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http://dx.doi.org/10.14348/molcells.2015.2170

A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells  

Chen, Qian (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Zhang, Zhijian (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Liu, Jinbo (Department of Orthopedics, the Third Affiliated Hospital of Suzhou University)
He, Qinghua (School of Pharmacology, Jiangsu University)
Zhou, Yuepeng (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Shao, Genbao (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Sun, Xianglan (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Cao, Xudong (Department of Chemical Engineering, University of Ottawa)
Gong, Aihua (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Jiang, Ping (Department of Histology and Embryology, School of Medicine, Jiangsu University)
Publication Information
Molecules and Cells / v.38, no.3, 2015 , pp. 221-228 More about this Journal
Abstract
Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, $p75^{NTR}$, S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin not only promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.
Keywords
fibrin; ectomesenchymal stem cells; myelinating phenotype; schwann-like cells;
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1 Armati, P.J., and Mathey, E.K. (2014). Clinical implications of Schwann cell biology. J. Peripher. Nerv. Syst. 19, 14-23.   DOI   ScienceOn
2 Berrocal, Y.A., Almeida, V.W., Gupta, R., and Levi, A.D. (2013). Transplantation of Schwann cells in a collagen tube for the repair of large, segmental peripheral nerve defects in rats. J. Neurosurg. 119, 720-732.   DOI   ScienceOn
3 Betters, E., Liu, Y., Kjaeldgaard, A., Sundstrom, E., and Garcia-Castro, M.I. (2010). Analysis of early human neural crest development. Dev. Biol. 344, 578-592.   DOI   ScienceOn
4 Chen, P., Cescon, M., and Bonaldo, P. (2014). The role of collagens in peripheral nerve myelination and function. Mol. Neurobiol. 2014, 1-10.
5 Christopherson, G.T., Song, H., and Mao, H.Q. (2009). The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. Biomaterials 30, 556-564.   DOI   ScienceOn
6 Corbeil, D., Roper, K., Hellwig, A., Tavian, M., Miraglia, S., Watt, S.M., Simmons, P.J., Peault, B., Buck, D.W., and Huttner, W.B. (2000). The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions. J. Biol. Chem. 275, 5512-5520.   DOI   ScienceOn
7 De Santis, G., Lennon, A.B., Boschetti, F., Verhegghe, B., Verdonck, P., and Prendergast, P.J. (2011). How can cells sense the elasticity of a substrate? An analysis using a cell tensegrity model. Eur. Cell. Mater. 22, 202-213.   DOI
8 Enomoto, M., Bunge, M.B., and Tsoulfas, P. (2013). A multifunctional neurotrophin with reduced affinity to p75 enhances transplanted Schwann cell survival and axon growth after spinal cord injury. Exp. Neurol. 248C, 170-182.
9 Fowlkes, V., Wilson, C.G., Carver, W., and Goldsmith, E.C. (2013). Mechanical loading promotes mast cell degranulation via RGDintegrin dependent pathways. J. Biomech. 46, 788-795.   DOI   ScienceOn
10 Gasparotto, V.P., Landim-Alvarenga, F.C., Oliveira, A.L., Simoes, G.F., Lima-Neto, J.F., Barraviera, B., and Ferreira, R.S., Jr. (2014). A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells. Stem Cell Res. Ther. 5, 78   DOI   ScienceOn
11 Hadjipanayi, E., Mudera, V., and Brown, R.A. (2009). Close dependence of fibroblast proliferation on collagen scaffold matrix stiffness. J. Tissue Eng. Regen. Med. 3, 77-84.   DOI   ScienceOn
12 Hall, B.K. (2008). The neural crest and neural crest cells: discovery and significance for theories of embryonic organization. J. Biosci. 33, 781-793.   DOI
13 Hauser, S., Widera, D., Qunneis, F., Muller, J., Zander, C., Greiner, J., Strauss, C., Luningschror, P., Heimann, P., Schwarze, H., et al. (2012). Isolation of novel multipotent neural crest-derived stem cells from adult human inferior turbinate. Stem Cells Dev. 21, 742-756.   DOI   ScienceOn
