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http://dx.doi.org/10.1007/s10059-009-0057-4

Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L.  

Ondrej, Vladan (Department of Botany, Faculty of Science, Palacky University)
Kitner, Miloslav (Department of Botany, Faculty of Science, Palacky University)
Dolezalova, Ivana (Department of Botany, Faculty of Science, Palacky University)
Nadvornik, Petr (Department of Cell Biology and Genetics, Faculty of Science, Palacky University)
Navratilova, Bozena (Department of Botany, Faculty of Science, Palacky University)
Lebeda, Ales (Department of Botany, Faculty of Science, Palacky University)
Publication Information
Molecules and Cells / v.27, no.4, 2009 , pp. 443-447 More about this Journal
Abstract
This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a higher variability in the expression profile than did leaves' mesophyll cells and protoplasts.
Keywords
cell cycle; chromatin; plant protoplasts; subtelomeric repeats;
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1 Bartova, E., Krejci, J., Harnicarova, A., Galiova, G., and Kozubek, S. (2008). Histone modifications and nuclear architecture: A review. J. Histochem. Cytochem. 56, 711-721   DOI   ScienceOn
2 Koo, D.H., Hur, Y., Jin, D.C., and Bang, J.W. (2002). Karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) using C-banding and bicolor fluorescence in sktu hybridization. Mol. Cells 13, 413-418
3 Kozubek, S., Lukasova E., Jirsova, P., Koutna, I., Kozubek, M., Ganova A., Bartova, E., Falk, M., and Pasekova, R. (2002). 3D structure of the human genome: order in randomness. Chromosoma 111, 321-331   DOI   ScienceOn
4 Papadakis, A.K., and Roubelakis-Angelakis, K.A. (2002). Oxidative stress could be responsible for the recalcitrance of plant protoplasts. Plant Physiol. Biochem. 40, 549-559
5 Tessadori, F., van Driel, R., and Fransz, P. (2004). Cytogenetics as a tool to study gene regulation. Trends Plant Sci. 9 147-153   DOI   ScienceOn
6 Tessadori, F., Chupeau, M.C., Chupeau, Y., Knip, M., Germann, S., van Driel, R., Fransz, P., and Gaudin, V. (2007). Large-scale dissociation and sequential reassembly of pericentric heterochromatin in dedifferentiated Arabidopsis cells. J. Cell Sci. 120, 1200-1208   DOI   ScienceOn
7 Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Pelema, P., and Kuiper, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23, 4407-4414   DOI   ScienceOn
8 Debeaujon, I., and Branchard, M. (1992). Induction of somatic embryogenesis and caulogenesis from cotyledon and leaf protoplast-derived colonies of melon (Cucumis melo L.). Plant Cell Rep. 12, 37-40
9 Vera-Estrella, R., Higgins, V.J., and Blumwald, E. (1994). Plant defense response to fungal pathogens. Plant Physiol. 106, 97-102   DOI
10 Exner, V., and Hennig, L. (2008). Chromatin rearrangements in development. Curr. Opin. Plant Biol. 11, 64-69   DOI   ScienceOn
11 Yang, X., Tu, L., Zhu, L., Fu, L., Min, L., and Zhang, X. (2008). Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by suppression subtractive hybridization and macroarray. J. Exp. Bot. 59, 3661-3674   DOI   ScienceOn
12 Mathieu, O., Jasencakova, Z., Vaillant, I., Gendrel, A.V., Colot, V., Schubert, I., and Tourmente, S. (2003). Changes in 5S rDNA chromatin organization and transcription during heterochromatin establishment in Aranidopsis. Plant Cell 15, 2929-2939   DOI   ScienceOn
13 van Driel, R., and Fransz, P. (2004). Nuclear architecture and genome functioning in plants and animals: what can we learn from both? Exp. Cell Res. 296, 86-90   DOI   ScienceOn
