Browse > Article
http://dx.doi.org/10.1007/s10059-009-0107-y

Structural and Quantitative Expression Analyses of HERV Gene Family in Human Tissues  

Ahn, Kung (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Kim, Heui-Soo (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Publication Information
Molecules and Cells / v.28, no.2, 2009 , pp. 99-103 More about this Journal
Abstract
Human endogenous retroviruses (HERVs) have been implicated in the pathogenesis of several human diseases as multi-copy members in the human genome. Their gene expression profiling could provide us with important insights into the pathogenic relationship between HERVs and cancer. In this study, we have evaluated the genomic structure and quantitatively determined the expression patterns in the env gene of a variety of HERV family members located on six specific loci by the RetroTector 10 program, as well as real-time RT-PCR amplification. The env gene transcripts evidenced significant differences in the human tumor/normal adjacent tissues (colon, liver, uterus, lung and testis). As compared to the adjacent normal tissues, high levels of expression were noted in testis tumor tissues for HERV-K, in liver and lung tumor tissues for HERV-R, in liver, lung, and testis tumor tissues for HERV-H, and in colon and liver tumor tissues for HERV-P. These data warrant further studies with larger groups of patients to develop biomarkers for specific human cancers.
Keywords
biomarker; HERV env gene; real-time RT-PCR; tumor tissues;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
연도 인용수 순위
1 Antony, J.M., Zhu, Y., Izad, M., Warren, K.G., Vodjgani, M., Mallet, F., and Power, C. (2007). Comparative expression of human endogenous retrovirus-W genes in multiple sclerosis. AIDS Res. Hum. Retroviruses 23, 1251-1256   DOI   ScienceOn
2 Blikstad, V., Benachenhou, F., Sperber, G.O., and Blomberg, J. (2008). Evolution of human endogenous retroviral sequences: a conceptual account. Cell. Mol. Life Sci. 65, 3348-3365   DOI   ScienceOn
3 Goedert, J.J., Sauter, M.E., Jacobson, L.P., Vessella, R.L., Hilgartner, M.W., Leitman, S.F., Fraser, M.C., and Mueller-Lantzsch, N.G. (1999). High prevalence of antibodies against HERV-K10 in patients with testicular cancer but not with AIDS. Cancer Epidemiol. Biomarkers Prev. 8, 293-296
4 Golan, M., Hizi, A., Resau, J.H., Yaal-Hahoshen, N., Reichman, H., Keydar, I., and Tsarfaty, I. (2008). Human endogenous retrovirus (HERV-K) reverse transcriptase as a breast cancer prognostic marker. Neoplasia 10, 521-533   DOI   PUBMED
5 Lindeskog, M., Mager, D.L., and Blomberg, J. (1999). Isolation of a human endogenous retroviral HERV-H element with an open env reading frame. Virology 258, 441-450   DOI   ScienceOn
6 Nellaker, C., Yao, Y., Jones-Brando, L., Mallet, F., Yolken, R.H., and Karlsson, H. (2006). Transactivation of elements in the human endogenous retrovirus W family by viral infection. Retrovirology 3, 44   DOI   ScienceOn
7 Prusty, B.K., zur Hausen, H., Schmidt, R., Kimmel, R., and de Villiers, E.M. (2008). Transcription of HERV-E and HERV-Erelated sequences in malignant and non-malignant human haematopoietic cells. Virology 382, 37-45   DOI   ScienceOn
8 Sperber, G.O., Airola, T., Jern, P., and Blomberg, J. (2007). Automated recognition of retroviral sequences in genomic data-- RetroTector. Nucleic Acids Res. 35, 4964-4976   DOI   ScienceOn
9 Yi, J.M., Kim, H.M., and Kim, H.S. (2004b). Expression of the human endogenous retrovirus HERV-W family in various human tissues and cancer cells. J. Gen. Virol. 85, 1203-1210   DOI   ScienceOn
