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http://dx.doi.org/10.1007/s10059-009-0070-7

Monophyly of the Family Desmoscolecidae (Nematoda, Demoscolecida) and Its Phylogenetic Position Inferred from 18S rDNA Sequences  

Hwang, Ui Wook (Department of Biology, Teachers College and Department of Biology, Graduate School, Kyungpook National University)
Choi, Eun Hwa (Department of Biology, Teachers College and Department of Biology, Graduate School, Kyungpook National University)
Kim, Dong Sung (Marine Resources Research Department, Korea Ocean Research and Development Institute)
Decraemer, Wilfrida (Department of Invertebrates, Royal Belgian Institute of Natural Sciences)
Chang, Cheon Young (Department of Biological Science, College of Natural Sciences, Daegu University)
Publication Information
Molecules and Cells / v.27, no.5, 2009 , pp. 515-523 More about this Journal
Abstract
To infer the monophyletic origin and phylogenetic relationships of the order Desmoscolecida, a unique and puzzling group of mainly free-living marine nematodes, we newly determined nearly complete 18S rDNA sequences for six marine desmoscolecid nematodes belonging to four genera (Desmoscolex, Greeffiella, Tricoma and Paratricoma). Based on the present data and those of 72 nematode species previously reported, the first molecular phylogenetic analysis focusing on Desmoscolecida was done by using neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI) methods. All four resultant trees consistently and strongly supported that the family Desmoscolecidae forms a monophyletic group with very high node confidence values. The monophyletic clade of desmocolecid nematodes was placed as a sister group of the clade including some members of Monhysterida and Araeolaimida, Cyartonema elegans (Cyartonematidae) and Terschellingia Iongicaudata (Linhomoeidae) in all the analyses. However, the present phylogenetic trees do not show any direct attraction between the families Desmoscolecidae and Cyartonematidae. Within the monophyletic clade of the family Desmoscolecidae in all of the present phylogenetic trees, there were consistently observed two distinct subgroups which correspond to the subfamilies Desmoscolecinae [Greeffiella sp. + Desmoscolex sp.] and Tricominae [Paratricoma sp. + Tricoma sp].
Keywords
18S rDNA; Desmoscolecidae; molecular phylogeny; Nematoda;
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1 Blaxter, M.L., De Ley, P., Garey, J.R., Liu, L.X., Scheldemann, P., Vierstraete, A., Vanfleteren, J.R., Mackey, L.Y., Dorris, M., Frisse, L.M., et al. (1998). A molecular evolutionary framework for the phylum Nematoda. Nature 392, 71-75   DOI   ScienceOn
2 Chitwood, B.G., and Chitwood, M.B. (1950). An Introduction to Nema-Tology, 2nd ed. (Baltimore: Monumental Printing Company)
3 De Ley, P., Decraemer, W., and Abebe E. (2006). Introduction: summary of present knowledge and research addressing the ecology and taxonomy of freshwater nematodes. In Freshwater Nematodes, E. Abebe, I. Andrassy, and W. Traunspurger, eds. (CABI Publishing), pp. 3-30
4 Maggenti, A.R. (1983). Nematode higher classification as in-fluenced by species and family concepts. In Concepts in Nematode Systematics. The systematics association special volume no. 22, A.R. Stone, H.M. Platt, and L.F. Khalil, eds. (London, UK: Academic Press), pp. 25-40
5 Rambaut, A. (1996). Se-Al: Sequence Alignment Editor. Available at http://evolve.zoo.ox.ac.uk/
