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Lamin A/C and Polymeric Actin in Genome Organization  

Ondrej, Vladan (Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic)
Lukasova, Emilie (Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic)
Krejci, Jana (Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic)
Matula, Pavel (Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University)
Kozubek, Stanislav (Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic)
Publication Information
Molecules and Cells / v.26, no.4, 2008 , pp. 356-361 More about this Journal
Abstract
In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, and organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation of lamin A/C expression led to disruption of the lamin A/C perinuclear layer and disorganization of lamin-bound emerin complexes at the inner nuclear membrane. The silencing of lamin A/C expression resulted in a decrease in the volume and surface area of chromosome territories, especially in chromosomes with high heterochromatin content. Inhibition of actin polymerization led to relaxation of the structure of chromosome territories, and an increase in the volumes and surface areas of the chromosome territories of human chromosomes 1, 2 and 13. The results show an important role of polymeric actin in the organization of the nuclei and the chromosome territories.
Keywords
chromosome territories; lamin A/C; nuclear organization; polymeric actin;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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1 Guelen, L., Pagie, L., Brasset, E., Meuleman, W., Faza, M.B., Talhout, W., Eussen, B.H., de Klein, A., Wessels, L., de Laat, W., et al. (2008). Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Nature 453, 948-951   DOI   ScienceOn
2 Hofmann, W., Reichart, B., Ewald, A., Müller, E., Schmitt, I., Stauber, R.H., Lottspeich, F., Jockusch, B.M., Scheer, U., Hauber, J., et al. (2001). Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin. J. Cell Biol. 152, 895-910   DOI
3 Holaska, J.M., Wilson, K.L., and Mansharami, M. (2002). The nuclear envelope, lamins and nuclear assembly. Curr. Opin. Cell Biol. 14, 357-364   DOI   ScienceOn
4 Kiseleva, E., Drummond, S.P., Goldberg, M.W., Rutheford, S.A., Allen, T.D., and Wilson, K.L., (2004). Actin- and protein-4.1- containing filaments link nuclear pore complexes to subnuclear organelles in Xenopus oocyte nuclei. J. Cell Sci. 117, 2481-2490   DOI   ScienceOn
5 Libotte, T., Zaim, H., Abraham, S., Padmakumar, V.C., Schneider, M., Lu, W., Munck, M., Hutchinson, Ch., Wehnert, M., Fahrenkrog, B., et al. (2005). Lamin A/C-dependent localization of nesprin-2, a giant scaffolder at the nuclear envelope. Mol. Biol. Cell 16, 3411-3424   DOI   ScienceOn
6 Lin, F., and Worman H.J. (1993). Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C. J. Biol. Chem. 268, 16321-16326
7 Caron, H., van Schaik, B., van der Mee, M., Baas, F., Riggins, G., van Sluis, P., Hermus, M.Ch., van Asperen, R., Boon, K., Voute, P.A., et al. (2001). The human transcriptome map: Clustering of highly expressed genes in chromosomal domains. Science 291, 1289-1292   DOI   ScienceOn
8 Kozubek, S., Lukasova, E., Jirsova, P., Koutna, I., Kozubek, M., Gaňova, A., Bartova, E., Falk, M., and Pasekova, R. (2002). 3D structure of the human genome: order in randomness. Chromosoma 111, 321-331   DOI
9 Vlcek S., and Foisner R. (2007). Lamins and lamin-associated proteins in aging and disease. Curr. Opin. Cell Biol. 19, 298-304   DOI   ScienceOn
10 Bettinger, B.T., Gilbert, D.M., and Amber, D.C. (2004). Actin up in the nucleus. Mol. Cell. Biol. 5, 410-415
11 Segura-Totten, M., and Wilson, K.L. (2004). BAF: roles in chromatin, nuclear structure and retrovirus integration. Trends Cell Biol. 14, 261-266   DOI   ScienceOn
