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Low-Dose Radiation Stimulates the Proliferation of Normal Human Lung Fibroblasts Via a Transient Activation of Raf and Akt  

Kim, Cha Soon (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Kim, Jin Kyoung (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Nam, Seon Young (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Yang, Kwang Hee (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Jeong, Meeseon (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Kim, Hee Sun (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Kim, Chong Soon (Korea Institute of Radiological and Medical Sciences)
Jin, Young-Woo (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd.)
Kim, Joon (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Molecules and Cells / v.24, no.3, 2007 , pp. 424-430 More about this Journal
Abstract
The biological effects of low-dose radiation have been investigated and debated for more than a century, but its cellular effects and regulatory mechanisms remain poorly understood. This study shows the human cellular responses to low-dose radiation in CCD-18 Lu cells, which are derived from normal human lung fibroblasts. We examined a colony-forming assay for cell survival by ionizing radiation. Live cell counting and cell cycle analysis were measured for cell proliferation and cell cycle progression following low-dose irradiation. We examined Raf and Akt phosphorylation to determine the proliferation mechanism resulting from low-dose radiation. We also observed that p53 and p21 were related to cell cycle response. We found that 0.05 Gy of ionizing radiation enhanced cell proliferation and did not change the progression of the cell cycle. In addition, 0.05 Gy of ionizing radiation transiently activated Raf and Akt, but did not change phospho-p53, p53 and p21 in CCD-18 Lu cells. However, 2 Gy of ionizing radiation induced cell cycle arrest, phosphorylation of p53, and expression of p53 and p21. The phosphorylation of Raf and Akt proteins induced by 0.05 Gy of ionizing radiation was abolished by pre-treatment with an EGFR inhibitor, AG1478, or a PI3k inhibitor, LY294002. Cell proliferation stimulated by 0.05 Gy of ionizing radiation was blocked by the suppression of Raf and Akt phosphorylation with these inhibitors. These results suggest that 0.05 Gy of ionizing radiation stimulates cell proliferation through the transient activation of Raf and Akt in CCD-18 Lu cells.
Keywords
Akt; Cell Proliferation; Low-Dose Radiation; Normal Human Diploid Cells; Raf;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 12  (Related Records In Web of Science)
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