6 Rho, H.S., Kim, W., and Chang, C.Y. (2007). Description of two new free-living marine nematode species of the subgenus Desmolorenzenia (Desmoscolecida, genus Desmoscolex) from Korea. J.Nat. His. 41, 313-326   DOI   ScienceOn
7 Decraemer, W. (1985). Revision and phylogenetic systematics of the Desmoscolecida (Nematoda). Hydrobiologia 120, 259-283   DOI
8 Burgess, R. (2001). An improve protocol for separating meiofauna from sediments using colloidal silica sols. Mar. Ecol. Prog. Ser. 214, 161-165   DOI
9 Nelles, L., Fang, B.L., Volckaert, G., Vandenberghe, A., and De Wachter,R. (1984). Nucleotide sequence of a crustacean 18S ribosomal RNA gene and secondary structure of eukaryotic small subunit ribosomal RNAs. Nucleic Acids Res 12, 8749-8768   DOI   ScienceOn
10 Decraemer, W. (1977). Origin and evolution of the Desmoscolecida, an aberrant group of nematodes. J. Zool. Syst. Evol. Res. 15, 232-236   DOI
11 Timm, R.W. (1970). A revision of the nematode order Desmoscolecida Filipjev. (1929). Univ. Cali. Public. Zool. 93, 1-99
12 Filipjev, I.N. (1918). Free-living marine nematodes of the Sevastopol area. Transactions of the Zoological Laboratory and the Sevastopol Biological Station of the Russian Academy of Sciences Series II No. 4 (Issue I and II) (Translated from Russian)
13 Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 17, 540-552   DOI   PUBMED   ScienceOn
14 Andr$\ssy, I. (1976). Evolution as a Basis for the Systematization of Nematodes (London: Pitman Publishing)
15 De Ley, P., and Blaxter, M.L. (2004). A new system for Nematoda: combining morphological characters with molecular trees, and translating clades into ranks and taxa. In Nematology Monographs and Perspectives 2, R. Cook, and D.J. Hunt, eds. (Brill), pp. 633-653
16 Kristensen, R.M. (1989). Marine Tardigrada from the southeastern United States coastal waters I. Paradoxipus orzeliscoides n. gen., n. sp. (Arthrotardigrada: Halechiniscidae). Trans. Am. Micros. Soc. 108, 262-282   DOI   ScienceOn
17 Cook, A.A., Bhadury, P., Debenham, N.J., Meldal, B.H.M., Blaxter, M.L., Smerdon, G., Austen, M.C., Lambshead, P.J.D., and Rogers, A.D. (2005). Denaturing gradient gel electrophoresis (DGGE) as a tool for the identification of marine nematodes. Mar. Ecol. Prog. Ser. 291, 103-113   DOI
18 De Ley, P., De Ley, I.T., Morris, K., Abebe, E., Mundo-Ocampo, M., Yoder, M., Heras, J., Waumann, D., Rocha-Olivares, A., Jay Burr, A.H., et al. (2005). An integrated approach to fast and informative morphological vouchering of nematodes for appli-cations in molecular barcoding. Philos. Trans. R. Soc. Lond., B Biol. Sci 360, 1945-1958   DOI   ScienceOn
19 Lorenzen, S. (1994). The phylogenetic systematics of freeliving nematodes (London: The Ray Society)
20 Posada, D., and Crandall, K.A. (1998). Modeltest: testing the model of DNA substitution. Bioinform. Appl. Notes 14, 817-818   DOI   ScienceOn
21 Litvaitis, M.K., Bates, J.W., Hope, W.D., and Moens, T. (2000). Inferring a classification of the Adenophorea (Nematoda) from nucleotide sequences of the D3 expansion segment (26/28S rDNA). Can. J. Zool. 78, 911-922   DOI   ScienceOn
22 Meldal, B.H.M., Debenham, N.J., De Ley, P., De Ley, I.T., Vanfleteren, J.R., Vierstraete, A.R., Bert, W., Borgonie, G., Moens, T., Tyler, P.A., et al. (2007). An improved molecular phylogeny of the Nematoda with special emphasis on marine taxa. Mol. Phylogenet. Evol 42, 622-636   DOI   ScienceOn
23 Ronquist, F., and Huelsenbeck, J.P. (2003). MrBayes 2: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574   DOI   ScienceOn
24 Cobb, N.A. (1920). One hundred new nemas. Contrib. Sci. Nematol., IX. (Baltimore, USA), pp. 217-343
