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Association of BAF53 with Mitotic Chromosomes  

Lee, Kiwon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Shim, Jae Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Kang, Mi Jin (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Kim, Ji Hye (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Ahn, Jong-Seong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Yoo, Soon Ji (Department of Biology, Kyunghee University)
Kim Kwon, Yunhee (Department of Biology, Kyunghee University)
Kwon, Hyockman (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Publication Information
Molecules and Cells / v.24, no.2, 2007 , pp. 288-293 More about this Journal
Abstract
The conversion of mitotic chromosome into interphase chromatin consists of at least two separate processes, the decondensation of the mitotic chromosome and the formation of the higher-order structure of interphase chromatin. Previously, we showed that depletion of BAF53 led to the expansion of chromosome territories and decompaction of the chromatin, suggesting that BAF53 plays an essential role in the formation of higher-order chromatin structure. We report here that BAF53 is associated with mitotic chromosomes during mitosis. Immunostaining with two different anti-BAF53 antibodies gave strong signals around the DNA of mitotic preparations of NIH3T3 cells and mouse embryo fibroblasts (MEFs). The immunofluorescent signals were located on the surface of mitotic chromosomes prepared by metaphase spread. BAF53 was also found in the mitotic chromosome fraction of sucrose gradients. Association of BAF53 with mitotic chromosomes would allow its rapid activation on the chromatin upon exit from mitosis.
Keywords
Actin-related Protein; BAF53; Decondensation; Higher-Order Chromatin Structure; Mitosis; Mitotic Chromosome;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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