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http://dx.doi.org/10.14348/molcells.2015.2238

Propyl Gallate Inhibits Adipogenesis by Stimulating Extracellular Signal-Related Kinases in Human Adipose Tissue-Derived Mesenchymal Stem Cells  

Lee, Jeung-Eun (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology)
Kim, Jung-Min (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology)
Jang, Hyun-Jun (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology)
Lim, Se-Young (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology)
Choi, Seon-Jeong (Department of Food Science and Technology, Korea National University of Transportation)
Lee, Nan-Hee (Department of Food Nutrition and Cook, Daegu Science University)
Suh, Pann-Ghill (School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology)
Choi, Ung-Kyu (Department of Food Science and Technology, Korea National University of Transportation)
Publication Information
Molecules and Cells / v.38, no.4, 2015 , pp. 336-342 More about this Journal
Abstract
Propyl gallate (PG) used as an additive in various foods has antioxidant and anti-inflammatory effects. Although the functional roles of PG in various cell types are well characterized, it is unknown whether PG has effect on stem cell differentiation. In this study, we demonstrated that PG could inhibit adipogenic differentiation in human adipose tissue-derived mesenchymal stem cells (hAMSCs) by decreasing the accumulation of intracellular lipid droplets. In addition, PG significantly reduced the expression of adipocyte-specific markers including peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$), CCAAT enhancer binding protein-${\alpha}$ (C/EBP-${\alpha}$), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein 2 (aP2). PG inhibited adipogenesis in hAMSCs through extracellular regulated kinase (ERK) pathway. Decreased adipogenesis following PG treatment was recovered in response to ERK blocking. Taken together, these results suggest a novel effect of PG on adipocyte differentiation in hAMSCs, supporting a negative role of ERK1/2 pathway in adipogenic differentiation.
Keywords
adipogenesis; human adipose tissue-derived mesenchymal stem cells; propyl gallate;
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1 Abdo, K.M., Huff, J.E., Haseman, J.K., and Alden, C.J. (1986). No evidence of carcinogenicity of D-mannitol and propyl gallate in F344 rats or B6C3F1 mice. Food Chem. Toxicol. 24, 1091-1097.   DOI   ScienceOn
2 Bianco, P., Robey, P.G., and Simmons, P.J. (2008). Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2, 313-319.   DOI   ScienceOn
3 Bin, H.S., and Choi, U.K. (2012). Myricetin inhibits adipogenesis in human adipose tissue-derived mesenchymal stem cells. Food Sci. Biotechnol. 21, 1391-1396.   DOI   ScienceOn
4 Camp, H.S., and Tafuri, S.R. (1997). Regulation of peroxisome proliferator-activated receptor gamma activity by mitogen-activated protein kinase. J. Biol. Chem. 272, 10811-10816.   DOI   ScienceOn
5 Chen, F.H., Rousche, K.T., and Tuan, R.S. (2006). Technology Insight: adult stem cells in cartilage regeneration and tissue engineering. Nat. Clin. Pract. Rheumatol. 2, 373-382.   DOI   ScienceOn
6 Chen, C.H., Liu, T.Z., Chen, C.H., Wong, C.H., Chen, C.H., Lu, F.J., and Chen, S.C. (2007). The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells. Mol. Nutr. Food Res. 51, 962-968.   DOI   ScienceOn
7 Crews, C.M., Alessandrini, A., and Erikson, R.L. (1992). The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258, 478-480.   DOI
8 Dacre, J.C. (1974). Long-term toxicity study of n-propyl gallate in mice. Food Cosmet. Toxicol. 12, 125-129.   DOI   ScienceOn
9 Daniel, J.W. (1986). Metabolic aspects of antioxidants and preservatives. Xenobiotica 16, 1073-1078.   DOI
10 Dezawa, M., Kanno, H., Hoshino, M., Cho, H., Matsumoto, N., Itokazu, Y., Tajima, N., Yamada, H., Sawada, H., Ishikawa, H., et al. (2004). Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation. J. Clin. Invest. 113, 1701-1710.   DOI
11 Dezawa, M., Ishikawa, H., Itokazu, Y., Yoshihara, T., Hoshino, M., Takeda, S., Ide, C., and Nabeshima, Y. (2005). Bone marrow stromal cells generate muscle cells and repair muscle degeneration. Science 309, 314-317.   DOI   ScienceOn
12 Foti, C., Bonamonte, D., Cassano, N., Conserva, A., and Vena, G.A. (2010). Allergic contact dermatitis to propyl gallate and pentylene glycol in an emollient cream. Australas. J. Dermatol. 51, 147-148.   DOI   ScienceOn
13 Hotamisligil, G.S., Shargill, N.S., and Spiegelman, B.M. (1993). Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259, 87-91.   DOI   ScienceOn
14 Gong, Z., Huang, C., Sheng, X., Zhang, Y., Li, Q., Wang, M.W., Peng, L., and Zang, Y.Q. (2009). The role of tanshinone IIA in the treatment of obesity through peroxisome proliferator-activated receptor gamma antagonism. Endocrinology 150, 104-113.   DOI
15 Han, Y.H., Moon, H.J., You, B.R., Kim, S.Z., Kim, S.H., and Park, W.H. (2010). Propyl gallate inhibits the growth of HeLa cells via caspase-dependent apoptosis as well as a G1 phase arrest of the cell cycle. Oncol. Rep. 23, 1153-1158.
