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http://dx.doi.org/10.14348/molcells.2016.0144

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors  

Zhang, Guo Hua (College of Life Science and Engineering, Northwest University for Nationalities)
Lu, Jian Xiong (College of Life Science and Engineering, Northwest University for Nationalities)
Chen, Yan (College of Life Science and Engineering, Northwest University for Nationalities)
Dai, Hong Wei (College of Life Science and Engineering, Northwest University for Nationalities)
ZhaXi, YingPai (College of Life Science and Engineering, Northwest University for Nationalities)
Zhao, Yong Qing (Gansu Engineering Research Center for Animal Cell, Northwest University for Nationalities)
Qiao, Zi Lin (Gansu Engineering Research Center for Animal Cell, Northwest University for Nationalities)
Feng, Ruo Fei (College of Life Science and Engineering, Northwest University for Nationalities)
Wang, Ya Ling (College of Life Science and Engineering, Northwest University for Nationalities)
Ma, Zhong Ren (Gansu Engineering Research Center for Animal Cell, Northwest University for Nationalities)
Publication Information
Molecules and Cells / v.39, no.11, 2016 , pp. 797-806 More about this Journal
Abstract
Lipogenesis is under the concerted action of ChREBP, SREBP-1c and other transcription factors in response to glucose and insulin. The isolated porcine preadipocytes were differentiated into mature adipocytes to investigate the roles and interrelation of these transcription factors in the context of glucose- and insulin-induced lipogenesis in pigs. In ChREBP-silenced adipocytes, glucose-induced lipogenesis decreased by ~70%, however insulin-induced lipogenesis was unaffected. Moreover, insulin had no effect on ChREBP expression of unperturbed adipocytes irrespective of glucose concentration, suggesting ChREBP mediate glucose-induced lipogenesis. Insulin stimulated SREBP-1c expression and when SREBP-1c activation was blocked, and the insulin-induced lipogenesis decreased by ~55%, suggesting SREBP-1c is a key transcription factor mediating insulin-induced lipogenesis. $LXR{\alpha}$ activation promoted lipogenesis and lipogenic genes expression. In ChREBP-silenced or SREBP-1c activation blocked adipocytes, $LXR{\alpha}$ activation facilitated lipogenesis and SREBP-1c expression, but had no effect on ChREBP expression. Therefore, $LXR{\alpha}$ might mediate lipogenesis via SREBP-1c rather than ChREBP. When ChREBP expression was silenced and SREBP-1c activation blocked simultaneously, glucose and insulin were still able to stimulated lipogenesis and lipogenic genes expression, and $LXR{\alpha}$ activation enhanced these effects, suggesting $LXR{\alpha}$ mediated directly glucose- and insulin-induced lipogenesis. In summary, glucose and insulin stimulated lipogenesis through both dissimilar and identical regulation pathway in porcine adipocytes.
Keywords
ChREBP; $LXR{\alpha}$; lipogenesis; porcine adipocytes; SREBP-1c;
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