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Identification of a Cryptic Type III Polyketide Synthase (1,3,6,8-Tetrahydroxynaphthalene Synthase) from Streptomyces peucetius ATCC 27952  

Ghimire, Gopal Prasad (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
Oh, Tae-Jin (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
Liou, Kwangkyoung (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
Sohng, Jae Kyung (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
Publication Information
Molecules and Cells / v.26, no.4, 2008 , pp. 362-367 More about this Journal
Abstract
We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydroxynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).
Keywords
1,3,6,8-tetrahydroxynaphthalene synthase; chalcone synthase; heterologous expression; Streptomyces peucetius;
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