Browse > Article
http://dx.doi.org/10.1007/s10059-009-0097-9

Centrobin/Nip2 Expression In Vivo Suggests Its Involvement in Cell Proliferation  

Lee, Jungmin (Department of Biological Sciences, Seoul National University)
Kim, Sunmi (Department of Biological Sciences, Seoul National University)
Jeong, Yeontae (Department of Biological Sciences, Seoul National University)
Rhee, Kunsoo (Department of Biological Sciences, Seoul National University)
Abstract
Centrobin/Nip2 was initially identified as a centrosome protein that is critical for centrosome duplication and spindle assembly. In the present study, we determined the expression and subcellular localization of centrobin in selected mouse tissues. Immunoblot analysis revealed that the centrobin-specific band of 100 kDa was detected in all tissues tested but most abundantly in the thymus, spleen and testis. In the testis, centrobin was localized at the centrosomes of spermatocytes and early round spermatids, but no specific signal was detected in late round spermatids and elongated spermatids. Our results also revealed that the centrosome duplication occurs at interphase of the second meiotic division of the mouse male germ cells. The centrobin protein was more abundant in the mitotically active ovarian follicular cells and thymic cortex cells than in non-proliferating corpus luteal cells and thymic medullary cells. The expression pattern of centrobin suggests that the biological functions of centrobin are related to cell proliferation. Consistent with the proposal, we observed reduction of the centrobin levels when NIH3T3 became quiescent in the serum-starved culture conditions. However, a residual amount of centrobin was also detected at the centrosomes of the resting cells, suggesting its role for maintaining integrity of the centrosome, especially of the daughter centriole in the cells.
Keywords
cell cycle; centrobin; centrosome; meiosis; NIP2; spermatogenesis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
연도 인용수 순위
1 Loncarek, J., and Khodjakov, A. (2009). Ab ovo or novo? Mechanisms of centriole duplication. Mol. Cells 27, 135-142   DOI   ScienceOn
2 Manandhar, G., Schatten, H., and Sutovsky, P. (2005). Centrosome reduction during gametogenesis and its significance. Biol. Reprod. 72, 2-13   DOI   ScienceOn
3 Yamashita, S., and Okada, Y. (2005). Application of heat-induced antigen retrieval to aldehyde-fixed fresh frozen sections. J. Histochem. Cytochem. 53, 1421-1432
4 Bettencourt-Dias, M., and Carvalho-Santos, Z. (2008). Double life of centrioles: CP110 in the spotlight. Trends Cell Biol. 18, 8-11   DOI   ScienceOn
5 Jeong, Y., Lee, J., Kim, K., Yoo, J.C., and Rhee, K. (2007). Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization. J. Cell Sci. 120, 2106-2016   DOI   ScienceOn
6 Piel, M., Meyer, P., Khodjakov, A., Rieder, C.L., and Bornens, M. (2000). The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells. J. Cell Biol. 149, 317-330   DOI   ScienceOn
7 Tsou, M.F., and Stearns, T. (2006). Mechanism limiting centrosome duplication to once per cell cycle. Nature 442, 947-951   DOI   ScienceOn
8 Zou, C., Li, J., Bai, Y., Gunning, W.T., Wazer, D.E., Band, V., and Gao, Q. (2005). Centrobin: a novel daughter centriole-associated protein that is required for centriole duplication. J. Cell Biol. 171, 437-445   DOI   ScienceOn
9 Jackman, M., Lindon, C., Nigg, E.A., and Pines, J. (2003). Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat. Cell Biol. 5, 143-148   DOI   ScienceOn
10 Kramer, A., Mailand, N., Lukas, C., Syljuasen, R.G., Wilkinson, C.J., Nigg, E.A., Bartek, J., and Lukas, J. (2004). Centrosomeassociated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase. Nat. Cell Biol. 6, 884-891   DOI   ScienceOn
11 Andersen, J.S., Wilkinson, C.J., Mayor, T., Mortensen, P., Nigg, E.A., and Mann, M. (2003). Proteomic characterization of the human centrosome by protein correlation profiling. Nature 426, 570-574   DOI   ScienceOn
12 Bornens, M. (2002). Centrosome composition and microtubule anchoring mechanisms. Curr. Opin. Cell Biol. 14, 25-34   DOI   PUBMED   ScienceOn
13 Saunders, P.T., Turner, J.M., Ruggiu, M., Taggart, M., Burgoyne, P.S., Elliott, D., and Cooke, H.J. (2003). Absence of mDazl produces a final block on germ cell development at meiosis. Reproduction 126, 589-597   DOI   PUBMED
14 Blagden, S.P., and Glover, D.M. (2003). Polar expeditions--provisioning the centrosome for mitosis. Nat. Cell Biol. 5, 505-511   DOI   ScienceOn
15 Krioutchkova, M.M., and Onishchenko, G.E. (1999). Structural and functional characteristics of the centrosome in gametogenesis and early embryogenesis of animals. Int. Rev. Cytol. 185, 107-156   DOI   PUBMED
16 Nigg, E.A. (2007). Centrosome duplication: of rules and licenses. Trends Cell Biol. 17, 215-221   DOI   PUBMED   ScienceOn
17 Rhee, K., and Wolgemuth, D.J. (1997). The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes. Development 124, 2167-2177   PUBMED