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http://dx.doi.org/10.14348/molcells.2015.2255

A Cytosolic Thioredoxin Acts as a Molecular Chaperone for Peroxisome Matrix Proteins as Well as Antioxidant in Peroxisome  

Du, Hui (Department of Biological Science, Sookmyung Women's University)
Kim, Sunghan (Department of Plant Science, Seoul National University)
Hur, Yoon-Sun (Department of Biological Science, Sookmyung Women's University)
Lee, Myung-Sok (Department of Biological Science, Sookmyung Women's University)
Lee, Suk-Ha (Department of Plant Science, Seoul National University)
Cheon, Choong-Ill (Department of Biological Science, Sookmyung Women's University)
Publication Information
Molecules and Cells / v.38, no.2, 2015 , pp. 187-194 More about this Journal
Abstract
Thioredoxin (TRX) is a disulfide reductase present ubiquitously in all taxa and plays an important role as a regulator of cellular redox state. Recently, a redox-independent, chaperone function has also been reported for some thioredoxins. We previously identified nodulin-35, the subunit of soybean uricase, as an interacting target of a cytosolic soybean thioredoxin, GmTRX. Here we report the further characterization of the interaction, which turns out to be independent of the disulfide reductase function and results in the co-localization of GmTRX and nodulin-35 in peroxisomes, suggesting a possible function of GmTRX in peroxisomes. In addition, the chaperone function of GmTRX was demonstrated in in vitro molecular chaperone activity assays including the thermal denaturation assay and malate dehydrogenase aggregation assay. Our results demonstrate that the target of GmTRX is not only confined to the nodulin-35, but many other peroxisomal proteins, including catalase (AtCAT), transthyretin-like protein 1 (AtTTL1), and acyl-coenzyme A oxidase 4 (AtACX4), also interact with the GmTRX. Together with an increased uricase activity of nodulin-35 and reduced ROS accumulation observed in the presence of GmTRX in our results, especially under heat shock and oxidative stress conditions, it appears that GmTRX represents a novel thioredoxin that is co-localized to the peroxisomes, possibly providing functional integrity to peroxisomal proteins.
Keywords
chaperone; peroxisome; thioredoxin; uricase;
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