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Repression of Transcriptional Activity of Estrogen Receptor α by a Cullin3/SPOP Ubiquitin E3 Ligase Complex  

Byun, Boohyeong (Department of Oriental Medicine, Daegu Haany University)
Jung, Yunhwa (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Publication Information
Molecules and Cells / v.25, no.2, 2008 , pp. 289-293 More about this Journal
Abstract
The role of SPOP in the ubiquitination of $ER{\alpha}$ by the Cullin3-based E3 ubiquitin ligase complex was investigated. We showed that the N-terminal region of SPOP containing the MATH domain interacts with the AF-2 domain of $ER{\alpha}$ in cultured human embryonic 293 cells. SPOP was required for coimmunoprecipitation of $ER{\alpha}$ with Cullin3. This is the first report of the essential role of SPOP in $ER{\alpha}$ ubiquitination by the Cullin3-based E3 ubiquitin ligase complex. We also demonstrated repression of the transactivation capability of $ER{\alpha}$ in cultured mammalian cells.
Keywords
Cullin3-based E3 Ubiquitin Ligase Complex; $ER{\alpha}$; SPOP; Ubiquitination;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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