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Identification of a Novel Rb-regulated Gene Associated with the Cell Cycle  

Sung, Young Hoon (Department of Biochemistry and Yonsei Laboratory Animal Research Center, Yonsei University)
Kim, Hye Jin (Department of Biochemistry and Yonsei Laboratory Animal Research Center, Yonsei University)
Lee, Han-Woong (Department of Biochemistry and Yonsei Laboratory Animal Research Center, Yonsei University)
Publication Information
Molecules and Cells / v.24, no.3, 2007 , pp. 409-415 More about this Journal
Abstract
The retinoblastoma (Rb) gene is one of the most important genes in cell cycle regulation and tumorigenesis. Homozygosity for a germ-line Rb mutation results in embryonic lethality and evokes developmental defects associated with inappropriate S-phase entry and high levels of apoptosis. Although Rb has been extensively studied, more target genes need to be identified and characterized to unravel the precise mechanism of Rb function. In order to identify Rb-regulated genes, we analyzed the gene expression profile of Rb-deficient mouse embryo fibroblasts (MEFs), and identified an unknown gene, RbEST47, that is transcriptionally upregulated in Rb-deficient MEFs. This gene is conserved from fruitfly to human. It is expressed in brain, lung, kidney, and testis, and is located on mouse chromosome 2. This region is syntenic to human chromosome 9q34.3, which frequently exhibits loss of heterozygosity in neoplastic diseases. RbEST47 was considerably down-regulated in immortalized cells, and showed cell cycle-dependent expression, suggesting important roles in S and/or G2.
Keywords
Cancer; Cell Cycle; E2F; Immortalization; Microarray; Rb;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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