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http://dx.doi.org/10.14348/molcells.2015.2229

Galangin and Kaempferol Suppress Phorbol-12-Myristate-13-Acetate-Induced Matrix Metalloproteinase-9 Expression in Human Fibrosarcoma HT-1080 Cells  

Choi, Yu Jung (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
Lee, Young Hun (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
Lee, Seung-Taek (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
Publication Information
Molecules and Cells / v.38, no.2, 2015 , pp. 151-155 More about this Journal
Abstract
Matrix metalloproteinase (MMP)-9 degrades type IV collagen in the basement membrane and plays crucial roles in several pathological implications, including tumorigenesis and inflammation. In this study, we analyzed the effect of flavonols on MMP-9 expression in phorbol-12-myristate-13-acetate (PMA)-induced human fibrosarcoma HT-1080 cells. Galangin and kaempferol efficiently decreased MMP-9 secretion, whereas fisetin only weakly decreased its secretion. Galangin and kaempferol did not affect cell viability at concentrations up to $30{\mu}M$. Luciferase reporter assays showed that galangin and kaempferol decrease transcription of MMP-9 mRNA. Moreover, galangin and kaempferol strongly reduce $I{\kappa}B{\alpha}$ phosphorylation and significantly decrease JNK phosphorylation. These results indicate that galangin and kaempferol suppress PMA-induced MMP-9 expression by blocking activation of NF-${\kappa}B$ and AP-1. Therefore, these flavonols could be used as chemopreventive agents to lower the risk of diseases involving MMP-9.
Keywords
flavonol; galangin; kaempferol; mitogen-activated protein kinase; MMP-9; NF-${\kappa}B$;
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