14 Jacob, C., Lotscher, P., Engler, S., Baggiolini, A., Varum Tavares, S., Brugger, V., John, N., Buchmann-Moller, S., Snider, P.L., Conway, S.J., et al. (2014). HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia. J. Neurosci. 34, 6112-6122.   DOI   ScienceOn
15 Linnes, M.P., Ratner, B.D., and Giachelli, C.M. (2007). A fibrinogenbased precision microporous scaffold for tissue engineering. Biomaterials 28, 5298-5306.   DOI   ScienceOn
16 Kang, B.J., Kim, H., Lee, S.K., Kim, J., Shen, Y., Jung, S., Kang, K.S., Im, S.G., Lee, S.Y., Choi, M., et al. (2014). Umbilical-cordblood-derived mesenchymal stem cells seeded onto fibronectinimmobilized polycaprolactone nanofiber improve cardiac function. Acta Biomater. 10, 3007-3017   DOI   ScienceOn
17 Kondo, Y., Wenger, D.A., Gallo, V., and Duncan, I.D. (2005). Galactocerebrosidase-deficient oligodendrocytes maintain stable central myelin by exogenous replacement of the missing enzyme in mice. Proc. Natl. Acad. Sci. USA 102, 18670-18675.   DOI   ScienceOn
18 Lin, Y., Yan, Z., Liu, L., Qiao, J., Jing, W., Wu, L., Chen, X., Li, Z., Tang, W., Zheng, X., et al. (2006). Proliferation and pluripotency potential of ectomesenchymal cells derived from first branchial arch. Cell Prolif. 39, 79-92.   DOI   ScienceOn
19 Linsley, C., Wu, B., and Tawil, B. (2013). The effect of fibrinogen, collagen type I, and fibronectin on mesenchymal stem cell growth and differentiation into osteoblasts. Tissue Eng. Part A 19, 1416-1423.   DOI
20 Liu, J., Chen, Q., Zhang, Z., Zheng, Y., Sun, X., Cao, X., Gong, A., Cui, Y., He, Q., and Jiang, P. (2013). Fibrin scaffolds containing ectomesenchymal stem cells enhance behavioral and histological improvement in a rat model of spinal cord injury. Cells Tissues Organs 198, 35-46.   DOI   ScienceOn
21 Lu, P., Wang, Y., Graham, L., McHale, K., Gao, M., Wu, D., Brock, J., Blesch, A., Rosenzweig, E.S., Havton, L.A., et al. (2012). Long-distance growth and connectivity of neural stem cells after severe spinal cord injury. Cell 150, 1264-1273.   DOI   ScienceOn
22 Natale, C.F., Ventre, M., and Netti, P.A. (2014). Tuning the materialcytoskeleton crosstalk via nanoconfinement of focal adhesions. Biomaterials 35, 2743-2751.   DOI   ScienceOn
23 Lu, D., Luo, C., Zhang, C., Li, Z., and Long, M. (2014). Differential regulation of morphology and stemness of mouse embryonic stem cells by substrate stiffness and topography. Biomaterials 35, 3945-3955.   DOI   ScienceOn
24 Lutz, D., Loers, G., Kleene, R., Oezen, I., Kataria, H., Katagihallimath, N., Braren, I., Harauz, G., and Schachner, M. (2014). Myelin basic protein cleaves cell adhesion molecule L1 and promotes neuritogenesis and cell survival. J. Biol. Chem. 289, 13503-13518   DOI   ScienceOn
25 McKee, K.K., Yang, D.H., Patel, R., Chen, Z.L., Strickland, S., Takagi, J., Sekiguchi, K., and Yurchenco, P.D. (2012). Schwann cell myelination requires integration of laminin activities. J. Cell Sci. 125, 4609-4619   DOI
26 Ness, J.K., Snyder, K.M., and Tapinos, N. (2013). Lck tyrosine kinase mediates beta1-integrin signalling to regulate Schwann cell migration and myelination. Nat. Commun. 4, 1912.   DOI   ScienceOn
27 Nie, X., Xing, Y., Deng, M., Gang, L., Liu, R., Zhang, Y., and Wen, X. (2014). Ecto-mesenchymal stem cells from facial process: potential for muscle regeneration. Cell Biochem. Biophys. 70, 615-622.   DOI   ScienceOn
28 Oh, S., Brammer, K.S., Li, Y.S., Teng, D., Engler, A.J., Chien, S., and Jin, S. (2009). Stem cell fate dictated solely by altered nanotube dimension. Proc. Natl. Acad. Sci. USA 106, 2130-2135.   DOI   ScienceOn
29 Radtke, C., Sasaki, M., Lankford, K.L., Gallo, V., and Kocsis, J.D. (2011). CNPase expression in olfactory ensheathing cells. J. Biomed. Biotechnol. 2011, 608496.
