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http://dx.doi.org/10.1007/s10059-009-0053-8

Leukotriene B4 Regulates Proliferation and Differentiation of Cultured Rat Myoblasts via the BLT1 Pathway  

Sun, Ru (Institute of Genetics and Cytology, Northeast Normal University)
Ba, Xueqing (Institute of Genetics and Cytology, Northeast Normal University)
Cui, Lingling (Institute of Genetics and Cytology, Northeast Normal University)
Xue, Yan (Institute of Genetics and Cytology, Northeast Normal University)
Zeng, Xianlu (Institute of Genetics and Cytology, Northeast Normal University)
Publication Information
Molecules and Cells / v.27, no.4, 2009 , pp. 403-408 More about this Journal
Abstract
Skeletal muscle regeneration is a highly orchestrated process initiated by activation of adult muscle satellite cells. Upon muscle injury, the inflammatory process is always accompanied by muscle regeneration. Leukotriene $B_4$ is one of the essential inflammatory mediators. We isolated and cultured primary satellite cells. RT-PCR showed that myoblasts expressed mRNA for $LTB_4$ receptors BLT1 and BLT2, and $LTB_4$ promoted myoblast proliferation and fusion. Quantitative real-time PCR and immunoblotting showed that $LTB_4$ treatment expedited the expression process of differentiation markers MyoD and M-cadherin. U-75302, a specific BLT1 inhibitor, but not LY2552833, a specific BLT2 inhibitor, blocked proliferation and differentiation of myoblasts induced by $LTB_4$, which implies the involvement of the BLT1 pathway. Overall, the data suggest that $LTB_4$ contributes to muscle regeneration by accelerating proliferation and differentiation of satellite cells.
Keywords
BLT1; BLT2; $LTB_4$; M-cadherin; MyoD; satellite cells;
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