12 Hofmann, W., Stojiljkovic, L., Fuchsova, B., Vargas, G.M., Mavrommatis, E., Philimonenko, V., Kysela, K., Goodrich, J.A., Lessard, J.L., Hope, T.J., et al. (2004). Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat. Cell. Biol. 6, 1094-1101   DOI   ScienceOn
13 Albiez, H., Cremer, M., Tieri, C., Vecchio, L., Schermelleh, L., Dittrich, S., Küpper, K., Joffe, B., Thormeyer, T., von Hase, J., et al. (2006). Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks. Chrom. Res. 14, 707-733   DOI   ScienceOn
14 Croft, J., Bridger, J., Perry, P., Boyle, S., Teague, P., and Bickmore, W. (1999). Differences in the localization and morphology of chromosomes in the human nucleus. J. Cell Biol. 145, 1119-1131   DOI
15 Holaska, J.M., Kowalski, A.K., and Wilson, K.L. (2004). Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane. PLoS Biol 2, e231   DOI   ScienceOn
16 Goetze, S., Mateos-Langerak, J., Gierman, H.J., de Leeuw, W., Giromus, O., Indemans, M.H.G., Koster, J., Ondrej, V., Versteeg, R., and van Driel, R. (2007). The 3D structure of human interphase chromosome is related to the transcriptome map. Mol. Cell. Biol. 27, 4475-4487   DOI   ScienceOn
17 Bolzer, A., Kreth, G., Solovei, I., Koehler, D., Saracoglu, K., Fauth, Ch., Muller, S., Eils, R., Cremer, Ch., Speicher, M.R., et al. (2005). Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes. PLoS Biol. 3, e157   DOI   ScienceOn
18 Cremer, T., and Cremer, C. (2001). Chromosome territories, nuclear architecture and gene regulation in mammalian cells. Nat. Rev. Genet. 2, 292-301   DOI   ScienceOn
19 McDonald, D., Carrero, G., Andrin, Ch., de Vries, G., and Hendzel, M.J., (2006). Nucleoplasmic $\beta$-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations. J. Cell Biol. 172, 541-552   DOI   ScienceOn
20 Le Beyec, J., Xu, R., Lee, S.Y., Nelson, C.M., Ritzki, A., Alcaraz, J., and Bissell, M.J. (2007). Cell shape regulates global histone acetylation in human mammary epithelial cells. Exp. Cell Res. 313, 3066-3075   DOI   ScienceOn
21 Moir, R.D., Spann, T.P., Herrmann, H., and Goldman, R.D. (2000). Disruption of nuclear lamin organization blocks the elongation phase of DNA replication. J. Cell Biol. 149, 1179-1191   DOI
22 Boyle, S., Gilchrist, S., Bridger, J.M., Mahy, N.L., Ellis, J.A., and Bickmore, W.A. (2001). The spatial organization of human chromosomes within the nuclei of normal and emerin-mutant cells. Hum. Mol. Genet. 10, 211-219   DOI   ScienceOn
23 Misteli, T. (2007). Beyond the sequence: cellular organization of genome function. Cell 128, 787-800   DOI   ScienceOn
24 Meaburn, K.J., Misteli, T., and Soutoglou, E. (2007). Spatial genome organization in the formation of chromosomal translocations. Sem. Cancer Biol. 17, 80-90   DOI   ScienceOn
25 Lee, K.K., Haraguchi, T., Lee, R.S., Koujin, T., Hiraoka, Y., and Wilson, K.L. (2001). Distinct functional domains in emerin bind lamin A and DNA-bridging protein BAF. J. Cell Sci. 114, 4567-4573
26 Shumaker, D.K., Kuczmarski, E.R., and Goldman, R.D. (2003). The nucleoskeleton: lamins and actin are major players in essential nuclear functions. Curr. Opin. Cell Biol. 15, 358-366   DOI   ScienceOn
27 Dunham, A., Matthews, L.H., Burton, J., Ashurst, J.L., Howe, K.L., Ashcroft, K.J., Beare, D.M., Burford, D.C., Hunt, S.E., Griffiths- Jones, S., et al. (2004). The DNA sequence and analysis of human chromosome 13. Nature 428, 522-528   DOI   ScienceOn
28 Kozubek, M., Kozubek, S., Lukašova, E., Bartova, E., Skalníkova, M., Matula, P., Matula, P., Jirsova, P., Cafourkova, A., and Koutna, I. (2001). Combined confocal and wide-field highresolution citometry of fluorescence in situ hybridization-stained cells. Cytometry 45, 1-12   DOI   ScienceOn