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http://dx.doi.org/10.1007/s10059-009-0026-y

Inhibition of Homodimerization of Toll-like Receptor 4 by 6-Shogaol  

Ahn, Sang-Il (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University)
Lee, Jun-Kyung (Cell Culture Process Research and Development Center, Celltrion Inc.)
Youn, Hyung-Sun (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University)
Publication Information
Molecules and Cells / v.27, no.2, 2009 , pp. 211-215 More about this Journal
Abstract
Toll-like receptors (TLRs) play a critical role in sensing microbial components and inducing innate immune and inflammatory responses by recognizing invading microbial pathogens. Lipopolysaccharide-induced dimerization of TLR4 is required for the activation of downstream signaling pathways including nuclear factor-kappa B ($NF-{\kappa}B$). Therefore, TLR4 dimerization may be an early regulatory event in activating ligand-induced signaling pathways and induction of subsequent immune responses. Here, we report biochemical evidence that 6-shogaol, the most bioactive component of ginger, inhibits lipopolysaccharide-induced dimerization of TLR4 resulting in the inhibition of $NF-{\kappa}B$ activation and the expression of cyclooxygenase-2. Furthermore, we demonstrate that 6-shogaol can directly inhibit TLR-mediated signaling pathways at the receptor level. These results suggest that 6-shogaol can modulate TLR-mediated inflammatory responses, which may influence the risk of chronic inflammatory diseases.
Keywords
cyclooxygenase-2; dimerization; lipopolysaccharide; $NF-{\kappa}B$; toll like receptors;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 17  (Related Records In Web of Science)
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