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http://dx.doi.org/10.1007/s10059-009-0160-6

Distinct Roles for JNK1 and JNK3 During TNF-α- or Etoposide-Induced Apoptosis in HeLa Cells  

Ham, Young-Mi (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University)
Lim, Jin-Hee (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University)
Lee, Seung-Ki (Division of Pharmaceutical Biosciences, College of Pharmacy and the Research Institute for Pharmaceutical Sciences, Seoul National University)
Publication Information
Molecules and Cells / v.28, no.6, 2009 , pp. 509-513 More about this Journal
Abstract
Here, we show that JNK1 and JNK3 have different roles in ${\alpha}-$ or etoposide-induced apoptosis in HeLa cells. Dominant negative JNK1 inhibited $TNF-{\alpha}-$ or etoposide-induced apoptosis, while dominant negative JNK3 promoted $TNF-{\alpha}-$ or etoposide-induced apoptosis. During $TNF-{\alpha}$-induced apoptosis, JNK1 was activated in a biphasic manner, exhibiting both transient and sustained activity, whereas JNK3 was activated early and in a transient manner. The role of JNK3 activation was an anti-apoptotic effect, while the role of JNK1 activation was a pro-apoptotic effect. These results suggest that the anti-apoptotic mechanism of JNK3 in $TNF-{\alpha}$-induced apoptosis originates before the apoptotic machinery is triggered.
Keywords
anti-apoptotic; apoptosis; etoposide; JNK1; JNK3; survival; TNF-${\alpha}$;
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