16 Hirose, M., Yada, H., Hakoi, K., Takahashi, S., and Ito, N. (1993). Modification of carcinogenesis by alpha-tocopherol, t-butylhydroquinone, propyl gallate and butylated hydroxytoluene in a rat multi-organ carcinogenesis model. Carcinogenesis 14, 2359-2364.   DOI   ScienceOn
17 Hu, E., Kim, J.B., Sarraf, P., and Spiegelman, B.M. (1996). Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARgamma. Science 274, 2100-2103.   DOI   ScienceOn
18 Jeon, W.K., and Kim, B.C. (2007). WITHDRAWN: Heme oxygenase-1 mediates the anti-inflammatory effect of propyl gallate in LPS-stimulated macrophages. Biochem. Biophys. Res. Commun. 361, 645-650.   DOI   ScienceOn
19 Karthikeyan, K., Sarala Bai, B.R., Gauthaman, K., and Niranjali Devaraj, S. (2005). Protective effect of propyl gallate against myocardial oxidative stress-induced injury in rat. J. Pharm. Pharmacol. 57, 67-73.   DOI   ScienceOn
20 Kim, J., Lee, I., Seo, J., Jung, M., Kim, Y., Yim, N., and Bae, K. (2010a). Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3-L1 cells. Phytother. Res. 24, 1543-1548.   DOI   ScienceOn
21 Kim, M.H., Park, J.S., Seo, M.S., Jung, J.W., Lee, Y.S., and Kang, K.S. (2010b). Genistein and daidzein repress adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells via Wnt/beta-catenin signalling or lipolysis. Cell Prolif. 43, 594-605.   DOI   ScienceOn
22 Lee, R.H., Kim, B., Choi, I., Kim, H., Choi, H.S., Suh, K., Bae, Y.C., and Jung, J.S. (2004). Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell. Physiol. Biochem. 14, 311-324.   DOI   ScienceOn
23 Li, X., Cui, Q., Kao, C., Wang, G.J., and Balian, G. (2003). Lovastatin inhibits adipogenic and stimulates osteogenic differentiation by suppressing PPARgamma2 and increasing Cbfa1/Run$\times$2 expression in bone marrow mesenchymal cell cultures. Bone 33, 652-659.   DOI   ScienceOn
24 Lim, S., Jang, H.J., Kim, J.K., Kim, J.M., Park, E.H., Yang, J.H., Kim, Y.H., Yea, K., Ryu, S.H., and Suh, P.G. (2011). Ochratoxin A inhibits adipogenesis through the extracellular signal-related kinases-peroxisome proliferator-activated receptor-gamma pathway in human adipose tissue-derived mesenchymal stem cells. Stem Cells Dev. 20, 415-426.   DOI   ScienceOn
25 Lim, S., Jang, H.J., Park, E.H., Kim, J.K., Kim, J.M., Kim, E.K., Yea, K., Kim, Y.H., Lee-Kwon, W., Ryu, S.H., et al. (2012). Wedelolactone inhibits adipogenesis through the ERK pathway in human adipose tissue-derived mesenchymal stem cells. J. Cell. Biochem. 113, 3436-3445.   DOI   ScienceOn
26 Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S., and Marshak, D.R. (1999). Multilineage potential of adult human mesenchymal stem cells. Science 284, 143-147.   DOI   ScienceOn
27 Mimeault, M., and Batra, S.K. (2008). Recent progress on normal and malignant pancreatic stem/progenitor cell research: therapeutic implications for the treatment of type 1 or 2 diabetes mellitus and aggressive pancreatic cancer. Gut 57, 1456-1468.   DOI   ScienceOn