14 Ond$\check{r}$ej, V., Luk$\acute{a}$$\check{s}$ov$\acute{a}$, E., Krej$\check{c}$$\acute{i}$, J., Matula, P., and Kozubek, S.(2008). Lamin A/C and polymeric actin in genome organization. Mol. Cells OS, 356-361
15 Milla, M.A.R., Maurer, A., Huete, A.R., and Gustafson, J.P. (2003). Glutathione peroxidise genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signalling pathways. Plant J. 36, 602-615   DOI   ScienceOn
16 Cremer, T., and Cremer, C. (2001). Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292-301   DOI   ScienceOn
17 Zhao, J., Morozova, N., Williams, L., Libs, L., Avivi, Y., and Grafi, G. (2001). Two phases of chromatin decondensation of plant cells. J. Biol. Chem. 276, 22772-22778   DOI   ScienceOn
18 Koo, D.H., Choi, H.W., Cho, J., Hur, Y., and Bang, J.W. (2005). A high-resolution karyotype of cucumber (Cucumis sativus L. Winter Long) revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization. Genome 48, 534-540   DOI   ScienceOn
19 Cocking, E.C. (1960). A method for the isolation of plant protoplasts and vacuoles. Nature 187, 927-929
20 Lebeda, A., Widrlechner, M.P., Staub, J., Ezura, H., Zalapa, J., and Kristkova E. (2007). Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.). In Genetic Resources, Chromosome Engineering and Crop Improvement Series, Vol. 3 - Vegetable Crops, R.J. Singh, ed. (USA: CRC Press), pp. 271-376
21 Verbsky, M.L., and Richards, E.J. (2001). Chromatin remodeling in plants. Curr. Opin. Plant Biol. 4, 494-500   DOI   ScienceOn
22 Kitner, M., Lebeda, A., Dolezalova, I., Maras, M., Kristkova E., Nevo, E., Pavlicek, T., Meglic, V., and Beharav, A. (2008). AFLP analysis of Lactuca saligna germplasm collections from four European and three Middle Eastern countries. Israel J. Plant Sci. (in press)
23 Annis, S.L., and Goodwin, P.H. (1997). Recent advances in the molecular genetics of plant cell wall-degrading enzymes produced by plant pathogenic fungi. Eur. J. Plant. Pathol. 103, 1-14   DOI   ScienceOn
24 Chen, J.Z., and Tian, L. (2007). Roles of dynamic and reversible histone acetylation in plant development and polyploidy. Biochim. Biophys. Acta 1769, 295-307   DOI   ScienceOn
25 Berta, G.L., Fusconi, A., Sampo, S., Lingua, G., Perticone, S., and Repetto, O. (2000). Polyploidy in tomato roots as affected by arbuscular mycorrhizal colonization. Plant Soil 226, 37-44   DOI   ScienceOn
26 Sablowski, R. (2007). Flowering and determinancy in Arabidopsis. J. Exp. Bot. 58, 899-907   DOI   PUBMED   ScienceOn
27 Ganal, M., Riede, I., and Hemleben, V. (1986). Organization and sequence analysis of two related satellite DNAs in cucumber (Cucumis satiuvus L.). J. Mol. Evol. 23, 23-30   DOI
28 Jenuwein, T., and Allis, C.D. (2001). Translating the histone code. Science 293, 1074-1080   DOI   ScienceOn
29 Takebe, I., and Otsuki, Y. (1969). Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc. Natl. Acad. Sci. USA 64, 843-848   DOI   ScienceOn
30 Gajdova, J., Navratilova, B., Smolna, J., and Lebeda, A. (2007). Factor affecting protoplast isolation and cultivation of Cucumis spp. J. Appl. Bot. Food Quality 81, 1-6
31 Goren, A., and Cedar, H. (2003). Replicating by the clock. Nat. Rev. Mol. Cell. Biol. 4, 25-32   DOI   ScienceOn
32 Pecinka, A., Schubert, V., Maister, A., Kreth, G., Klatte, M., Lysak, M.A., Fuchs, J., and Schubert, I. (2004). Chromosome territory arrangement and homologous pairing in nuclei of Arabidopsis thaliana are predominantly random excerpt for NOR-bearing chromosomes. Chromosoma 113, 258-269   DOI   ScienceOn