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http://dx.doi.org/10.14348/molcells.2015.2202

A Role for Peroxidasin PXN-1 in Aspects of C. elegans Development  

Lee, Juyeon (Department of Life Science, Gwangju Institute of Science and Technology)
Bandyopadhyay, Jaya (Department of Biotechnology, West Bengal University of Technology)
Lee, Jin Il (Division of Biological Science and Technology, College of Science and Technology, Yonsei University)
Cho, Injeong (Department of Biology Education, College of Education, Chosun University)
Park, Daeho (Department of Life Science, Gwangju Institute of Science and Technology)
Cho, Jeong Hoon (Department of Biology Education, College of Education, Chosun University)
Publication Information
Molecules and Cells / v.38, no.1, 2015 , pp. 51-57 More about this Journal
Abstract
The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.
Keywords
C. elegans; heat stress; neuronal development; peroxidasins; pxn-1;
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