10 Yi, J.M., Schuebel, K., and Kim, H.S. (2006a). Molecular genetic analyses of human endogenous retroviral elements belonging to the HERV-P family in primates, human tissues, and cancer cells. Genomics 89, 1-9   DOI   ScienceOn
11 Ruprecht, K., Mayer, J., Sauter, M., Roemer, K., and Mueller- Lantzsch, N. (2008b). Endogenous retroviruses and cancer. Cell. Mol. Life. Sci. 65, 3366-3382   DOI   ScienceOn
12 Dupressoir, A., Marceau, G., Vernochet, C., Benit, L., Kanellopoulos, C., Sapin, V., and Heidmann, T. (2005). Syncytin-A and syncytin- B, two fusogenic placenta-specific murine envelope genes of retroviral origin conserved in Muridae. Proc. Natl. Acad. Sci. USA 102, 725-730   DOI   ScienceOn
13 Harris, J.R. (1998). Placental endogenous retrovirus (ERV): structural, functional, and evolutionary significance. Bioessays 20, 307-316   DOI   PUBMED   ScienceOn
14 Blond, J.L., Lavillette, D., Cheynet, V., Bouton, O., Oriol, G., Chapel- Fernandes, S., Mandrand, B., Mallet, F., and Cosset, F.L. (2000). An envelope glycoprotein of the human endogenous retrovirus HERV-W is expressed in the human placenta and fuses cells expressing the type D mammalian retrovirus receptor. J. Virol. 74, 3321-3329   DOI   ScienceOn
15 Bessis, D., Moles, J.P., Basset-Seguin, N., Tesniere, A., Arpin, C., and Guilhou, J.J. (2004). Differential expression of a human endogenous retrovirus E transmembrane envelope glycoprotein in normal, psoriatic and atopic dermatitis human skin. British J. Dermatol. 151, 737-745   DOI   ScienceOn
16 de Parseval, N., Lazar, V., Casella, J.F., Benit, L., and Heidmann, T. (2003). Survey of human genes of retroviral origin: identification and transcriptome of the genes with coding capacity for complete envelope proteins. J. Virol. 77, 10414-10422   DOI
17 Frank, O., Verbeke, C., Schwarz, N., Mayer, J., Fabarius, A., Hehlmann, R., Leib-Mosch, C., and Seifarth, W. (2008). Variable transcriptional activity of endogenous retroviruses in human breast cancer. J. Virol. 82, 1808-1818   DOI   ScienceOn
18 Yi, J.M., Schuebel, K., and Kim, H.S. (2007). Molecular genetic analyses of human endogenous retroviral elements belonging to the HERV-P family in primates, human tissues, and cancer cells. Genomics 89, 1-9   DOI   ScienceOn
19 Serafino, A., Balestrieri, E., Pierimarchi, P., Matteucci, C., Moroni, G., Oricchio, E., Rasi, G., Mastino, A., Spadafora, C., Garaci, E., et al. (2009). The activation of human endogenous retrovirus K (HERVK) is implicated in melanoma cell malignant transformation. Exp. Cell Res. 315, 849-862   DOI   ScienceOn
20 Kim, H.S., Yi, J.M., Hirai, H., Huh, J.W., Jeong, M.S., Jang, S.B., Kim, C.G., Saitou, N., Hyun, B.H., and Lee, W.H. (2006). Human Endogenous Retrovirus (HERV)-R family in primates: chromosomal location, gene expression, and evolution. Gene 370, 34-42   DOI   PUBMED   ScienceOn
21 Tristem, M. (2000). Identification and characterization of the novel human endogenous retrovirus families by phylogenetic screening of the human genome mapping project database. J. Virol. 74, 3715-3730   DOI   PUBMED   ScienceOn
22 Tanaka, S., Ikeda, H., Otsuka, N., Yamamoto, Y., Sugaya, T., and Yoshiki, T. (2003). Tissue specific high level expression of a full length human endogenous retrovirus genome transgene, HERV-R, under control of its own promoter in rats. Transgenic Res. 12, 319-328   DOI   ScienceOn
23 Yi, J.M., and Kim, H.S. (2007a). Molecular phylogenetic analysis of the human endogenous retrovirus E (HERV-E) family in human tissues and human cancers. Genes Genet. Syst. 82, 89-98   DOI   ScienceOn
24 Esnault, C., Priet, S., Ribet, D., Vernochet, C., Bruls, T., Lavialle, C., Weissenbach, J., and Heidmann, T. (2008). A placenta-specific receptor for the fusogenic, endogenous retrovirus-derived, human syncytin-2. Proc. Natl. Acad. Sci. USA 105, 17532-17537   DOI   ScienceOn