25 Decraemer, W. (1996). New information on the ultrastructure of the lip region in the genus Desmoscolex and description of Desmoscolex (Desmoscolex) parvospiculatus sp. n. (Nemata: Desmoscolecida) from Papua New Guinea. Nematologica 42, 9-23   DOI
26 Tchesunov, A.V. (1994). On the morphology and systematic position of the family Meyliidae (Nematoda: Chromadoria). Nematologica 40, 369-378   DOI
27 Freudenhammer, I. (1975). Desmoscolecida aus der Iberichen Tiefsee, zugleich eine Revision deiser Nematoden-ordnung. 'Meteor' Forschungsschiffe-Ergebnisse 20, 1-65
28 De Coninck, L.A.P. (1965). Syst$\matique des N$\matodes. Grasse, P.-P. Ed., Traite de Zoologie 4, 1-731
29 Gerlach, S.A., and Riemann, F. (1973; 1974). The Bremerhaven checklist of aquatic nematodes. A catalogue of Nematoda - Adenophorea excluding the Dorylaimida. Veroffentlichungen des Institutes fur Meeresforschung in Bremerhaven, Son-derdruck Q, 1-404 (Part I., 1973), 405-734 (Part II, 1974)
30 Lorenzen, S. (1969). Desmoscoleciden (eine Gruppe freilebender Meeresnematoden) aus Kustensalzwiesen. Veroff. Inst. Meeresforsch. Bremerh. 12, 231-265
31 Kampfer, S., Sturmbauer, C., and Ott, J. (1998). Phylogenetic analysis of rDNA sequences from adenophorean nematodes and implications for the Adenophorea-Secernentea controversy. Invertebr. Biol. 117, 29-36   DOI   ScienceOn
32 Chitwood, B.G. (1933). A revised classification of the Nematoda. J. Parasitol. 20, 131
33 Decraemer, W., and Smol, N. (2006). Orders Chromadorida, Desmodorida and Desmoscolecida. In Freshwater Nematodes, E. Abebe, I. Andrassy, and W. Traunspurger, eds. (CABI Publishing), pp. 555-573
34 De Ley, P., and Blaxter, M.L. (2002). Systematic position and phylogeny. In The Biology of Nematodes, D. Lee, ed. (Harwood Academic Publishers), pp. 1-30
35 Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792-1797   DOI   PUBMED   ScienceOn
36 Filipjev, I.N. (1921). Free-living marine nematodes of the Sevastopolarea. Trudy Osob. Zool. Lab. Sebastop. Biol. Sta. Series II 4, 351-614
37 Lorenzen, S. (1981). Entwurf eines phylogenetischen systems der freilebenden nematoden. Veroffentlichungen des Institut fur Meeresforschungen Bremerhaven, Sonderdruck 7, 1-472
38 Platt, H.M., and Warwick, R.M. (1983). Freeliving marine ne-matodes: Part I British enoplids. (Cambridge University Press)
39 Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., and Helder, J. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Mol. Biol. Evol. 23, 1792-1800   DOI   ScienceOn
40 Aleshin, V.V., Kedrova, O.S., Milyutina, I.A., Vladychenskaya, N.S., and Petrov, N.B. (1998). Relationships among nematodes based on the analysis of 18S rRNA gene sequences: molecular evidence for monophyly of chromadorian and secernentian nematodes. Russ. J. Nematol 6, 175-184
41 Holterman, M., Holovachov, O., van den Elsen, S., van Megen, H., Bongers, T., Bakker, J., and Helder, J. (2008). Small subunit ribosomal DNA-based phylogeny of basal Chromadoria (Nematoda) suggests that transitions from marine to terrestrial habitats (and vice verse) require relatively simple adaptations. Mol. Phylogenet. Evol. 48, 758-763   DOI   ScienceOn
42 Filipjev, I.N. (1929). Les nematodes libres de la baie de la neva et de l'extremete orientale de Golfe de Finlande. Premiere partie. Arch. Hydrobiol. 20, 637-699
43 Swofford, D.L. (2002). PAUP*: Phylogenetic Analysis Using Parsimony (* and other methods). Version 4, Sinauer Associates, Sunderland, MA, USA (software program)