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http://dx.doi.org/10.1007/s10059-009-0081-4

The Calmodulin-Binding Transcription Factor OsCBT Suppresses Defense Responses to Pathogens in Rice  

Koo, Sung Cheol (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Choi, Man Soo (National Institute of Crop Science, Rural Development Administration)
Chun, Hyun Jin (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Shin, Dong Bum (National Institute of Crop Science, Rural Development Administration)
Park, Bong Soo (Department of Plant Science, Seoul National University)
Kim, Yul Ho (National Institute of Crop Science, Rural Development Administration)
Park, Hyang-Mi (National Institute of Crop Science, Rural Development Administration)
Seo, Hak Soo (Department of Plant Science, Seoul National University)
Song, Jong Tae (School of Applied Biosciences, Kyungpook National University)
Kang, Kyu Young (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Yun, Dae-Jin (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Chung, Woo Sik (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Cho, Moo Je (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Kim, Min Chul (Division of Applied Life Science (Brain Korea 21 program), Plant Molecular Biology and Biotechnology Research Center and Environmental Biotechnology National Core Research Center, Gyeongsang National University)
Publication Information
Molecules and Cells / v.27, no.5, 2009 , pp. 563-570 More about this Journal
Abstract
We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.
Keywords
bacterial blight pathogen; CaM-binding protein; plant defense signaling; rice blast fungus; transcription factor;
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