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http://dx.doi.org/10.14348/molcells.2016.0183

Carboxypeptidase E, Identified As a Direct Interactor of Growth Hormone, Is Important for Efficient Secretion of the Hormone  

Mizutani, Akiko (Basic Medical Science and Molecular Medicine, Tokai University School of Medicine)
Inoko, Hidetoshi (Basic Medical Science and Molecular Medicine, Tokai University School of Medicine)
Tanaka, Masafumi (Basic Medical Science and Molecular Medicine, Tokai University School of Medicine)
Publication Information
Molecules and Cells / v.39, no.10, 2016 , pp. 756-761 More about this Journal
Abstract
We have identified 88 interactor candidates for human growth hormone (GH) by the yeast two-hybrid assay. Among those, we focused our efforts on carboxypeptidase E (CPE), which has been thought to play a key role in sorting prohormones, such as pro-opiomelanocortin (POMC), to regulated secretory vesicles. We found that CPE colocalizes with and interacts with GH in AtT20 pituitary cells. Downregulation of CPE led to decreased levels of GH secretion, consistent with involvement of CPE in GH sorting/secretion. Our binding assay in vitro with bacterially expressed proteins suggested that GH directly interacts with CPE but in a manner different from POMC.
Keywords
AtT20; carboxypeptidase E; growth hormone; pituitary; secretion;
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