Department of Chemistry, Seoul National University)
Publication Information
Molecules and Cells / v.39, no.6, 2016 , pp. 439-446 More about this Journal
Abstract
Clearing and labeling techniques for large-scale biological tissues enable simultaneous extraction of molecular and structural information with minimal disassembly of the sample, facilitating the integration of molecular, cellular and systems biology across different scales. Recent years have witnessed an explosive increase in the number of such methods and their applications, reflecting heightened interest in organ-wide clearing and labeling across many fields of biology and medicine. In this review, we provide an overview and comparison of existing clearing and labeling techniques and discuss challenges and opportunities in the investigations of large-scale biological systems.
Keywords
3D volume imaging; CLARITY; large-scale tissue clearing; stochastic electrotransport; SWITCH; whole-mount labeling;
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