25 Mayer, J., Ehlhardt, S., Seifert, M., Sauter, M., Muller-Lantzsch, N., Mehraein, Y., Zang, K.D., and Meese, E. (2004). Human endogenous retrovirus HERV-K (HML-2) proviruses with Rec pro tein coding capacity and transcriptional activity. Virology 322, 190-198   DOI   ScienceOn
26 Yi, J.M., and Kim, H.S. (2007b). Expression and phylogenetic analyses of human endogenous retrovirus HC2 belonging to the HERV-T family in human tissues and cancer cells. J. Hum. Genet. 52, 285-296   DOI   ScienceOn
27 Blaise, S., de Parseval, N., Benit, L., and Heidmann, T. (2003). Genomewide screening for fusogenic human endogenous retrovirus envelopes identifies syncytin 2, a gene conserved on primate evolution. Proc. Natl. Acad. Sci . USA 100, 13013-13018   DOI   ScienceOn
28 Chang, C., Chen, P.T., Chang, G.D., Huang, C.J., and Chen, H. (2004). Functional characterization of the placental fusogenic membrane protein syncytin. Biol. Reprod. 71, 1956-1962   DOI   ScienceOn
29 Kim, H.S., Ahn, K., and Kim, D.S. (2008b). Quantitative expression of the HERV-W env gene in human tissues. Arch. Virol. 153, 1587-1591   DOI   ScienceOn
30 Ruprecht, K., Ferreira, H., Flockerzi, A., Wahl, S., Sauter, M., Mayer, J., and Mueller-Lantzsch, N. (2008a). Human endogenous retrovirus family HERV-K (HML-2) RNA transcripts are selectively packaged into retroviral particles produced by the human germ cell tumor line Tera-1 and originate mainly from a provirus on chromosome 22q11.21. J. Virol. 82, 10008-10016   DOI   ScienceOn
31 Wang-Johanning, F., Liu, J., Rycaj, K., Huang, M., Tsai, K., Rosen, D.G., Chen, D.T., Lu, D.W., Barnhart, K.F., and Johanning, G.L. (2007). Expression of multiple human endogenous retrovirus surface envelope proteins in ovarian cancer. Int. J. Cancer 120, 81-90   DOI   ScienceOn
32 Blaise, S., de Parseval, N., and Heidmann, T. (2005). Functional characterization of two newly identified human endogenous retrovirus coding envelope genes. Retrovirology 2, 19   DOI   ScienceOn
33 Yi, J.M., and Kim, H.S. (2004). Expression analysis of endo-genous retroviral elements belonging to the HERV-F family from human tissues and cancer cells. Cancer Lett. 211, 89-96   DOI   ScienceOn
34 Wang-Johanning, F., Radvanyi, L., Rycaj, K., Plummer, J.B., Yan, P., Sastry, K.J., Piyathilake, C.J., Hunt, K.K., and Johanning, G.L. (2008). Human endogenous retrovirus K triggers an antigenspecific immune response in breast cancer patients. Cancer Res. 68, 5869-5877   DOI   ScienceOn
35 Yi, J.M., Kim, T.H., Huh, J.W., Park, K.S., Jang, S.B., Kim, H.M., and Kim, H.S. (2004a). Human endogenous retroviral elements belonging to the HERV-S family from human tissues, cancer cells, and primates: expression, structure, phylogeny and evolution. Gene 342, 283-392   DOI   ScienceOn
36 Kim, Y.J., Huh, J.W., Kim, D.S., Bae, M.I., Lee, J.R., Ahn, K., Kim, T.O., Song, K.A., and Kim, H.S. (2009). Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker. J. Cancer Res. Clin. Oncol. 135, 265-270   DOI   ScienceOn
37 Yi, J.M., Kim, H.M., and Kim, H.S. (2006b). Human endogenous retrovirus HERV-H family in human tissues and cancer cells: expression, identification, and phylogeny. Cancer Lett. 231, 228-239   DOI   ScienceOn
38 Kim, T.H., Jeon, Y.J., Yi, J.M., Kim, D.S., Huh, J.W., Hur, C.G., and Kim, H.S. (2004). The distribution and expression of HERV families in the human genome. Mol. Cells 18, 87-93
39 Kim, H.S., Kim, D.S., Huh, J.W., Ahn, K., Yi, J.M., Lee, J.R., and Hirai, H. (2008a). Molecular characterization of the HERV-W env gene in humans and primates: expression, FISH, phylogeny, and evolution. Mol. Cells 26, 53-60   PUBMED