30 Richardson, G.D., Robson, C.N., Lang, S.H., Neal, D.E., Maitland, N.J., and Collins, A.T. (2004). CD133, a novel marker for human prostatic epithelial stem cells. J. Cell Sci. 117, 3539-3545.   DOI   ScienceOn
31 Riopel, M., Stuart, W., and Wang, R. (2013). Fibrin improves beta (INS-1) cell function, proliferation and survival through integrin v3. Acta Biomater. 9, 8140-8148   DOI   ScienceOn
32 Rutten, M.J., Janes, M.A., Chang, I.R., Gregory, C.R., and Gregory, K.W. (2012). Development of a functional schwann cell phenotype from autologous porcine bone marrow mononuclear cells for nerve repair. Stem Cells Int. 2012, 738484.
33 Schurmann, M., Wolff, A., Widera, D., Hauser, S., Heimann, P., Hutten, A., Kaltschmidt, C., and Kaltschmidt, B. (2014). Interaction of adult human neural crest-derived stem cells with a nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Res. 13, 98-110.   DOI   ScienceOn
34 Sharp, K.G., Yee, K.M., and Steward, O. (2014). A re-assessment of long distance growth and connectivity of neural stem cells after severe spinal cord injury. Exp. Neurol. 257, 186-204.   DOI   ScienceOn
35 Sullivan, A.M., and Toulouse, A. (2011). Neurotrophic factors for the treatment of Parkinson's disease. Cytokine Growth Factor Rev. 22, 157-165.   DOI   ScienceOn
36 Tian, X., Wang, S., Zhang, Z., and Lv, D. (2012). Rat bone marrowderived Schwann-like cells differentiated by the optimal inducers combination on microfluidic chip and their functional performance. PLoS One 7, e42804.   DOI
37 Wang, X., and Xu, X.M. (2014). Long-term survival, axonal growthpromotion, and myelination of Schwann cells grafted into contused spinal cord in adult rats. Exp. Neurol. 261, 308-319   DOI   ScienceOn
38 Yan, Z., Lin, Y., Jiao, X., Li, Z., Wu, L., Jing, W., Qiao, J., Liu, L., Tang, W., Zheng, X., et al. (2006). Characterization of ectomesenchymal cells isolated from the first branchial arch during multilineage differentiation. Cells Tissues Organs 183, 123-132.   DOI   ScienceOn
39 Wei, Y., Gong, K., Zheng, Z., Liu, L., Wang, A., Zhang, L., Ao, Q., Gong, Y., and Zhang, X. (2010). Schwann-like cell differentiation of rat adipose-derived stem cells by indirect co-culture with Schwann cells in vitro. Cell Prolif. 43, 606-616.   DOI   ScienceOn
40 Weis, S., Lee, T.T., Del Campo, A., and Garcia, A.J. (2013). Dynamic cell-adhesive microenvironments and their effect on myogenic differentiation. Acta. Biomater. 9, 8059-8066.   DOI   ScienceOn
41 Yazdani, S.O., Hafizi, M., Zali, A.R., Atashi, A., Ashrafi, F., Seddighi, A.S., and Soleimani, M. (2013). Safety and possible outcome assessment of autologous Schwann cell and bone marrow mesenchymal stromal cell co-transplantation for treatment of patients with chronic spinal cord injury. Cytotherapy 15, 782-791.   DOI   ScienceOn
42 Zhang, J., Shi, Q., Yang, P., Xu, X., Chen, X., Qi, C., Zhang, J., Lu, H., Zhao, B., Zheng, P., et al. (2012). Neuroprotection of neurotrophin-3 against focal cerebral ischemia/reperfusion injury is regulated by hypoxia-responsive element in rats. Neuroscience 222, 1-9   DOI   ScienceOn
43 Zhu, T., Tang, Q., Gao, H., Shen, Y., Chen, L., and Zhu, J. (2014). Current status of cell-mediated regenerative therapies for human spinal cord injury. Neurosci. Bull. 30, 671-682.   DOI   ScienceOn