28 Pan, R.L., Chen, Y., Xiang, L.X., Shao, J.Z., Dong, X.J., and Zhang, G.R. (2008). Fetal liver-conditioned medium induces hepatic specification from mouse bone marrow mesenchymal stromal cells: a novel strategy for hepatic transdifferentiation. Cytotherapy 10, 668-675.   DOI   ScienceOn
29 Park, J.E., Kim, H.T., Lee, S., Lee, Y.S., Choi, U.K., Kang, J.H., Choi, S.Y., Kang, T.C., Choi, M.S., and Kwon, O.S. (2011). Differential expression of intermediate filaments in the process of developing hepatic steatosis. Proteomics 11, 2777-2789.   DOI   ScienceOn
30 Raghavan, S., and Hultin, H.O. (2005). Model system for testing the efficacy of antioxidants in muscle foods. J. Agric. Food Chem. 53, 4572-4577.   DOI   ScienceOn
31 Reddan, J.R., Giblin, F.J., Sevilla, M., Padgaonkar, V., Dziedzic, D.C., Leverenz, V.R., Misra, I.C., Chang, J.S., and Pena, J.T. (2003). Propyl gallate is a superoxide dismutase mimic and protects cultured lens epithelial cells from H2O2 insult. Exp. Eye Res. 76, 49-59.   DOI   ScienceOn
32 Rosen, E.D., Walkey, C.J., Puigserver, P., and Spiegelman, B.M. (2000). Transcriptional regulation of adipogenesis. Genes Dev. 14, 1293-1307.
33 Sermeus, A., Cosse, J.P., Crespin, M., Mainfroid, V., de Longueville, F., Ninane, N., Raes, M., Remacle, J., and Michiels, C. (2008). Hypoxia induces protection against etoposide-induced apoptosis: molecular profiling of changes in gene expression and transcription factor activity. Mol. Cancer 7, 27.
34 Rosin, M.P., and Stich, H.F. (1980). Enhancing and inhibiting effects of propyl gallate on carcinogen-induced mutagenesis. J. Environ. Pathol. Toxicol. 4, 159-167.
35 Saito, T., Abe, D., and Sekiya, K. (2009). Flavanone exhibits PPARgamma ligand activity and enhances differentiation of 3T3-L1 adipocytes. Biochem. Biophys. Res. Commun. 380, 281-285.   DOI   ScienceOn
36 Seo, J.B., Moon, H.M., Kim, W.S., Lee, Y.S., Jeong, H.W., Yoo, E.J., Ham, J., Kang, H., Park, M.G., Steffensen, K.R., et al. (2004). Activated liver X receptors stimulate adipocyte differentiation through induction of peroxisome proliferator-activated receptor gamma expression. Mol. Cell. Biol. 24, 3430-3444.   DOI
37 Shang, W., Yang, Y., Jiang, B., Jin, H., Zhou, L., Liu, S., and Chen, M. (2007). Ginsenoside Rb1 promotes adipogenesis in 3T3-L1 cells by enhancing PPARgamma2 and C/EBPalpha gene expression. Life Sci. 80, 618-625.   DOI   ScienceOn
38 Spiegelman, B.M. (1998). PPAR-gamma: adipogenic regulator and thiazolidinedione receptor. Diabetes 47, 507-514.   DOI   ScienceOn
39 Spiegelman, B.M., and Flier, J.S. (2001). Obesity and the regulation of energy balance. Cell 104, 531-543.   DOI   ScienceOn
40 Tominaga, S., Yamaguchi, T., Takahashi, S., Hirose, F., and Osumi, T. (2005). Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase. Biochem. Biophys. Commun. 326, 499-504.   DOI   ScienceOn
41 Wu, T.W., Fung, K.P., Zeng, L.H., Wu, J., and Nakamura, H. (1994). Propyl gallate as a hepatoprotector in vitro and in vivo. Biochem. Pharmacol. 48, 419-422.   DOI   ScienceOn